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The map label for this gene is yogA [H]

Identifier: 84622982

GI number: 84622982

Start: 1447141

End: 1447854

Strand: Direct

Name: yogA [H]

Synonym: XOO_1325

Alternate gene names: 84622982

Gene position: 1447141-1447854 (Clockwise)

Preceding gene: 84622980

Following gene: 84622983

Centisome position: 29.29

GC content: 64.15

Gene sequence:

>714_bases
TTGGTCATTTGCCATCCCGCCGCGATCGCATCGCTACAGTCGGTTGCATTGCCCGATCCCGGGCAACCTGCGCGCGGTCA
GATCCGTGTGCGTCTGCGTGCCAGCTCGCTCAATTTTCATGATCTGGGCGTTGTGCTCGGGAAGATGTCGACCGCAGACG
GGCGTATTCCGATGTCAGATGGTGCCGGCGTCGTGGAAGCGGTAGGGCCGGAGGTCGAGGAGTTCGCAGTGGGCGATCAT
GTCGTTTCCACCTTCTTTGCGCAGTGGCTGGATGGTGAGCCGTTGCATGCTGGTTTCGCCACCACCCCAGGTGACGGCGT
CGACGGCTACGCGCGCACTGCGGTCGTTGCCGATGCGCAGGCATCCAGCCACGCACCCCGCGGTTATAGCCACGCCGAGG
CCGCGACGTTGACCACCGCCGGAGTTACTGCGTGGCGCGCGCTGGTGGTCAATGGCAGACGCAAGGCCGGCGACAGCGTG
CTGGTGCTGGGTAGCGGTGGCGTGCCGATCTTTGGGCTGCAATTCGCCAACGCGATGGGCGCACGTGTGATCGCCACTTC
ATCATCCGACGAGAAGTTGAAAAAGGTGCGCGCGCTCGGAGCCGAGCACCTCCTCAATTATCGGCAACATCCCGAATGTC
GGCGCAGGTGCTGGACATCACCGAGGGGCGCGGCGTTGACCATGTGGTCGAGGTCGGCGGATCGGGCACGCTGA

Upstream 100 bases:

>100_bases
AAACATCACCAGTGGGCAAGCTAGCTCGACTGCATGCGCGTCATGGCGCGCGTGATGTTCCGTGCGTTGAACCACGCTCA
GGGGTGCCCCATGCAAGCCA

Downstream 100 bases:

>100_bases
CGCAATCAATTCGTGCGGCGCGCGTGGGAGGACATATCGCCTTGATCGGCGTGTTGACCGGTGGGGCTGGAGAGGTGCCG
ACCGCCGACATGATGGGGCG

Product: hypothetical protein

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MVICHPAAIASLQSVALPDPGQPARGQIRVRLRASSLNFHDLGVVLGKMSTADGRIPMSDGAGVVEAVGPEVEEFAVGDH
VVSTFFAQWLDGEPLHAGFATTPGDGVDGYARTAVVADAQASSHAPRGYSHAEAATLTTAGVTAWRALVVNGRRKAGDSV
LVLGSGGVPIFGLQFANAMGARVIATSSSDEKLKKVRALGAEHLLNYRQHPECRRRCWTSPRGAALTMWSRSADRAR

Sequences:

>Translated_237_residues
MVICHPAAIASLQSVALPDPGQPARGQIRVRLRASSLNFHDLGVVLGKMSTADGRIPMSDGAGVVEAVGPEVEEFAVGDH
VVSTFFAQWLDGEPLHAGFATTPGDGVDGYARTAVVADAQASSHAPRGYSHAEAATLTTAGVTAWRALVVNGRRKAGDSV
LVLGSGGVPIFGLQFANAMGARVIATSSSDEKLKKVRALGAEHLLNYRQHPECRRRCWTSPRGAALTMWSRSADRAR
>Mature_237_residues
MVICHPAAIASLQSVALPDPGQPARGQIRVRLRASSLNFHDLGVVLGKMSTADGRIPMSDGAGVVEAVGPEVEEFAVGDH
VVSTFFAQWLDGEPLHAGFATTPGDGVDGYARTAVVADAQASSHAPRGYSHAEAATLTTAGVTAWRALVVNGRRKAGDSV
LVLGSGGVPIFGLQFANAMGARVIATSSSDEKLKKVRALGAEHLLNYRQHPECRRRCWTSPRGAALTMWSRSADRAR

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239676, Length=154, Percent_Identity=34.4155844155844, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI194239674, Length=154, Percent_Identity=34.4155844155844, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI13236495, Length=154, Percent_Identity=34.4155844155844, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323729, Length=208, Percent_Identity=28.8461538461538, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=216, Percent_Identity=25.462962962963, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323961, Length=215, Percent_Identity=24.6511627906977, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 24934; Mature: 24934

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVICHPAAIASLQSVALPDPGQPARGQIRVRLRASSLNFHDLGVVLGKMSTADGRIPMSD
CEEECHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
GAGVVEAVGPEVEEFAVGDHVVSTFFAQWLDGEPLHAGFATTPGDGVDGYARTAVVADAQ
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEEEECCC
ASSHAPRGYSHAEAATLTTAGVTAWRALVVNGRRKAGDSVLVLGSGGVPIFGLQFANAMG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEHHHHHCCC
ARVIATSSSDEKLKKVRALGAEHLLNYRQHPECRRRCWTSPRGAALTMWSRSADRAR
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MVICHPAAIASLQSVALPDPGQPARGQIRVRLRASSLNFHDLGVVLGKMSTADGRIPMSD
CEEECHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
GAGVVEAVGPEVEEFAVGDHVVSTFFAQWLDGEPLHAGFATTPGDGVDGYARTAVVADAQ
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEEEECCC
ASSHAPRGYSHAEAATLTTAGVTAWRALVVNGRRKAGDSVLVLGSGGVPIFGLQFANAMG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEHHHHHCCC
ARVIATSSSDEKLKKVRALGAEHLLNYRQHPECRRRCWTSPRGAALTMWSRSADRAR
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]