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The map label for this gene is gatD

Identifier: 84490193

GI number: 84490193

Start: 1584895

End: 1586211

Strand: Direct

Name: gatD

Synonym: Msp_1411

Alternate gene names: 84490193

Gene position: 1584895-1586211 (Clockwise)

Preceding gene: 84490192

Following gene: 84490194

Centisome position: 89.67

GC content: 31.36

Gene sequence:

>1317_bases
TTGGCATATGAAGAATATCTTAAAGATTTTCTAGAAATATCAAATATTACTGTTGGAGACACAGTAAAAGTAACAAAACC
CCATATAGAACATGAAGGAATGTTACTTGAAAAACCAGACTATTCAAATGAAAACACTATAATACTAAAATTAGATAGTG
GATACAATATTGGTATTGATATAAAAGATGCAAAAATAGAAAAAATAAGTGAAGGTAAAAAACCTCAAATAGAATTAGAT
CCTGTTGATAAAAAAATCTCTGATAAAAAGAAAAATTTATCAATACTTTCAACTGGAGGTACAGTTGCAAGTGTAATTGA
CTATAAAACAGGAGCAGTACATCCAGCATTTACAGCAGATGACCTGCTCAGAGCAACACCAGAACTTGTTGATTATGCAA
ATATAAATGCTAAAGCTATATTCAATATTCTAAGTGAAAATATGACACCAGAATATTGGAAAAAAACAGCAACCACAATC
TATGATGAAATAAATAATGGAGCAGATGGAATAATCATAGCTCATGGAACAGATACAATGCATTATACTGCATCTGCATT
AAGTTTCATGATAGACTCTCCCGTACCTATTGTTTTAACAGGAGCACAACGTAGCTCAGATAGACCATCATCTGATGCAT
TTACAAATCTTATGGCATCAGTTAATGCAGCAAAATCTGATATTGCAGAAGTTACAATATGTATGCATGGAACTGAAGAT
GATTCCTACTGTGATCTACACAGAGGTACAAGAGCACGTAAAATGCACACATCACGTCGTGACACATTTACAAGTATAAA
TATGAATCCACTTGCAAGAATTGAAAATAACAAAGTAACAATAAATGATACACAAGTAAAATACACTAAAAGAAATGAAA
AAGAACTATCCCTAAATACTAATCTTGCAGATAAAGTAGCATTAATTAAAATGTATCCTGGAATTAGTCCTGAAATTATA
GACATCTATGTAGATAAAAACTATGATGGAATTGTAATTGAAGGAACTGGTCTTGGTCATTGTAGTGATGATGTAATAAC
AAGTATTACAAGAGCAACAAGTGAAGACATTCCTGTTGTCATGACTTCCCAATGTTTATTTGGACGTACAAATATGAATG
TGTATAGTAGTGGTCGTAGATTACTACAAAACAATGTAATTGGTGTGGGAGATATGTTACCTGAAACAGCTTATACAAAA
CTACTATGGGCTGCAGGACAGACAGATAATGTATCAGAAATATATGAAATAATGAAAACAAATCTTAAAGGAGAAATGGA
TACAACATTATCCCAAAATTACTTTATAAAAAACTAA

Upstream 100 bases:

>100_bases
TAGTACTTAAATTAAGAAAAATATGAAAAAATACAACTTATACTATAATTTTTATTCAAATAATAACTAAAAAACTATTT
TTCACAAGGAGGAAAAGAAT

Downstream 100 bases:

>100_bases
AGGAGTGCTGAAAAATTGTCTGAAGATAAATTTAATTATAAAGAATTAGGATTAATGATGGGATTAGAAATTCATCAACA
ACTAGATAGTAAAACTAAAC

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MAYEEYLKDFLEISNITVGDTVKVTKPHIEHEGMLLEKPDYSNENTIILKLDSGYNIGIDIKDAKIEKISEGKKPQIELD
PVDKKISDKKKNLSILSTGGTVASVIDYKTGAVHPAFTADDLLRATPELVDYANINAKAIFNILSENMTPEYWKKTATTI
YDEINNGADGIIIAHGTDTMHYTASALSFMIDSPVPIVLTGAQRSSDRPSSDAFTNLMASVNAAKSDIAEVTICMHGTED
DSYCDLHRGTRARKMHTSRRDTFTSINMNPLARIENNKVTINDTQVKYTKRNEKELSLNTNLADKVALIKMYPGISPEII
DIYVDKNYDGIVIEGTGLGHCSDDVITSITRATSEDIPVVMTSQCLFGRTNMNVYSSGRRLLQNNVIGVGDMLPETAYTK
LLWAAGQTDNVSEIYEIMKTNLKGEMDTTLSQNYFIKN

Sequences:

