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The map label for this gene is gmd [H]

Identifier: 84489789

GI number: 84489789

Start: 1138753

End: 1139985

Strand: Direct

Name: gmd [H]

Synonym: Msp_0990

Alternate gene names: 84489789

Gene position: 1138753-1139985 (Clockwise)

Preceding gene: 84489786

Following gene: 84489790

Centisome position: 64.43

GC content: 29.28

Gene sequence:

>1233_bases
ATGAAATGGCAAGATAAAAATGTATTAATTACGGGAATAAGTGGATTTGTTGGTGCATATTTAGCAGAAAGACTTATAAA
AGAAGGAAGTAATGTTTATGGATTAGTTAGAAGAAGAACTGATGGATCCATTCCTACTAATATTAAAAACCATGGAATTG
AAAATGATTTTACCCAGATAACTGGTGATTTAACAGATATTAGTTCTGTTGCCAATGCTATTGATAAATCAGAACCAGAT
TATATCTTTCATTTAGCAGCACAATCCTTTGTACCACAATCATTTGATAATCCTACAGACACTGAACAAAATAATTCACA
AGGAACTAATAATTTACTTGAAGCAATGCGAATAAAAGATTGTGATGCACGTATGGTATTTGCTGGTTCAAGTGAAGAAT
ATGGTCTTGTAATCTCCTCAAAAGATCAATATAATGCTGCACTTAAACAATATGGTTCCATATTTCCAGATATTAAAGAA
GGAATTACACATGAATTACCAATATCTGAAACAAATCCTCTACGTCCAATGAGTCCTTATGCTGTTTCAAAAGTTTATGG
TGATTTCTTAACAAGAAATTATTATCATTCATATGGATTAGATACTGTTGTATCAAGAGCATTTAATCATGAAGGTGCTG
GTCGTGGAAAAATGTTTGTAACAAGTGTAGTTACAAATCAAGTAATACAACTAAAATTAGGCCTAACTGATAAAATAACA
ATAGGAAATGTTAATGCATTTAGAGACTGGACACATATTGAAGATATTATAAATGGATACCTTCTTCTTGCAAAAAAAGG
TAATAGTGGGGATGTATACAATCAAGGTTCCATGAGAACAAATTCAATACTAAGTTATATTCTACTAAGTATTGAACAAA
TCGGTCAAAAAATAGAATCCATAAGTACATTAAATGGAGATAAAGTTATAACTAATCCAACAGAACTTAACTCTGACAAG
TATTATGGAGTTTCATTTGATAAAACAAAAGTAGATTCAATGATACTAGATGATGAAATTGAATATACAATAGAAGATAA
GGGAATAAATGTAAAAACTGATACAGATAATATTAAAATTGAATTCAATCCTGCAAGATTTAGACCAGCAGAAGTACCTA
TATTATTCTCAAATACAGCTAAAATACAAAAGTTAGGTGCTAAAACTGAACATTCAGTATCTGATATTATAAAAGATCAA
TTAAACTACTTTATAAAAGAAGAAAATAGATAA

Upstream 100 bases:

>100_bases
TTGTGTGATTTTTTATTTATTATTTATGAAATCATATAAACTATAAAAAATATATTAATATTAAAAGAATATTAAAACTT
TTTAAATAAGAGATGTAACT

Downstream 100 bases:

>100_bases
AATATTTTCTTTCATACACACTTTTTTTGGGAGAGATATATATGAACATATGTATAGTTACTGAATATTTTCCACATAGT
GAAAATCTTGAAATAAAAGG

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKWQDKNVLITGISGFVGAYLAERLIKEGSNVYGLVRRRTDGSIPTNIKNHGIENDFTQITGDLTDISSVANAIDKSEPD
YIFHLAAQSFVPQSFDNPTDTEQNNSQGTNNLLEAMRIKDCDARMVFAGSSEEYGLVISSKDQYNAALKQYGSIFPDIKE
GITHELPISETNPLRPMSPYAVSKVYGDFLTRNYYHSYGLDTVVSRAFNHEGAGRGKMFVTSVVTNQVIQLKLGLTDKIT
IGNVNAFRDWTHIEDIINGYLLLAKKGNSGDVYNQGSMRTNSILSYILLSIEQIGQKIESISTLNGDKVITNPTELNSDK
YYGVSFDKTKVDSMILDDEIEYTIEDKGINVKTDTDNIKIEFNPARFRPAEVPILFSNTAKIQKLGAKTEHSVSDIIKDQ
LNYFIKEENR

Sequences:

>Translated_410_residues
MKWQDKNVLITGISGFVGAYLAERLIKEGSNVYGLVRRRTDGSIPTNIKNHGIENDFTQITGDLTDISSVANAIDKSEPD
YIFHLAAQSFVPQSFDNPTDTEQNNSQGTNNLLEAMRIKDCDARMVFAGSSEEYGLVISSKDQYNAALKQYGSIFPDIKE
GITHELPISETNPLRPMSPYAVSKVYGDFLTRNYYHSYGLDTVVSRAFNHEGAGRGKMFVTSVVTNQVIQLKLGLTDKIT
IGNVNAFRDWTHIEDIINGYLLLAKKGNSGDVYNQGSMRTNSILSYILLSIEQIGQKIESISTLNGDKVITNPTELNSDK
YYGVSFDKTKVDSMILDDEIEYTIEDKGINVKTDTDNIKIEFNPARFRPAEVPILFSNTAKIQKLGAKTEHSVSDIIKDQ
LNYFIKEENR
>Mature_410_residues
MKWQDKNVLITGISGFVGAYLAERLIKEGSNVYGLVRRRTDGSIPTNIKNHGIENDFTQITGDLTDISSVANAIDKSEPD
YIFHLAAQSFVPQSFDNPTDTEQNNSQGTNNLLEAMRIKDCDARMVFAGSSEEYGLVISSKDQYNAALKQYGSIFPDIKE
GITHELPISETNPLRPMSPYAVSKVYGDFLTRNYYHSYGLDTVVSRAFNHEGAGRGKMFVTSVVTNQVIQLKLGLTDKIT
IGNVNAFRDWTHIEDIINGYLLLAKKGNSGDVYNQGSMRTNSILSYILLSIEQIGQKIESISTLNGDKVITNPTELNSDK
YYGVSFDKTKVDSMILDDEIEYTIEDKGINVKTDTDNIKIEFNPARFRPAEVPILFSNTAKIQKLGAKTEHSVSDIIKDQ
LNYFIKEENR

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=301, Percent_Identity=32.5581395348837, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI7657641, Length=291, Percent_Identity=24.7422680412371, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788366, Length=413, Percent_Identity=28.8135593220339, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI48994969, Length=279, Percent_Identity=27.2401433691756, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788353, Length=275, Percent_Identity=25.8181818181818, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI133901788, Length=406, Percent_Identity=29.5566502463054, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI133901786, Length=406, Percent_Identity=29.5566502463054, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17539422, Length=406, Percent_Identity=29.5566502463054, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17539424, Length=406, Percent_Identity=29.5566502463054, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI133901790, Length=406, Percent_Identity=29.5566502463054, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17507723, Length=407, Percent_Identity=28.2555282555283, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17568069, Length=279, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24158427, Length=392, Percent_Identity=27.2959183673469, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 45837; Mature: 45837

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWQDKNVLITGISGFVGAYLAERLIKEGSNVYGLVRRRTDGSIPTNIKNHGIENDFTQI
CCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCCCCHHHH
TGDLTDISSVANAIDKSEPDYIFHLAAQSFVPQSFDNPTDTEQNNSQGTNNLLEAMRIKD
HCCHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
CDARMVFAGSSEEYGLVISSKDQYNAALKQYGSIFPDIKEGITHELPISETNPLRPMSPY
CCCEEEEECCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCH
AVSKVYGDFLTRNYYHSYGLDTVVSRAFNHEGAGRGKMFVTSVVTNQVIQLKLGLTDKIT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEHHHHHCCEEEEEECCCCEEE
IGNVNAFRDWTHIEDIINGYLLLAKKGNSGDVYNQGSMRTNSILSYILLSIEQIGQKIES
ECCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ISTLNGDKVITNPTELNSDKYYGVSFDKTKVDSMILDDEIEYTIEDKGINVKTDTDNIKI
HHHCCCCEEEECCCCCCCCEEEECCCCCHHHHHEEECCCCEEEEECCCEEEEECCCEEEE
EFNPARFRPAEVPILFSNTAKIQKLGAKTEHSVSDIIKDQLNYFIKEENR
EECCCCCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKWQDKNVLITGISGFVGAYLAERLIKEGSNVYGLVRRRTDGSIPTNIKNHGIENDFTQI
CCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCCCCHHHH
TGDLTDISSVANAIDKSEPDYIFHLAAQSFVPQSFDNPTDTEQNNSQGTNNLLEAMRIKD
HCCHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
CDARMVFAGSSEEYGLVISSKDQYNAALKQYGSIFPDIKEGITHELPISETNPLRPMSPY
CCCEEEEECCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCH
AVSKVYGDFLTRNYYHSYGLDTVVSRAFNHEGAGRGKMFVTSVVTNQVIQLKLGLTDKIT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEHHHHHCCEEEEEECCCCEEE
IGNVNAFRDWTHIEDIINGYLLLAKKGNSGDVYNQGSMRTNSILSYILLSIEQIGQKIES
ECCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ISTLNGDKVITNPTELNSDKYYGVSFDKTKVDSMILDDEIEYTIEDKGINVKTDTDNIKI
HHHCCCCEEEECCCCCCCCEEEECCCCCHHHHHEEECCCCEEEEECCCEEEEECCCEEEE
EFNPARFRPAEVPILFSNTAKIQKLGAKTEHSVSDIIKDQLNYFIKEENR
EECCCCCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]