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The map label for this gene is hphIBM [H]

Identifier: 84489557

GI number: 84489557

Start: 867018

End: 867419

Strand: Direct

Name: hphIBM [H]

Synonym: Msp_0753

Alternate gene names: 84489557

Gene position: 867018-867419 (Clockwise)

Preceding gene: 84489556

Following gene: 84489558

Centisome position: 49.06

GC content: 21.89

Gene sequence:

>402_bases
ATGCAAAGCACGTTATTTAATTATAATAATCAAGATTTCAAATCTCAAAATAATTTTGATTCTTTCAAATTTCCTTCTAC
AAGATATCAAGGTAGTAAATTAAAACTCGTTGATTGGATAATAAATGAAACTAAAAATTATTCATATGAAACAGTTTTAG
ATGCTTTCGGAGGTACAGGTTCTGTTTCTTATTCTTATAAAAAAATAGGTAAAGAAGTGACATATAATGATATATTAAAA
TTTAATTACCAGTTTGGTAAAGCTTTAATTGAAAATAATGATATGAAATTAAGTAATGAATCAGTTAATTTTATTTTAAA
TCCACATGATGATATTGAATATAAAACAATAATACAAGACAATTTTAAAGATACATATTTTACTGATGATGAAAATAAAT
GA

Upstream 100 bases:

>100_bases
GTAAATTTAAAAAAAATAGTTAGAATATAAATTATAATTAACTTTTAATTTCTTTGATAAAAAATATCATAGTTAAATTT
TTTAAGGAGTATAGATGGAT

Downstream 100 bases:

>100_bases
TTAGATATGGTTATAACAAATATGAATTACCTGGATAATGAATTTGAATATGCAATTGCATTTTTTGCACTTTCCCAATC
TTGTATTATAAAAAGACCCT

Product: hypothetical protein

Products: NA

Alternate protein names: M.HphIB; Adenine-specific methyltransferase HphIB; M.HphI(A) [H]

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MQSTLFNYNNQDFKSQNNFDSFKFPSTRYQGSKLKLVDWIINETKNYSYETVLDAFGGTGSVSYSYKKIGKEVTYNDILK
FNYQFGKALIENNDMKLSNESVNFILNPHDDIEYKTIIQDNFKDTYFTDDENK

Sequences:

>Translated_133_residues
MQSTLFNYNNQDFKSQNNFDSFKFPSTRYQGSKLKLVDWIINETKNYSYETVLDAFGGTGSVSYSYKKIGKEVTYNDILK
FNYQFGKALIENNDMKLSNESVNFILNPHDDIEYKTIIQDNFKDTYFTDDENK
>Mature_133_residues
MQSTLFNYNNQDFKSQNNFDSFKFPSTRYQGSKLKLVDWIINETKNYSYETVLDAFGGTGSVSYSYKKIGKEVTYNDILK
FNYQFGKALIENNDMKLSNESVNFILNPHDDIEYKTIIQDNFKDTYFTDDENK

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 15650; Mature: 15650

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTLFNYNNQDFKSQNNFDSFKFPSTRYQGSKLKLVDWIINETKNYSYETVLDAFGGTG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHEEHHCCCCCCHHHHHHHCCCCC
SVSYSYKKIGKEVTYNDILKFNYQFGKALIENNDMKLSNESVNFILNPHDDIEYKTIIQD
CCEEHHHHHCCCCCCCHHEEECHHHHHHEECCCCEEECCCCEEEEECCCCCCCEEEEEEC
NFKDTYFTDDENK
CCCCCEECCCCCC
>Mature Secondary Structure
MQSTLFNYNNQDFKSQNNFDSFKFPSTRYQGSKLKLVDWIINETKNYSYETVLDAFGGTG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHEEHHCCCCCCHHHHHHHCCCCC
SVSYSYKKIGKEVTYNDILKFNYQFGKALIENNDMKLSNESVNFILNPHDDIEYKTIIQD
CCEEHHHHHCCCCCCCHHEEECHHHHHHEECCCCEEECCCCEEEEECCCCCCCEEEEEEC
NFKDTYFTDDENK
CCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]