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The map label for this gene is ksgA

Identifier: 84490322

GI number: 84490322

Start: 1722908

End: 1723723

Strand: Direct

Name: ksgA

Synonym: Msp_1544

Alternate gene names: 84490322

Gene position: 1722908-1723723 (Clockwise)

Preceding gene: 84490321

Following gene: 84490323

Centisome position: 97.48

GC content: 26.72

Gene sequence:

>816_bases
ATGTCAAATACAAAAGAGATACTAGAAAAATATAATATTAAACTAGATACAAATAAGAGTCAAAACTATCTAATTGATGA
TAATAAACTTAACATAATTCTAGAAAATGCAGATATTCAAGATAATGAAACAATTCTTGAAATTGGTGCTGGAATTGGAA
CACTAACACTTCCTATGGCTAAAAAAGCTAAAAAAGTAATAGCCATAGAAAAGGATCCTATTATAGTTGACATACTAAAA
CAACAAATCATAAAAGAAAAACTAACAAACATAGAAATAATCAAAGATGATGCTCTAAAAGTAGACTTTCCAAAGTTTGA
CAAAGTAGTTTCAAATCTACCATACCAGATATCTTCACCAGTGACATTCAAATTACTAGAATATCCCTTTAAAAAAGCTA
TTTTAATGTATCAACTAGAATTTGCAAAAAGAATGCAGGCAAAACCAGATACGCATGAATATTCTAGATTATCAGTTGCC
CTATCCTATAGAGCAGATACAAAAATTATAGATACTTTACCTCCTGAAGCATTCATACCAAAACCTAAAATTAAAAGTGC
AGTAATAGAATTAATACCTAAAAATAACAAACCAATTGATAAACTATTAGATAATACAATACGAGCCTTATTCCAACATA
GAAATAAAAAAGCAAAAAAAGCATTAATTCAATCAGCACATGAATTAGGTGTTGATAAAAAGGTATTAAAACAAAAATTA
AGTAATGTTACTAATGACTTATTTGAAGAAAAAGTATTTAAACTAACTCCAACTCAAATAAAAGAAATATCATTAATATT
AGAGGGAAATCTATGA

Upstream 100 bases:

>100_bases
AACCTTCCCCTACTTTATTCTTTCAATAACCACCAAACAAATAAACTTATTTTAATTAATTACTAGTTAATCTAAAAACC
ATTAATAGGACAATAAAACT

Downstream 100 bases:

>100_bases
AATATGAAAATATTAAGTATAATGAATGTGATGAAGTATATCCTCCAGCAGAGGACACTTTTTTATTAATTGATAATCTT
ATTGTTAAAAATAACGATTC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSNTKEILEKYNIKLDTNKSQNYLIDDNKLNIILENADIQDNETILEIGAGIGTLTLPMAKKAKKVIAIEKDPIIVDILK
QQIIKEKLTNIEIIKDDALKVDFPKFDKVVSNLPYQISSPVTFKLLEYPFKKAILMYQLEFAKRMQAKPDTHEYSRLSVA
LSYRADTKIIDTLPPEAFIPKPKIKSAVIELIPKNNKPIDKLLDNTIRALFQHRNKKAKKALIQSAHELGVDKKVLKQKL
SNVTNDLFEEKVFKLTPTQIKEISLILEGNL

Sequences:

>Translated_271_residues
MSNTKEILEKYNIKLDTNKSQNYLIDDNKLNIILENADIQDNETILEIGAGIGTLTLPMAKKAKKVIAIEKDPIIVDILK
QQIIKEKLTNIEIIKDDALKVDFPKFDKVVSNLPYQISSPVTFKLLEYPFKKAILMYQLEFAKRMQAKPDTHEYSRLSVA
LSYRADTKIIDTLPPEAFIPKPKIKSAVIELIPKNNKPIDKLLDNTIRALFQHRNKKAKKALIQSAHELGVDKKVLKQKL
SNVTNDLFEEKVFKLTPTQIKEISLILEGNL
>Mature_270_residues
SNTKEILEKYNIKLDTNKSQNYLIDDNKLNIILENADIQDNETILEIGAGIGTLTLPMAKKAKKVIAIEKDPIIVDILKQ
QIIKEKLTNIEIIKDDALKVDFPKFDKVVSNLPYQISSPVTFKLLEYPFKKAILMYQLEFAKRMQAKPDTHEYSRLSVAL
SYRADTKIIDTLPPEAFIPKPKIKSAVIELIPKNNKPIDKLLDNTIRALFQHRNKKAKKALIQSAHELGVDKKVLKQKLS
NVTNDLFEEKVFKLTPTQIKEISLILEGNL

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=220, Percent_Identity=36.3636363636364, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI156415992, Length=252, Percent_Identity=25.7936507936508, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1786236, Length=243, Percent_Identity=28.8065843621399, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=210, Percent_Identity=37.1428571428571, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI25141369, Length=252, Percent_Identity=23.8095238095238, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=34.9056603773585, Blast_Score=118, Evalue=8e-28,
Organism=Drosophila melanogaster, GI21358017, Length=214, Percent_Identity=35.5140186915888, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21357273, Length=248, Percent_Identity=26.2096774193548, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_METST (Q2NE42)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448554.1
- ProteinModelPortal:   Q2NE42
- SMR:   Q2NE42
- STRING:   Q2NE42
- GeneID:   3854849
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1544
- NMPDR:   fig|339860.6.peg.1477
- eggNOG:   arNOG05894
- HOGENOM:   HBG319664
- OMA:   LAVLMYQ
- PhylomeDB:   Q2NE42
- ProtClustDB:   PRK14896
- BioCyc:   MSTA339860:MSP_1544-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30933; Mature: 30802

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 22-22 BINDING 24-24 BINDING 49-49 BINDING 70-70 BINDING 97-97 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTKEILEKYNIKLDTNKSQNYLIDDNKLNIILENADIQDNETILEIGAGIGTLTLPMA
CCCHHHHHHHHCCEEECCCCCCEEEECCCEEEEEECCCCCCCCEEEEECCCCCEEEHHHH
KKAKKVIAIEKDPIIVDILKQQIIKEKLTNIEIIKDDALKVDFPKFDKVVSNLPYQISSP
HHCCCEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCCEEECCCHHHHHHHCCCCCCCCC
VTFKLLEYPFKKAILMYQLEFAKRMQAKPDTHEYSRLSVALSYRADTKIIDTLPPEAFIP
EEHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEECCCCEEEECCCCCCCCC
KPKIKSAVIELIPKNNKPIDKLLDNTIRALFQHRNKKAKKALIQSAHELGVDKKVLKQKL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHH
SNVTNDLFEEKVFKLTPTQIKEISLILEGNL
HHHHHHHHHHHHHHCCCHHHEEEEEEEECCC
>Mature Secondary Structure 
SNTKEILEKYNIKLDTNKSQNYLIDDNKLNIILENADIQDNETILEIGAGIGTLTLPMA
CCHHHHHHHHCCEEECCCCCCEEEECCCEEEEEECCCCCCCCEEEEECCCCCEEEHHHH
KKAKKVIAIEKDPIIVDILKQQIIKEKLTNIEIIKDDALKVDFPKFDKVVSNLPYQISSP
HHCCCEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCCEEECCCHHHHHHHCCCCCCCCC
VTFKLLEYPFKKAILMYQLEFAKRMQAKPDTHEYSRLSVALSYRADTKIIDTLPPEAFIP
EEHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEECCCCEEEECCCCCCCCC
KPKIKSAVIELIPKNNKPIDKLLDNTIRALFQHRNKKAKKALIQSAHELGVDKKVLKQKL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHH
SNVTNDLFEEKVFKLTPTQIKEISLILEGNL
HHHHHHHHHHHHHHCCCHHHEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA