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The map label for this gene is btuB [H]

Identifier: 84622892

GI number: 84622892

Start: 1344982

End: 1346859

Strand: Direct

Name: btuB [H]

Synonym: XOO_1235

Alternate gene names: 84622892

Gene position: 1344982-1346859 (Clockwise)

Preceding gene: 84622891

Following gene: 84622894

Centisome position: 27.23

GC content: 65.97

Gene sequence:

>1878_bases
ATGTCCCTTTCTTCCGTTTTGCCGCGTCGTGCCGTGCTCGCCGTGGGGGTGTCGCTGTGCGTGACCAATCTGGCCCAGGC
CGAGGCCGCCACCGATCTCGATCAGATCGTGGTCACCGCCTCGCGCACCGCGCAGACCCAGGACCAGACGCTGGCGCCGG
TCACGGTCATCGATCGCGCGCAGATCGAGCGTCGCCAGGTCAATTCGCTACAGGATCTGTTGCGTGGCGAGGCGGGCGTG
TCGCTGGCCAACAACGGTGGACCCGGCAAGGCGACCTCGCTGTTCCTGCGCGGCACCGAAGCCGACCAGGTGGTGGTGCT
GATCGATGGCGTGCGCATCGGTTCGGCCACTGCCGGCGGCGCGGCGCTGCAGGATCTGCCGATCGAACAGATCGAACGGA
TCGAAATCGTGCGCGGCCCGTTTTCCAGCCTGTACGGCTCCGAAGCGCTGGGCGGGGTGATCCAGATCTTTACCCGGCGC
CCGCAAGGCAGCTTCGTGCCGACCTTGAGCGTGGCGGCTGGCAGCGACAACGCGCGCCGCTACGGCGCGGGCGTGGCCGG
GCGCAGCGCAGGCGATCTGAGCGAATCAGGCGGTTGGTATTCAGTGAATGCCGTGCACGACGAAACCGACGGCATCAACG
CGTATCTGGATACCAGCTCCAGCGCCTACGACCCGGACCGCGATGGCTACCGCAACGATTCGTCGAGCGCGCAGGGCGGC
TGGCGTTTCAATCGCCAGTGGGACGCCGATGTGCATGCGCTGCGCGCACAGAGCCGCAACGAATACGATGGCTCGGCATT
CGGCGGCAATCTGAGCAAGGGCGTGCAGCAGGCCATCGGTGGCCGCCTGCGTTACGCGCCGAGCGATGCGCTCAAACTCA
CCGCCAGCGTCGGCAGCAGCGCCGATCTGAGCGATGCGTATTATGAAGGTGCCTACCTGTCGACCTACGACACCCGCCGC
AAGCAGGGCGCGTTGCAGGCCGATCTCGATACCGGCCCCGGCCTGCTCACGGTCGGCTTCGATTGGCAGCGCGACGCCAT
CGCCAGCAGCGACACCTACGATGCGAACAGCCGTATCGATCGCGCCGCATTTGCGCAGTGGCAGCAGACGCTCGGCAGCC
AATCGCTGCAGGCCAGCCTGCGCCGCAACGACAACAGTCAGTTCGGCGGCAAGACCACCGGCAGCCTGCTGTGGGGTTGG
GATTTCGCCGAGCATCTGCGCGTGACTGCCAGTTACGGCACTGCGTTCAAGGCGCCTACCTTCAACGAGCTGTATTACCC
CGATTACGGCAACCCGCTGCTGGGGCCGGAAACTTCCAGGAGCGCCGAGCTTGGCTTGCGCGGTCGCTATGACTGGGGCA
CCTGGACGCTCAGCGCGTTCCAGACCCGTATCGACGACCTGATTGCCTACGACGCGTCGTTGGTGGATGCGAGCCACCCG
TTCGGGCAGCCGAACAATATCGATCGCGCGCGTATCCGCGGCGTGGAAGCCGGCCATGACACCGCACTGGCCGGCTGGAC
CCTGCGCACCGCCGTGACCTGGCTTGCGCCGCAGGCCGATGGCGACGTCAATCACGGCAACTGGTTGCCGCGGCGTGCGC
GCCAAAGCGGGCGTATCGATGCCGACCGCAGCATTGGTGCATTCGGTGTCGGCGCAAGCCTGTTCGGATCGGGCGCGCGT
TACGACGATCTGGCCAATACCCAACGCCTGGCCGGCGAAGGCGTGCTGGATCTGCGCGCGAGCTATGCAGTGAACGCGGA
CTGGAAGGTGCAGCTCACGGCCAACAATGTGTTCGACCGTCAGTACGAAACGGCACGCTGGTACGCGCAGCCGGGCCGCA
ATTACCTGCTGACCTTGCGCTACGAGCCAGCGCAATAA

Upstream 100 bases:

>100_bases
CCTGTGCATCTGGTGCTCCCGCACCGGACGCCTGGCCCGTTGCTGCCTGCCGACCCCATTGCCGCCGCGCGGGTAGGCGC
GGTTTCAGGAGCAACTGCCG

Downstream 100 bases:

>100_bases
GCGCAGTTCACAAACCGATTGCGCAGCCGCCCGACGGGCGCGGCCGGTGCTCGGAGGCGTTATGTACTACGCGACCACGG
CGGCTCCTGAGCGCTGCCCG

Product: outer membrane receptor for transport of vitamin B

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSLSSVLPRRAVLAVGVSLCVTNLAQAEAATDLDQIVVTASRTAQTQDQTLAPVTVIDRAQIERRQVNSLQDLLRGEAGV
SLANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGGAALQDLPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRR
PQGSFVPTLSVAAGSDNARRYGAGVAGRSAGDLSESGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSSSAQGG
WRFNRQWDADVHALRAQSRNEYDGSAFGGNLSKGVQQAIGGRLRYAPSDALKLTASVGSSADLSDAYYEGAYLSTYDTRR
KQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDANSRIDRAAFAQWQQTLGSQSLQASLRRNDNSQFGGKTTGSLLWGW
DFAEHLRVTASYGTAFKAPTFNELYYPDYGNPLLGPETSRSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDASHP
FGQPNNIDRARIRGVEAGHDTALAGWTLRTAVTWLAPQADGDVNHGNWLPRRARQSGRIDADRSIGAFGVGASLFGSGAR
YDDLANTQRLAGEGVLDLRASYAVNADWKVQLTANNVFDRQYETARWYAQPGRNYLLTLRYEPAQ

Sequences:

>Translated_625_residues
MSLSSVLPRRAVLAVGVSLCVTNLAQAEAATDLDQIVVTASRTAQTQDQTLAPVTVIDRAQIERRQVNSLQDLLRGEAGV
SLANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGGAALQDLPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRR
PQGSFVPTLSVAAGSDNARRYGAGVAGRSAGDLSESGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSSSAQGG
WRFNRQWDADVHALRAQSRNEYDGSAFGGNLSKGVQQAIGGRLRYAPSDALKLTASVGSSADLSDAYYEGAYLSTYDTRR
KQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDANSRIDRAAFAQWQQTLGSQSLQASLRRNDNSQFGGKTTGSLLWGW
DFAEHLRVTASYGTAFKAPTFNELYYPDYGNPLLGPETSRSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDASHP
FGQPNNIDRARIRGVEAGHDTALAGWTLRTAVTWLAPQADGDVNHGNWLPRRARQSGRIDADRSIGAFGVGASLFGSGAR
YDDLANTQRLAGEGVLDLRASYAVNADWKVQLTANNVFDRQYETARWYAQPGRNYLLTLRYEPAQ
>Mature_624_residues
SLSSVLPRRAVLAVGVSLCVTNLAQAEAATDLDQIVVTASRTAQTQDQTLAPVTVIDRAQIERRQVNSLQDLLRGEAGVS
LANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGGAALQDLPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRRP
QGSFVPTLSVAAGSDNARRYGAGVAGRSAGDLSESGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSSSAQGGW
RFNRQWDADVHALRAQSRNEYDGSAFGGNLSKGVQQAIGGRLRYAPSDALKLTASVGSSADLSDAYYEGAYLSTYDTRRK
QGALQADLDTGPGLLTVGFDWQRDAIASSDTYDANSRIDRAAFAQWQQTLGSQSLQASLRRNDNSQFGGKTTGSLLWGWD
FAEHLRVTASYGTAFKAPTFNELYYPDYGNPLLGPETSRSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDASHPF
GQPNNIDRARIRGVEAGHDTALAGWTLRTAVTWLAPQADGDVNHGNWLPRRARQSGRIDADRSIGAFGVGASLFGSGARY
DDLANTQRLAGEGVLDLRASYAVNADWKVQLTANNVFDRQYETARWYAQPGRNYLLTLRYEPAQ

