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The map label for this gene is IS1086 [H]

Identifier: 84621819

GI number: 84621819

Start: 171426

End: 171860

Strand: Direct

Name: IS1086 [H]

Synonym: XOO_0162

Alternate gene names: 84621819

Gene position: 171426-171860 (Clockwise)

Preceding gene: 84621817

Following gene: 84621820

Centisome position: 3.47

GC content: 57.7

Gene sequence:

>435_bases
GTGGGCACGTTGGTGGAACGCAAGACGCGCTTTGTCGTGCTGTGCCGCATGGATGGCTGCACGGCCGCAGATGCGCTGGA
AGGGTTTACCCGGCAAATGAAGAAACTGCCGGCCTCAATGCGGACAAGTCTGACCTACGATCGCGGTACCGAGCTCACGT
GCTACGCCGAGCTGATGCAAGGATTGAACATCGACGTGTGGTTCGCTGATCCACATGCGCCGTGGCAGCGGGGAAGTAAC
GAGAACACCAACGGCCTGCTGCGCCAATTCCTGCCCAAGGGCGCCGACCTGTCCACTGTCAGCCAAGAGTATCTCAATCA
CATCGCACTGCTGATGAATACCCGCCCTCGTCAGACGCTCGGATGGAAGACACCAAGCGAGGCAATGGAGGAAGAAATCG
CAGCACTCAAATCACGTGTTGCACTTGAATCTTGA

Upstream 100 bases:

>100_bases
CGGTGGAGCGACGGTCCTACCATCGACCGGTCGGCTCTCACTGGCTGCGACTGTGCTGAAAGATCGTGCTAATCAGGAGG
GCGCATTCAATCGTTCTTGC

Downstream 100 bases:

>100_bases
GACTGCCCTCACCTTGCTGGACATGGTGGTTGCCGGGAAACAGCAGGGATCGCCCGGCGCGGAAAAACGCTTCCTGCAGC
AGCTCAGGGCACTGATTCCA

Product: ISXoo15 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MGTLVERKTRFVVLCRMDGCTAADALEGFTRQMKKLPASMRTSLTYDRGTELTCYAELMQGLNIDVWFADPHAPWQRGSN
ENTNGLLRQFLPKGADLSTVSQEYLNHIALLMNTRPRQTLGWKTPSEAMEEEIAALKSRVALES

Sequences:

>Translated_144_residues
MGTLVERKTRFVVLCRMDGCTAADALEGFTRQMKKLPASMRTSLTYDRGTELTCYAELMQGLNIDVWFADPHAPWQRGSN
ENTNGLLRQFLPKGADLSTVSQEYLNHIALLMNTRPRQTLGWKTPSEAMEEEIAALKSRVALES
>Mature_143_residues
GTLVERKTRFVVLCRMDGCTAADALEGFTRQMKKLPASMRTSLTYDRGTELTCYAELMQGLNIDVWFADPHAPWQRGSNE
NTNGLLRQFLPKGADLSTVSQEYLNHIALLMNTRPRQTLGWKTPSEAMEEEIAALKSRVALES

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=134, Percent_Identity=42.5373134328358, Blast_Score=129, Evalue=9e-32,
Organism=Escherichia coli, GI1790736, Length=134, Percent_Identity=42.5373134328358, Blast_Score=128, Evalue=1e-31,
Organism=Escherichia coli, GI1787671, Length=134, Percent_Identity=42.5373134328358, Blast_Score=128, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 16211; Mature: 16080

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLVERKTRFVVLCRMDGCTAADALEGFTRQMKKLPASMRTSLTYDRGTELTCYAELMQ
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHH
GLNIDVWFADPHAPWQRGSNENTNGLLRQFLPKGADLSTVSQEYLNHIALLMNTRPRQTL
CCCEEEEECCCCCHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GWKTPSEAMEEEIAALKSRVALES
CCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GTLVERKTRFVVLCRMDGCTAADALEGFTRQMKKLPASMRTSLTYDRGTELTCYAELMQ
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHH
GLNIDVWFADPHAPWQRGSNENTNGLLRQFLPKGADLSTVSQEYLNHIALLMNTRPRQTL
CCCEEEEECCCCCHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GWKTPSEAMEEEIAALKSRVALES
CCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1334071 [H]