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The map label for this gene is birA [H]

Identifier: 84489714

GI number: 84489714

Start: 1048032

End: 1048607

Strand: Direct

Name: birA [H]

Synonym: Msp_0915

Alternate gene names: 84489714

Gene position: 1048032-1048607 (Clockwise)

Preceding gene: 84489678

Following gene: 84489717

Centisome position: 59.3

GC content: 30.03

Gene sequence:

>576_bases
ATGACCATGATACTAAGACCTGAAGTATCACTAAAAGAAGCTTCAAAACTCACAATAGTAACAGGTGTTGCAATAGCAAA
AACCTTACATGATGTATTTGATATTGATGTAGGTATTAAATGGCCTAATGACTTACTAATTGGAAATAAAAAGATTTGTG
GAATATTAACAGAAGCTGTAACAGATTATGATGAAATCAAAGCAGTACTTGTAGGTATAGGAGTAGATGTTAACTTTGAT
GAAGATAAACTTCCAGATAAACTAAAAGACGTTGCAACATCTGTTAAAGCAGAACTTGATACGGAATATAATAGGGCAGA
AATAATAAAAGAATTCTTTAAAGTATTTGAAGAATTATATGAACAATATAAAAAACATGACTTCAAATATATTGTATCTG
AGTGGAGAAGATTATCTACAACAACAGGTAATCGTGTGAAAGTATACACTGGACGAAAAGTAATTTATGCTGATGCAGTG
GGTATTACAAATGAGGGTGCATTGATTATTGAACATGAAGATGGAAAATTAGAAAAAGTAATTTCTGGAGAAATAGAAAT
AATAGATGATGAATAA

Upstream 100 bases:

>100_bases
ACAAGATGCAAAAGAAGGAACTATAATCATAGCAGAAAGTCAAACAGCAGGAAGAACAAGAAAACATGATGGATGGGTAT
CACCAGAAGGTGGTATTTAC

Downstream 100 bases:

>100_bases
ATCATCATCCATATCCCCCACTATTTTTGTTAATTAATTATATAATACAGATTCATTTTTGTTAATTAATTATATAATAC
AGATTCAAAGTCATTATTTT

Product: hypothetical protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MTMILRPEVSLKEASKLTIVTGVAIAKTLHDVFDIDVGIKWPNDLLIGNKKICGILTEAVTDYDEIKAVLVGIGVDVNFD
EDKLPDKLKDVATSVKAELDTEYNRAEIIKEFFKVFEELYEQYKKHDFKYIVSEWRRLSTTTGNRVKVYTGRKVIYADAV
GITNEGALIIEHEDGKLEKVISGEIEIIDDE

Sequences:

>Translated_191_residues
MTMILRPEVSLKEASKLTIVTGVAIAKTLHDVFDIDVGIKWPNDLLIGNKKICGILTEAVTDYDEIKAVLVGIGVDVNFD
EDKLPDKLKDVATSVKAELDTEYNRAEIIKEFFKVFEELYEQYKKHDFKYIVSEWRRLSTTTGNRVKVYTGRKVIYADAV
GITNEGALIIEHEDGKLEKVISGEIEIIDDE
>Mature_190_residues
TMILRPEVSLKEASKLTIVTGVAIAKTLHDVFDIDVGIKWPNDLLIGNKKICGILTEAVTDYDEIKAVLVGIGVDVNFDE
DKLPDKLKDVATSVKAELDTEYNRAEIIKEFFKVFEELYEQYKKHDFKYIVSEWRRLSTTTGNRVKVYTGRKVIYADAVG
ITNEGALIIEHEDGKLEKVISGEIEIIDDE

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=193, Percent_Identity=27.4611398963731, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 21597; Mature: 21466

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMILRPEVSLKEASKLTIVTGVAIAKTLHDVFDIDVGIKWPNDLLIGNKKICGILTEAV
CEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
TDYDEIKAVLVGIGVDVNFDEDKLPDKLKDVATSVKAELDTEYNRAEIIKEFFKVFEELY
CCHHHHHEEEEEECCCCCCCHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EQYKKHDFKYIVSEWRRLSTTTGNRVKVYTGRKVIYADAVGITNEGALIIEHEDGKLEKV
HHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCCCCCEEEEEECCCCEEHH
ISGEIEIIDDE
HCCCEEEEECC
>Mature Secondary Structure 
TMILRPEVSLKEASKLTIVTGVAIAKTLHDVFDIDVGIKWPNDLLIGNKKICGILTEAV
EEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
TDYDEIKAVLVGIGVDVNFDEDKLPDKLKDVATSVKAELDTEYNRAEIIKEFFKVFEELY
CCHHHHHEEEEEECCCCCCCHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EQYKKHDFKYIVSEWRRLSTTTGNRVKVYTGRKVIYADAVGITNEGALIIEHEDGKLEKV
HHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCCCCCEEEEEECCCCEEHH
ISGEIEIIDDE
HCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]