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The map label for this gene is hisA [C]

Identifier: 84490045

GI number: 84490045

Start: 1422756

End: 1423475

Strand: Direct

Name: hisA [C]

Synonym: Msp_1257

Alternate gene names: 84490045

Gene position: 1422756-1423475 (Clockwise)

Preceding gene: 84490044

Following gene: 84490046

Centisome position: 80.5

GC content: 22.92

Gene sequence:

>720_bases
TTGATTTTTATGATTATTCCTGTAATTGACATTATGCACAATGAATGTGTTAGTGGAAAATCTGGTAGACGAAGTACTTA
TAAAAAATTAAGAAGTATTTATGGTGATAATCCATTAGATATTGCAACTGCTCTAAAAGAAGATGGTGCTCAACTAATAT
ATATTGCTGATTTAGATAAAATTGAAAGGATAGATGATAATAACAATATAATATCTTCTATAAATAATATTATTCCTGTA
ATGTTAGATAATGGTATTTCATCTATTGAAGATATTGAAAATAATAAAAATATATGTAGTTACAATATATTAGCTACTGA
AACTATGACAAGTCTTGACAACATAGAAGAAATATTTAAAAAGTATGATAGTAATAAGCTAGTTGTTAGTATAGATATTA
AAAATAATGAGCTTCTTGTTAATAATAAGGATATTGATCTTGAGGATGTAATTTATTTAATTAATAAGTATAAACCAAAA
TATACTATAATTCTTAATATTTCATATGTTGGAACTAAAAAAGGTGTAAATACTAATATAATAGAAGAAATTACTAAAAA
AATACCCCACACAAAATGTTGTATAGCAGGTGGTATTAGAAATAGTGATATTGAAACATACACTAAAAAAAATATTAACA
ACTTCCTTATTGGAACATTACTTCATGAAGGTAATCTTAGTTATAAATTATATATGGAAAGTAAAAAAAATGAAAAATAA

Upstream 100 bases:

>100_bases
AGATAGTGATATGAAACTACCTGAAAATTTAGCAAATAGTGCTATTGATGTGGCCCTAGAAGCAGTTGATGAATATGTTG
AAAATAGAAACAATTAAGTT

Downstream 100 bases:

>100_bases
AATACTTACTATAGGTCCAATTACAAAAGATATTATAAAAACACCAACACAAACATATATTCAAACAGGCGGTGCATCAT
ATTATCAGATGTGGACATTA

Product: hypothetical protein

Products: 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-; $phosphoribosyl)imidazole-4-carboxamide

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIFMIIPVIDIMHNECVSGKSGRRSTYKKLRSIYGDNPLDIATALKEDGAQLIYIADLDKIERIDDNNNIISSINNIIPV
MLDNGISSIEDIENNKNICSYNILATETMTSLDNIEEIFKKYDSNKLVVSIDIKNNELLVNNKDIDLEDVIYLINKYKPK
YTIILNISYVGTKKGVNTNIIEEITKKIPHTKCCIAGGIRNSDIETYTKKNINNFLIGTLLHEGNLSYKLYMESKKNEK

Sequences:

>Translated_239_residues
MIFMIIPVIDIMHNECVSGKSGRRSTYKKLRSIYGDNPLDIATALKEDGAQLIYIADLDKIERIDDNNNIISSINNIIPV
MLDNGISSIEDIENNKNICSYNILATETMTSLDNIEEIFKKYDSNKLVVSIDIKNNELLVNNKDIDLEDVIYLINKYKPK
YTIILNISYVGTKKGVNTNIIEEITKKIPHTKCCIAGGIRNSDIETYTKKNINNFLIGTLLHEGNLSYKLYMESKKNEK
>Mature_239_residues
MIFMIIPVIDIMHNECVSGKSGRRSTYKKLRSIYGDNPLDIATALKEDGAQLIYIADLDKIERIDDNNNIISSINNIIPV
MLDNGISSIEDIENNKNICSYNILATETMTSLDNIEEIFKKYDSNKLVVSIDIKNNELLVNNKDIDLEDVIYLINKYKPK
YTIILNISYVGTKKGVNTNIIEEITKKIPHTKCCIAGGIRNSDIETYTKKNINNFLIGTLLHEGNLSYKLYMESKKNEK

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG1411

COG function: function code R; Uncharacterized protein related to proFAR isomerase (HisA)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI87082028, Length=253, Percent_Identity=24.5059288537549, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004650
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 5.3.1.16

Molecular weight: Translated: 27176; Mature: 27176

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFMIIPVIDIMHNECVSGKSGRRSTYKKLRSIYGDNPLDIATALKEDGAQLIYIADLDK
CEEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCHH
IERIDDNNNIISSINNIIPVMLDNGISSIEDIENNKNICSYNILATETMTSLDNIEEIFK
HHHCCCCCHHHHHHHHHEEEEECCCCHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHH
KYDSNKLVVSIDIKNNELLVNNKDIDLEDVIYLINKYKPKYTIILNISYVGTKKGVNTNI
HCCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHH
IEEITKKIPHTKCCIAGGIRNSDIETYTKKNINNFLIGTLLHEGNLSYKLYMESKKNEK
HHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCHHEEEEEEECCCCEEEEEEECCCCCC
>Mature Secondary Structure
MIFMIIPVIDIMHNECVSGKSGRRSTYKKLRSIYGDNPLDIATALKEDGAQLIYIADLDK
CEEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCHH
IERIDDNNNIISSINNIIPVMLDNGISSIEDIENNKNICSYNILATETMTSLDNIEEIFK
HHHCCCCCHHHHHHHHHEEEEECCCCHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHH
KYDSNKLVVSIDIKNNELLVNNKDIDLEDVIYLINKYKPKYTIILNISYVGTKKGVNTNI
HCCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHH
IEEITKKIPHTKCCIAGGIRNSDIETYTKKNINNFLIGTLLHEGNLSYKLYMESKKNEK
HHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCHHEEEEEEECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 1-(5-phosphoribosyl)-5-[(5-; $phosphoribosylamino)methylideneamino]imidazole-4-carboxamide

Specific reaction: 1-(5-phosphoribosyl)-5-[(5- $phosphoribosylamino)methylideneamino]imidazole-4-carboxamide = 5- $[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5- $phosphoribosyl)imidazole-4-carboxamide

General reaction: Isomerases; Intramolecular Oxidoreductases; Interconverting Aldoses and Ketoses [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]