Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 84489492

GI number: 84489492

Start: 796398

End: 797963

Strand: Direct

Name: thiE [H]

Synonym: Msp_0683

Alternate gene names: 84489492

Gene position: 796398-797963 (Clockwise)

Preceding gene: 84489491

Following gene: 84489499

Centisome position: 45.06

GC content: 29.57

Gene sequence:

>1566_bases
ATGGATATTGATTATAGTGTCTATTTAGTAACTGATCAATTTGATTTTACTGAATCAGAATTTTTAAATATTATAGAGGA
AGCTCTAAAAGGAAACACTACTATTGTTCAAATAAGAGAAAAAAATAGTAGTACAAGAGATTTTTTAAATCTTGCACAGA
AAGTTAAGAAATTAACTGATAAATATAATGTTCCATTAATTATTAATGATAGAATAGACATAGCTCTTGCAGTAGATTCT
GCAGGAGTTCATCTAGGTCAAGATGATATGCCTTGTAAAATAGCAAGAAAAATTATAGGACCAGATAAAATTATTGGAAT
CAGTGCTGAAAACTATGGGGATGCACTCCAAGCACAAGAAGATGGAGCAGACTATTTAGGTATAGGTGCTATAGAAAAAA
CTCCAACAAAAACAGATTGTAGTGTTATTTCAGAAGAGGATCTAAATAAAATTAAAAAAACAATAAAAATACCCCACGTA
GCAATAGGTGGTGTAAAAGAACATAATACTAGAGAAATAATAGATAAATATGGCTTTGATGGTGTAGCAATAGTTTCAGC
AATTATGAAAAGTGACCTTCCAAAAGAAGCTGCAACTAACTTTTATAATCTAGTAAATAGTAAGTTAACCTTAGATGATG
TTTATGCTGCTATATATGGTGTTATTGTAGGGGATGCTCTTGGTGTTCCATATGAATTTAAACCTAGATCTACACTACAA
AAAACACCTGTAACTGGAATGAGAGGTCATGGTACTTATGATAAACCAGAGGGCACTTGGTCTGATGATAGTTCTTTAAC
ATTAGCATTAGCTGATAGTTTAGTATATGGTATAGATTATGATGATATAATGTCTAGATTTAGTAAATGGTTATATCAAA
ACATGTATACTCCAGAGAATGAAGTTTTTGATGTTGGACAAACTACAGAAGAAGCAATCTATAATTATACAATGGGTGTA
AATCCATTAGATTGTGGTGGAGTTGAGGAATATGAAAATGGTAATGGTTCTTTAATGAGAATAATGCCATTATTATTATA
TATAAATAGAAAATCAATCGATGAAAAAAGTGCTATTGAATTAATAAATAATGTTTCATCATTAACACATGCACATAAAA
CTAGTAAAGCTTGTTGTAATATATATAATTTCCTAGTACAAGCTATTATTGAAAATAGGGAAGCTAATAATCTTAAAACT
TTAATTAAAAAAGCAATTGAAAGGGCTAAAAAACAGTATCCACTAAAAGAATATCCAGAATTTAAATCTTTATACGATGA
TCTATTCTTTGATTCAGGAGAAAATTTAGATAGTACTGGATATGTTGTAAATGCATTACAAATTGCATTCTATGCATGTT
ATCATAGTAGTGATTATAGTGATGCTGTGTTGTGTGCTGTGAATTTTGGAGAAGACACTGACACAAATGCTGCTATAACT
GGTGGGATTGCTGCTTTATACTATGGTTATAAATCTATTCCACCTAAATGGATCGATGTTATTATAAATAAGGAATTAAT
TAATGAAGTTTTAGATGCCTTTAGTGAAGGTCTTAAACATTATTAA

Upstream 100 bases:

>100_bases
ACAGGTTCATTTGGAATATATCTTATAGATGAATTATCAAAATTAAATAAAAGCACAATACTTTCTCAATCAAAATTAAA
TAAAATGTAGGTAATTGAAA

Downstream 100 bases:

>100_bases
TTTTCTCCTATTTTTTTTAGTATTTAATGAGTTTGGACCATTGTATATTTTTATCTAAAATTCTTTAAAAATTAGTTCTA
AACCAATAGCAAATCCCATA

Product: hypothetical protein

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MDIDYSVYLVTDQFDFTESEFLNIIEEALKGNTTIVQIREKNSSTRDFLNLAQKVKKLTDKYNVPLIINDRIDIALAVDS
AGVHLGQDDMPCKIARKIIGPDKIIGISAENYGDALQAQEDGADYLGIGAIEKTPTKTDCSVISEEDLNKIKKTIKIPHV
AIGGVKEHNTREIIDKYGFDGVAIVSAIMKSDLPKEAATNFYNLVNSKLTLDDVYAAIYGVIVGDALGVPYEFKPRSTLQ
KTPVTGMRGHGTYDKPEGTWSDDSSLTLALADSLVYGIDYDDIMSRFSKWLYQNMYTPENEVFDVGQTTEEAIYNYTMGV
NPLDCGGVEEYENGNGSLMRIMPLLLYINRKSIDEKSAIELINNVSSLTHAHKTSKACCNIYNFLVQAIIENREANNLKT
LIKKAIERAKKQYPLKEYPEFKSLYDDLFFDSGENLDSTGYVVNALQIAFYACYHSSDYSDAVLCAVNFGEDTDTNAAIT
GGIAALYYGYKSIPPKWIDVIINKELINEVLDAFSEGLKHY