>Translated_438_residues
MAYEEYLKDFLEISNITVGDTVKVTKPHIEHEGMLLEKPDYSNENTIILKLDSGYNIGIDIKDAKIEKISEGKKPQIELD
PVDKKISDKKKNLSILSTGGTVASVIDYKTGAVHPAFTADDLLRATPELVDYANINAKAIFNILSENMTPEYWKKTATTI
YDEINNGADGIIIAHGTDTMHYTASALSFMIDSPVPIVLTGAQRSSDRPSSDAFTNLMASVNAAKSDIAEVTICMHGTED
DSYCDLHRGTRARKMHTSRRDTFTSINMNPLARIENNKVTINDTQVKYTKRNEKELSLNTNLADKVALIKMYPGISPEII
DIYVDKNYDGIVIEGTGLGHCSDDVITSITRATSEDIPVVMTSQCLFGRTNMNVYSSGRRLLQNNVIGVGDMLPETAYTK
LLWAAGQTDNVSEIYEIMKTNLKGEMDTTLSQNYFIKN
>Mature_437_residues
AYEEYLKDFLEISNITVGDTVKVTKPHIEHEGMLLEKPDYSNENTIILKLDSGYNIGIDIKDAKIEKISEGKKPQIELDP
VDKKISDKKKNLSILSTGGTVASVIDYKTGAVHPAFTADDLLRATPELVDYANINAKAIFNILSENMTPEYWKKTATTIY
DEINNGADGIIIAHGTDTMHYTASALSFMIDSPVPIVLTGAQRSSDRPSSDAFTNLMASVNAAKSDIAEVTICMHGTEDD
SYCDLHRGTRARKMHTSRRDTFTSINMNPLARIENNKVTINDTQVKYTKRNEKELSLNTNLADKVALIKMYPGISPEIID
IYVDKNYDGIVIEGTGLGHCSDDVITSITRATSEDIPVVMTSQCLFGRTNMNVYSSGRRLLQNNVIGVGDMLPETAYTKL
LWAAGQTDNVSEIYEIMKTNLKGEMDTTLSQNYFIKN

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=375, Percent_Identity=27.4666666666667, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1788065, Length=356, Percent_Identity=30.0561797752809, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789327, Length=300, Percent_Identity=29.6666666666667, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193207372, Length=288, Percent_Identity=30.5555555555556, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17558280, Length=288, Percent_Identity=30.5555555555556, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI193207374, Length=288, Percent_Identity=30.5555555555556, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17558278, Length=288, Percent_Identity=30.5555555555556, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6320527, Length=332, Percent_Identity=25.6024096385542, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323189, Length=272, Percent_Identity=28.6764705882353, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323187, Length=272, Percent_Identity=28.6764705882353, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323186, Length=272, Percent_Identity=28.6764705882353, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323184, Length=272, Percent_Identity=28.6764705882353, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24645530, Length=282, Percent_Identity=30.8510638297872, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24639609, Length=288, Percent_Identity=28.4722222222222, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_METST (Q2NEH1)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448425.1
- ProteinModelPortal:   Q2NEH1
- SMR:   Q2NEH1
- STRING:   Q2NEH1
- GeneID:   3854887
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1411
- NMPDR:   fig|339860.6.peg.1349
- eggNOG:   arNOG04527
- HOGENOM:   HBG497495
- OMA:   KMKSGYN
- PhylomeDB:   Q2NEH1
- ProtClustDB:   PRK04183
- BioCyc:   MSTA339860:MSP_1411-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 48555; Mature: 48423

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 101-101 ACT_SITE 177-177 ACT_SITE 178-178 ACT_SITE 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYEEYLKDFLEISNITVGDTVKVTKPHIEHEGMLLEKPDYSNENTIILKLDSGYNIGID
CCHHHHHHHHHHHCCEEECCEEEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCEEEEE
IKDAKIEKISEGKKPQIELDPVDKKISDKKKNLSILSTGGTVASVIDYKTGAVHPAFTAD
ECCCHHHHHHCCCCCEEEECCCCCHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCHH
DLLRATPELVDYANINAKAIFNILSENMTPEYWKKTATTIYDEINNGADGIIIAHGTDTM
HHHHCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
HYTASALSFMIDSPVPIVLTGAQRSSDRPSSDAFTNLMASVNAAKSDIAEVTICMHGTED
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
DSYCDLHRGTRARKMHTSRRDTFTSINMNPLARIENNKVTINDTQVKYTKRNEKELSLNT
CCEEHHHCCCHHHHHHCCCCCCEEEECCCCEEEECCCEEEEECCEEEEECCCCCEEEECC
NLADKVALIKMYPGISPEIIDIYVDKNYDGIVIEGTGLGHCSDDVITSITRATSEDIPVV
CCHHHEEEEEECCCCCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEE
MTSQCLFGRTNMNVYSSGRRLLQNNVIGVGDMLPETAYTKLLWAAGQTDNVSEIYEIMKT
EECCEEECCCCCCHHHHHHHHHHCCCEECHHHCCHHHHHHHEEECCCCCCHHHHHHHHHH
NLKGEMDTTLSQNYFIKN
CCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
AYEEYLKDFLEISNITVGDTVKVTKPHIEHEGMLLEKPDYSNENTIILKLDSGYNIGID
CHHHHHHHHHHHCCEEECCEEEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCEEEEE
IKDAKIEKISEGKKPQIELDPVDKKISDKKKNLSILSTGGTVASVIDYKTGAVHPAFTAD
ECCCHHHHHHCCCCCEEEECCCCCHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCHH
DLLRATPELVDYANINAKAIFNILSENMTPEYWKKTATTIYDEINNGADGIIIAHGTDTM
HHHHCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
HYTASALSFMIDSPVPIVLTGAQRSSDRPSSDAFTNLMASVNAAKSDIAEVTICMHGTED
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
DSYCDLHRGTRARKMHTSRRDTFTSINMNPLARIENNKVTINDTQVKYTKRNEKELSLNT
CCEEHHHCCCHHHHHHCCCCCCEEEECCCCEEEECCCEEEEECCEEEEECCCCCEEEECC
NLADKVALIKMYPGISPEIIDIYVDKNYDGIVIEGTGLGHCSDDVITSITRATSEDIPVV
CCHHHEEEEEECCCCCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEE
MTSQCLFGRTNMNVYSSGRRLLQNNVIGVGDMLPETAYTKLLWAAGQTDNVSEIYEIMKT
EECCEEECCCCCCHHHHHHHHHHCCCEECHHHCCHHHHHHHEEECCCCCCHHHHHHHHHH
NLKGEMDTTLSQNYFIKN
CCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA