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The map label for this gene is mphP [H]

Identifier: 84622001

GI number: 84622001

Start: 373979

End: 374701

Strand: Direct

Name: mphP [H]

Synonym: XOO_0344

Alternate gene names: 84622001

Gene position: 373979-374701 (Clockwise)

Preceding gene: 84621996

Following gene: 84622003

Centisome position: 7.57

GC content: 66.8

Gene sequence:

>723_bases
ATGATTGCGCCCACCGTGGCCCACTGCCAGTTCGTCCGTGACGACGGGCAGCCGCTGGATTTCCAGCCCGGCCAGTTCAT
CCAGATCCATTTCGACTACGCCGATGGCACGCCGACCAAGCGCAGCTATTCGCTGGCGACCATCCACGACCACGCGCTGG
GACCGGGCGAGGCGGTGGAGATTGCGGTCAGTTTCGTGCCCGGCGGCTCGGCCACGGCCTTGTTCGAAGGTCTGGAGATC
GGCGACCAGTTGCAGGCAAGCGGGCCCTACGGGCGCTTCTGCCTGCAGCCGGGCGACCACAACCAGCGCTATGTGCTGAT
CGCTACCGGCACCGGCGTGACGCCGTACCGCTCGATGCTGCCGCTGCTGGCCGAGGCGATCGCTACCCGCGGTGTGCAGG
TGGTGTTGCTGCAGGGCGCGCGCAACCCGACCGAGCTGCTGTACGGCGACGATTTCCGCGCATTTGCCGATGCGCATCCG
CAGTTCCGCTACGTGCCGTGCTTCTCGCGCGACGTGCCCGAGCAGCCGCACGCCGACGTGCGGCACGGCTACGTGCAGCA
GCACCTGGCCGAGTTCGTGCCCGATGCTGCGCACGATATCGCCTACCTGTGCGGCAATCCGGACATGGTGGACATCTGCC
TGGAAGCGTTGAAAGCAGCCGGGCTGCCAAGTGCGCAGATCCGTCGCGAGAAGTACGTCAGCTCCACGCCGCCCAAGGTC
TGA

Upstream 100 bases:

>100_bases
GCCCGTTATTATAGAAGCCCGTTGGGGTTTGCCCCGATCACAGACTGTCCCAAATACGTTCGTGCCCGTTCAATTCCCCC
TCAAGCTTGTCGACCGGCGC

Downstream 100 bases:

>100_bases
AGGCGGTGGCCTGCAGGCGGCGCGTGCAAGCCGCCTGTGCCCGCAGTGCGGTGGCCTGCAGCGCGGTGAGGAGTTGCTGC
GGTACTAGGCCGCCGCGTTC

Product: phenol hydroxylase

Products: NA

Alternate protein names: Phenol 2-monooxygenase P5 component [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MIAPTVAHCQFVRDDGQPLDFQPGQFIQIHFDYADGTPTKRSYSLATIHDHALGPGEAVEIAVSFVPGGSATALFEGLEI
GDQLQASGPYGRFCLQPGDHNQRYVLIATGTGVTPYRSMLPLLAEAIATRGVQVVLLQGARNPTELLYGDDFRAFADAHP
QFRYVPCFSRDVPEQPHADVRHGYVQQHLAEFVPDAAHDIAYLCGNPDMVDICLEALKAAGLPSAQIRREKYVSSTPPKV

Sequences:

>Translated_240_residues
MIAPTVAHCQFVRDDGQPLDFQPGQFIQIHFDYADGTPTKRSYSLATIHDHALGPGEAVEIAVSFVPGGSATALFEGLEI
GDQLQASGPYGRFCLQPGDHNQRYVLIATGTGVTPYRSMLPLLAEAIATRGVQVVLLQGARNPTELLYGDDFRAFADAHP
QFRYVPCFSRDVPEQPHADVRHGYVQQHLAEFVPDAAHDIAYLCGNPDMVDICLEALKAAGLPSAQIRREKYVSSTPPKV
>Mature_240_residues
MIAPTVAHCQFVRDDGQPLDFQPGQFIQIHFDYADGTPTKRSYSLATIHDHALGPGEAVEIAVSFVPGGSATALFEGLEI
GDQLQASGPYGRFCLQPGDHNQRYVLIATGTGVTPYRSMLPLLAEAIATRGVQVVLLQGARNPTELLYGDDFRAFADAHP
QFRYVPCFSRDVPEQPHADVRHGYVQQHLAEFVPDAAHDIAYLCGNPDMVDICLEALKAAGLPSAQIRREKYVSSTPPKV

Specific function: Probable electron transfer from NADPH, via FAD and the 2Fe-2S center, to the oxygenase activity site of the enzyme [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=229, Percent_Identity=30.5676855895196, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: =1.14.13.7 [H]

Molecular weight: Translated: 26158; Mature: 26158

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPTVAHCQFVRDDGQPLDFQPGQFIQIHFDYADGTPTKRSYSLATIHDHALGPGEAVE
CCCCCCHHEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEE
IAVSFVPGGSATALFEGLEIGDQLQASGPYGRFCLQPGDHNQRYVLIATGTGVTPYRSML
EEEEEECCCCHHHHHHCCCCCCHHCCCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHH
PLLAEAIATRGVQVVLLQGARNPTELLYGDDFRAFADAHPQFRYVPCFSRDVPEQPHADV
HHHHHHHHHCCEEEEEEECCCCCCCEEECCCHHHHHCCCCCEEEECCCCCCCCCCCCCHH
RHGYVQQHLAEFVPDAAHDIAYLCGNPDMVDICLEALKAAGLPSAQIRREKYVSSTPPKV
HHHHHHHHHHHHCCCHHHHEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIAPTVAHCQFVRDDGQPLDFQPGQFIQIHFDYADGTPTKRSYSLATIHDHALGPGEAVE
CCCCCCHHEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEE
IAVSFVPGGSATALFEGLEIGDQLQASGPYGRFCLQPGDHNQRYVLIATGTGVTPYRSML
EEEEEECCCCHHHHHHCCCCCCHHCCCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHH
PLLAEAIATRGVQVVLLQGARNPTELLYGDDFRAFADAHPQFRYVPCFSRDVPEQPHADV
HHHHHHHHHCCEEEEEEECCCCCCCEEECCCHHHHHCCCCCEEEECCCCCCCCCCCCCHH
RHGYVQQHLAEFVPDAAHDIAYLCGNPDMVDICLEALKAAGLPSAQIRREKYVSSTPPKV
HHHHHHHHHHHHCCCHHHHEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12732969 [H]