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=638, Percent_Identity=31.974921630094, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1788478, Length=672, Percent_Identity=25.297619047619, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786798, Length=172, Percent_Identity=31.9767441860465, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67430; Mature: 67298

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSSVLPRRAVLAVGVSLCVTNLAQAEAATDLDQIVVTASRTAQTQDQTLAPVTVIDRA
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCEEEHHHH
QIERRQVNSLQDLLRGEAGVSLANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGG
HHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEEECCCCCEEEEEEECEEECCCCCCC
AALQDLPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRRPQGSFVPTLSVAAGSDNARR
CHHHCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHH
YGAGVAGRSAGDLSESGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSSSAQGG
HCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
WRFNRQWDADVHALRAQSRNEYDGSAFGGNLSKGVQQAIGGRLRYAPSDALKLTASVGSS
EEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCHHEEEEECCCCC
ADLSDAYYEGAYLSTYDTRRKQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDANSRID
CCCCHHHHCCCEEECCHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHH
RAAFAQWQQTLGSQSLQASLRRNDNSQFGGKTTGSLLWGWDFAEHLRVTASYGTAFKAPT
HHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHEEEEECCCCEECCCC
FNELYYPDYGNPLLGPETSRSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDASHP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCHHHCCCCCC
FGQPNNIDRARIRGVEAGHDTALAGWTLRTAVTWLAPQADGDVNHGNWLPRRARQSGRID
CCCCCCCCHHHHCCCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCC
ADRSIGAFGVGASLFGSGARYDDLANTQRLAGEGVLDLRASYAVNADWKVQLTANNVFDR
CCCCCCCEECCHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEEECCCCCC
QYETARWYAQPGRNYLLTLRYEPAQ
CHHHHHHEECCCCCEEEEEEECCCC
>Mature Secondary Structure 
SLSSVLPRRAVLAVGVSLCVTNLAQAEAATDLDQIVVTASRTAQTQDQTLAPVTVIDRA
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCEEEHHHH
QIERRQVNSLQDLLRGEAGVSLANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGG
HHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEEECCCCCEEEEEEECEEECCCCCCC
AALQDLPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRRPQGSFVPTLSVAAGSDNARR
CHHHCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHH
YGAGVAGRSAGDLSESGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSSSAQGG
HCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
WRFNRQWDADVHALRAQSRNEYDGSAFGGNLSKGVQQAIGGRLRYAPSDALKLTASVGSS
EEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCHHEEEEECCCCC
ADLSDAYYEGAYLSTYDTRRKQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDANSRID
CCCCHHHHCCCEEECCHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHH
RAAFAQWQQTLGSQSLQASLRRNDNSQFGGKTTGSLLWGWDFAEHLRVTASYGTAFKAPT
HHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHEEEEECCCCEECCCC
FNELYYPDYGNPLLGPETSRSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDASHP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCHHHCCCCCC
FGQPNNIDRARIRGVEAGHDTALAGWTLRTAVTWLAPQADGDVNHGNWLPRRARQSGRID
CCCCCCCCHHHHCCCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCC
ADRSIGAFGVGASLFGSGARYDDLANTQRLAGEGVLDLRASYAVNADWKVQLTANNVFDR
CCCCCCCEECCHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEEECCCCCC
QYETARWYAQPGRNYLLTLRYEPAQ
CHHHHHHEECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA