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The map label for this gene is polB

Identifier: 84490361

GI number: 84490361

Start: 1762683

End: 1764155

Strand: Direct

Name: polB

Synonym: Msp_1584

Alternate gene names: 84490361

Gene position: 1762683-1764155 (Clockwise)

Preceding gene: 84490358

Following gene: 84490362

Centisome position: 99.73

GC content: 27.29

Gene sequence:

>1473_bases
ATGTCAAATGATATAATTAAAAAATTTCAAAAAGCACAACTGTTATTAAATAACAATGCTTATGCAGAAATCAAGGGAAA
TTCTGATTATGATTATCTAATTAATGAATTAATAGAATATATTGAAGAAAATGAAAATGATCAGTATATCATCACTACAC
AAACTATAAGAAATTATCAAGAACAAGAAAAAAGAAGAATACATGAATCAATAATTGAAAATCCAAAAACAAGAGAAATA
CGATCGAAACAAGAAACACCATACGAAGTAATACTTGATGTAACAAACAAATCATACACTGATGGAGATATTAATGATTT
AAATAAGTACTTCAATAGTAGATATGATAAATTAAAGAAAATTATACAAGAAAATCCTGAATTTAAGAAAATAAATGAAT
TAAAAACTACAAAAACAAACATTGATAATTTACAAGTTATTGGAATGATAAATAATATTAATAATACAAAAAATGGGCAT
AGAATAATAGAAATAGAAGATCCTACAGGTACTGGAAGTGTCATAATACTAAATGATAACGAAGATTTAATAAATAGTAG
TCAGACACTTGTAAAAGATGAAGTTATTGGAGTTAGTGGTAGTACTAATGGAACATTAATAAGAGCAGATGAAATTGTAC
ATCCAGGAATTCAAAGACATAAAATTATAGATAAAGACATGAATTTTTCAATAGTATTTATTTCAGATGTTCATATAGGT
AGTAAACAATTTGATGAAGATTCATTTAATAGATTCATTAAATGGTTAAATGGAAATTATGGAAATCAAGAGCAACAAGA
CCTTGCAAACGATGTTAAATACCTTGTTATTGGTGGAGACTTAGTTGATGGTATTGGAATCTATCCAAATCAAGAAAAGG
AATTAAAAATTAAAGATATATATGAACAATATGCTGAAGCTGCACGCCTATTAGGTGATATTACAGATATTCCTATAATA
TTATCTCCAGGTAACCATGATGCTACACGTCTTGCAGAACCTCAACCTGCATTAACTGAGAAATATGCTAAAGATTTATG
TAATCAGAAAAATATTGAAATGGTAAGTAATCCCTCAGTTGTTAACTTAGATAATATTAAAGTTCAAATATATCATGGTC
GAAGTTTTGATGATATTGTTATGGCTTTAAATAATTATACACATGCAGATACTGATAAAATAATGCAATTGTTACTGGAA
AAAAGACATCTTGCACCAATATATGGTGAACGTACACCACTTGCAAGTGAATTTGAGGATTATCTTGTTATTGATGATAT
TCCTGATGTATTACATACAGGACATGTCCATATTAATTCTCATACAAAATATAAAGGAGTTCATATGTTGAATTCTGGTA
CATTCCAAAAACAAACCGAGTTTCAGAAAATCTATAACATAGTACCTACATGTGCTCAAGTACCAGTATTAAATCATGGA
ACATTACAGTTATTGGATTTTACTAAAGAATAG

Upstream 100 bases:

>100_bases
GTTTATATTCTAATATTAATTTAAGTTAATTATAATATATATTATATATTATATTATTGAGATAAAAAAATATATAATTA
AAAAAAATGTAGCTGATCTA

Downstream 100 bases:

>100_bases
GAGTTAGATTTTATGGATAATAACATTATAGAAAATATAGTTAAAACAGCACATCATATTTATAATAAGGATATGATTAT
TGGAAAAGCAGGAAATATTA

Product: DNA polymerase II small subunit

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSNDIIKKFQKAQLLLNNNAYAEIKGNSDYDYLINELIEYIEENENDQYIITTQTIRNYQEQEKRRIHESIIENPKTREI
RSKQETPYEVILDVTNKSYTDGDINDLNKYFNSRYDKLKKIIQENPEFKKINELKTTKTNIDNLQVIGMINNINNTKNGH
RIIEIEDPTGTGSVIILNDNEDLINSSQTLVKDEVIGVSGSTNGTLIRADEIVHPGIQRHKIIDKDMNFSIVFISDVHIG
SKQFDEDSFNRFIKWLNGNYGNQEQQDLANDVKYLVIGGDLVDGIGIYPNQEKELKIKDIYEQYAEAARLLGDITDIPII
LSPGNHDATRLAEPQPALTEKYAKDLCNQKNIEMVSNPSVVNLDNIKVQIYHGRSFDDIVMALNNYTHADTDKIMQLLLE
KRHLAPIYGERTPLASEFEDYLVIDDIPDVLHTGHVHINSHTKYKGVHMLNSGTFQKQTEFQKIYNIVPTCAQVPVLNHG
TLQLLDFTKE

Sequences:

>Translated_490_residues
MSNDIIKKFQKAQLLLNNNAYAEIKGNSDYDYLINELIEYIEENENDQYIITTQTIRNYQEQEKRRIHESIIENPKTREI
RSKQETPYEVILDVTNKSYTDGDINDLNKYFNSRYDKLKKIIQENPEFKKINELKTTKTNIDNLQVIGMINNINNTKNGH
RIIEIEDPTGTGSVIILNDNEDLINSSQTLVKDEVIGVSGSTNGTLIRADEIVHPGIQRHKIIDKDMNFSIVFISDVHIG
SKQFDEDSFNRFIKWLNGNYGNQEQQDLANDVKYLVIGGDLVDGIGIYPNQEKELKIKDIYEQYAEAARLLGDITDIPII
LSPGNHDATRLAEPQPALTEKYAKDLCNQKNIEMVSNPSVVNLDNIKVQIYHGRSFDDIVMALNNYTHADTDKIMQLLLE
KRHLAPIYGERTPLASEFEDYLVIDDIPDVLHTGHVHINSHTKYKGVHMLNSGTFQKQTEFQKIYNIVPTCAQVPVLNHG
TLQLLDFTKE
>Mature_489_residues
SNDIIKKFQKAQLLLNNNAYAEIKGNSDYDYLINELIEYIEENENDQYIITTQTIRNYQEQEKRRIHESIIENPKTREIR
SKQETPYEVILDVTNKSYTDGDINDLNKYFNSRYDKLKKIIQENPEFKKINELKTTKTNIDNLQVIGMINNINNTKNGHR
IIEIEDPTGTGSVIILNDNEDLINSSQTLVKDEVIGVSGSTNGTLIRADEIVHPGIQRHKIIDKDMNFSIVFISDVHIGS
KQFDEDSFNRFIKWLNGNYGNQEQQDLANDVKYLVIGGDLVDGIGIYPNQEKELKIKDIYEQYAEAARLLGDITDIPIIL
SPGNHDATRLAEPQPALTEKYAKDLCNQKNIEMVSNPSVVNLDNIKVQIYHGRSFDDIVMALNNYTHADTDKIMQLLLEK
RHLAPIYGERTPLASEFEDYLVIDDIPDVLHTGHVHINSHTKYKGVHMLNSGTFQKQTEFQKIYNIVPTCAQVPVLNHGT
LQLLDFTKE

Specific function: Possesses two activities:a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase [H]

COG id: COG1311

COG function: function code L; Archaeal DNA polymerase II, small subunit/DNA polymerase delta, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase delta/II small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR012340
- InterPro:   IPR004365
- InterPro:   IPR011149 [H]

Pfam domain/function: PF00149 Metallophos; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 56183; Mature: 56052

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDIIKKFQKAQLLLNNNAYAEIKGNSDYDYLINELIEYIEENENDQYIITTQTIRNYQ
CCCHHHHHHHHHHEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
EQEKRRIHESIIENPKTREIRSKQETPYEVILDVTNKSYTDGDINDLNKYFNSRYDKLKK
HHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
IIQENPEFKKINELKTTKTNIDNLQVIGMINNINNTKNGHRIIEIEDPTGTGSVIILNDN
HHHCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEECCC
EDLINSSQTLVKDEVIGVSGSTNGTLIRADEIVHPGIQRHKIIDKDMNFSIVFISDVHIG
HHHHCCCHHHHHHHEEEECCCCCCEEEEECCHHCCCCHHHEEECCCCCEEEEEEEEEECC
SKQFDEDSFNRFIKWLNGNYGNQEQQDLANDVKYLVIGGDLVDGIGIYPNQEKELKIKDI
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHH
YEQYAEAARLLGDITDIPIILSPGNHDATRLAEPQPALTEKYAKDLCNQKNIEMVSNPSV
HHHHHHHHHHHHCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEECCCCE
VNLDNIKVQIYHGRSFDDIVMALNNYTHADTDKIMQLLLEKRHLAPIYGERTPLASEFED
EEECCEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCC
YLVIDDIPDVLHTGHVHINSHTKYKGVHMLNSGTFQKQTEFQKIYNIVPTCAQVPVLNHG
EEEECCCHHHHHCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCCC
TLQLLDFTKE
CEEEEEECCC
>Mature Secondary Structure 
SNDIIKKFQKAQLLLNNNAYAEIKGNSDYDYLINELIEYIEENENDQYIITTQTIRNYQ
CCHHHHHHHHHHEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
EQEKRRIHESIIENPKTREIRSKQETPYEVILDVTNKSYTDGDINDLNKYFNSRYDKLKK
HHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
IIQENPEFKKINELKTTKTNIDNLQVIGMINNINNTKNGHRIIEIEDPTGTGSVIILNDN
HHHCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEECCC
EDLINSSQTLVKDEVIGVSGSTNGTLIRADEIVHPGIQRHKIIDKDMNFSIVFISDVHIG
HHHHCCCHHHHHHHEEEECCCCCCEEEEECCHHCCCCHHHEEECCCCCEEEEEEEEEECC
SKQFDEDSFNRFIKWLNGNYGNQEQQDLANDVKYLVIGGDLVDGIGIYPNQEKELKIKDI
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHH
YEQYAEAARLLGDITDIPIILSPGNHDATRLAEPQPALTEKYAKDLCNQKNIEMVSNPSV
HHHHHHHHHHHHCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEECCCCE
VNLDNIKVQIYHGRSFDDIVMALNNYTHADTDKIMQLLLEKRHLAPIYGERTPLASEFED
EEECCEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCC
YLVIDDIPDVLHTGHVHINSHTKYKGVHMLNSGTFQKQTEFQKIYNIVPTCAQVPVLNHG
EEEECCCHHHHHCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCCC
TLQLLDFTKE
CEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]