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The map label for this gene is dosC [H]

Identifier: 84621724

GI number: 84621724

Start: 71996

End: 73624

Strand: Direct

Name: dosC [H]

Synonym: XOO_0067

Alternate gene names: 84621724

Gene position: 71996-73624 (Clockwise)

Preceding gene: 84621714

Following gene: 84621727

Centisome position: 1.46

GC content: 66.91

Gene sequence:

>1629_bases
ATGGCAGACCAGCCCGAATCCCCAAGCCGTCCACGTGGCGGGCTGCGCCGCCTGTTGCCCTGGAGCGGCGCGGACACTGC
GCGCACGGCCACGACTGTGCACACCGTGATCAACGGCGAGCGCGGCGGCCAGGGCCTGGCCGCGCATCAACTGGTACCGG
CCAAGGCCAAGTCCGGCGCACACGATCCCGCCGCCGCCACCGCATTGCTGATACGGCTGTTTTCGCATGCCGCGCATCCG
GAGGCACTGCTGCTCGCCTTCGCCGAAGGCATCACCAGCCTGCACGGCGAACTCGGCGACATGGGTGTGCGCCTGAGCGC
GGCCTATGCGGCGGGCGACTGGCCCGGCTACGGCCGTGCCTTGCGTCAGCTGATCGACAAGTACATCCGCACCATCGACA
TTGGCGATTCGCTGGCCGAAGGCCGCACCGAGGCCGAGCAACTGCGCGATCTGTTGCGCCACGCACTAGGCAATGCACTG
GCCACCCTGTTGCAGCGCAGCCCGGAGCTGGCCGAAGAGGCGCAGACGCTGGCATCGGCGCTGCGCCATTGGCGCCCGGG
CCACGAGTTGCTCACGCTGGAACAGCGCCTGCGCGAGCTCAGCCATCAGATCGGCCTGCGTGCCGAAGATGCCAGCGAAC
AGCAGAACCTGTTATTGGGCTTATTCGATCTGTTGCTGGAGAACGTCGGCGAACTCCTCGATGATCGCAGCTGGTTGCAA
GGCCAGATCTCGGTGGTGCGGCAGCTGATTTCCGGCCCGCTGGACGTCAGCTCCATCGAACAAGCCCGCGGCACCTTGCG
CGAGGTGATCTACAAACAAGGCCTGCTCAAACAGGGCATCGCCGAGTCCAAGACCGCCATGCGCGAAATGATGGTGTCCT
TCGTCGATCGGCTGGATGGCATGGCCACCAGCACCGGCGAATACCATGACCGCGTGGCCGGTTATTCGCAGGCGATTGGC
GAAGCACGCGGCATTCCCGACCTCAACCGGCTGCTGCAGGATCTGCTGCAGGACACCGCCGGTGTGCAGGCACAGGCGCT
GGCGGCGCGCGACGAGCTGCTTTCGGCACGGCGGCAGGTGGAAGATTGCGAACAACGCATCGCCTCGCTCGAGCAGGAGC
TCAAGGACGTGGCCGGCCTGGTGCGCGAAGACCAGCTCACGGGCGCGCTCAACCGACGCGGCTTTGAAGAACTGTTCCAG
CGCGAAGCGGTGCGCACCCAGCGCAGCGGCCAGCCGCTGTGCGTGGCGATGCTGGACCTGGACGATTTCCGCCGCCTCAA
CGAGACCCACGGCCACGCCGGCGGCGACGCCGCGCTGCGCCACACCGTGGACGTGGCCAAGGCCGTGCTGCGCACCACCG
ACGCCATCGCCCGCTTCGGCGGCGAAGAATTCGTCCTGCTGCTGCCGGATTCGACCATCTTCGAAGCCAGCGCGGCAGTG
ATCCGCCTGCAGCGCGCATTGTCGCAACGCTCGCTGCTGCATGACGACGTGCGCGTGTTCATCAGCTTCAGTGCCGGCGT
GGCACTCCGCGGGACCGACGAAACCCAGGACGACGTCATCCGCCGCGCCGACCGCGCCATGTACGACGCCAAGTCGGCCG
GCAAGAACCGGGTGATCAACGCCGATTAA

Upstream 100 bases:

>100_bases
CCTGCATGCTGCGGGTGCGCGCTGCGGCCCGGCTTGCTCAAGCACACCGTCGGCGCGCCGATAACGAGCGTTAGCAGAAG
ACTTTTCGAGACCCTGGCGC

Downstream 100 bases:

>100_bases
TCGCCCTGCCAACGCAGCGTCAGGTAATCCACCACCTGTGCGGTCTGGGGCTGGGCGAGAAATCCATGGCGAAACAGCAT
GCAGCCGCGTATCTGCGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MADQPESPSRPRGGLRRLLPWSGADTARTATTVHTVINGERGGQGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHP
EALLLAFAEGITSLHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNAL
ATLLQRSPELAEEAQTLASALRHWRPGHELLTLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQ
GQISVVRQLISGPLDVSSIEQARGTLREVIYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIG
EARGIPDLNRLLQDLLQDTAGVQAQALAARDELLSARRQVEDCEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQ
REAVRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVDVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAV
IRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDDVIRRADRAMYDAKSAGKNRVINAD

Sequences:

>Translated_542_residues
MADQPESPSRPRGGLRRLLPWSGADTARTATTVHTVINGERGGQGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHP
EALLLAFAEGITSLHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNAL
ATLLQRSPELAEEAQTLASALRHWRPGHELLTLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQ
GQISVVRQLISGPLDVSSIEQARGTLREVIYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIG
EARGIPDLNRLLQDLLQDTAGVQAQALAARDELLSARRQVEDCEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQ
REAVRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVDVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAV
IRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDDVIRRADRAMYDAKSAGKNRVINAD
>Mature_541_residues
ADQPESPSRPRGGLRRLLPWSGADTARTATTVHTVINGERGGQGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHPE
ALLLAFAEGITSLHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALA
TLLQRSPELAEEAQTLASALRHWRPGHELLTLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQG
QISVVRQLISGPLDVSSIEQARGTLREVIYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIGE
ARGIPDLNRLLQDLLQDTAGVQAQALAARDELLSARRQVEDCEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQR
EAVRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVDVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVI
RLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDDVIRRADRAMYDAKSAGKNRVINAD

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=231, Percent_Identity=29.004329004329, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=33.9080459770115, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=33.7423312883436, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=30.8571428571429, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786584, Length=155, Percent_Identity=32.258064516129, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=30.2857142857143, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=164, Percent_Identity=34.7560975609756, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788085, Length=187, Percent_Identity=32.620320855615, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59276; Mature: 59145

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQPESPSRPRGGLRRLLPWSGADTARTATTVHTVINGERGGQGLAAHQLVPAKAKSGA
CCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHCCCC
HDPAAATALLIRLFSHAAHPEALLLAFAEGITSLHGELGDMGVRLSAAYAAGDWPGYGRA
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCHHHHH
LRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEEAQTLASA
HHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRHWRPGHELLTLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQ
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GQISVVRQLISGPLDVSSIEQARGTLREVIYKQGLLKQGIAESKTAMREMMVSFVDRLDG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MATSTGEYHDRVAGYSQAIGEARGIPDLNRLLQDLLQDTAGVQAQALAARDELLSARRQV
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EDCEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPLCVAMLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCH
DDFRRLNETHGHAGGDAALRHTVDVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
IRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDDVIRRADRAMYDAKSAGKNRVIN
HHHHHHHHHHHHHHHHHHHHHEECCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
AD
CC
>Mature Secondary Structure 
ADQPESPSRPRGGLRRLLPWSGADTARTATTVHTVINGERGGQGLAAHQLVPAKAKSGA
CCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHCCCC
HDPAAATALLIRLFSHAAHPEALLLAFAEGITSLHGELGDMGVRLSAAYAAGDWPGYGRA
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCHHHHH
LRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEEAQTLASA
HHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRHWRPGHELLTLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQ
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GQISVVRQLISGPLDVSSIEQARGTLREVIYKQGLLKQGIAESKTAMREMMVSFVDRLDG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MATSTGEYHDRVAGYSQAIGEARGIPDLNRLLQDLLQDTAGVQAQALAARDELLSARRQV
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EDCEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPLCVAMLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCH
DDFRRLNETHGHAGGDAALRHTVDVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
IRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDDVIRRADRAMYDAKSAGKNRVIN
HHHHHHHHHHHHHHHHHHHHHEECCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
AD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]