Sequences:

>Translated_521_residues
MDIDYSVYLVTDQFDFTESEFLNIIEEALKGNTTIVQIREKNSSTRDFLNLAQKVKKLTDKYNVPLIINDRIDIALAVDS
AGVHLGQDDMPCKIARKIIGPDKIIGISAENYGDALQAQEDGADYLGIGAIEKTPTKTDCSVISEEDLNKIKKTIKIPHV
AIGGVKEHNTREIIDKYGFDGVAIVSAIMKSDLPKEAATNFYNLVNSKLTLDDVYAAIYGVIVGDALGVPYEFKPRSTLQ
KTPVTGMRGHGTYDKPEGTWSDDSSLTLALADSLVYGIDYDDIMSRFSKWLYQNMYTPENEVFDVGQTTEEAIYNYTMGV
NPLDCGGVEEYENGNGSLMRIMPLLLYINRKSIDEKSAIELINNVSSLTHAHKTSKACCNIYNFLVQAIIENREANNLKT
LIKKAIERAKKQYPLKEYPEFKSLYDDLFFDSGENLDSTGYVVNALQIAFYACYHSSDYSDAVLCAVNFGEDTDTNAAIT
GGIAALYYGYKSIPPKWIDVIINKELINEVLDAFSEGLKHY
>Mature_521_residues
MDIDYSVYLVTDQFDFTESEFLNIIEEALKGNTTIVQIREKNSSTRDFLNLAQKVKKLTDKYNVPLIINDRIDIALAVDS
AGVHLGQDDMPCKIARKIIGPDKIIGISAENYGDALQAQEDGADYLGIGAIEKTPTKTDCSVISEEDLNKIKKTIKIPHV
AIGGVKEHNTREIIDKYGFDGVAIVSAIMKSDLPKEAATNFYNLVNSKLTLDDVYAAIYGVIVGDALGVPYEFKPRSTLQ
KTPVTGMRGHGTYDKPEGTWSDDSSLTLALADSLVYGIDYDDIMSRFSKWLYQNMYTPENEVFDVGQTTEEAIYNYTMGV
NPLDCGGVEEYENGNGSLMRIMPLLLYINRKSIDEKSAIELINNVSSLTHAHKTSKACCNIYNFLVQAIIENREANNLKT
LIKKAIERAKKQYPLKEYPEFKSLYDDLFFDSGENLDSTGYVVNALQIAFYACYHSSDYSDAVLCAVNFGEDTDTNAAIT
GGIAALYYGYKSIPPKWIDVIINKELINEVLDAFSEGLKHY

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG1397

COG function: function code O; ADP-ribosylglycohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=210, Percent_Identity=37.6190476190476, Blast_Score=133, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 58057; Mature: 58057

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIDYSVYLVTDQFDFTESEFLNIIEEALKGNTTIVQIREKNSSTRDFLNLAQKVKKLTD
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
KYNVPLIINDRIDIALAVDSAGVHLGQDDMPCKIARKIIGPDKIIGISAENYGDALQAQE
HCCCCEEECCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCHHHHHC
DGADYLGIGAIEKTPTKTDCSVISEEDLNKIKKTIKIPHVAIGGVKEHNTREIIDKYGFD
CCCCEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCC
GVAIVSAIMKSDLPKEAATNFYNLVNSKLTLDDVYAAIYGVIVGDALGVPYEFKPRSTLQ
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
KTPVTGMRGHGTYDKPEGTWSDDSSLTLALADSLVYGIDYDDIMSRFSKWLYQNMYTPEN
HCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
EVFDVGQTTEEAIYNYTMGVNPLDCGGVEEYENGNGSLMRIMPLLLYINRKSIDEKSAIE
CEEECCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
LINNVSSLTHAHKTSKACCNIYNFLVQAIIENREANNLKTLIKKAIERAKKQYPLKEYPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCH
FKSLYDDLFFDSGENLDSTGYVVNALQIAFYACYHSSDYSDAVLCAVNFGEDTDTNAAIT
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHH
GGIAALYYGYKSIPPKWIDVIINKELINEVLDAFSEGLKHY
HHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIDYSVYLVTDQFDFTESEFLNIIEEALKGNTTIVQIREKNSSTRDFLNLAQKVKKLTD
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
KYNVPLIINDRIDIALAVDSAGVHLGQDDMPCKIARKIIGPDKIIGISAENYGDALQAQE
HCCCCEEECCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCHHHHHC
DGADYLGIGAIEKTPTKTDCSVISEEDLNKIKKTIKIPHVAIGGVKEHNTREIIDKYGFD
CCCCEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCC
GVAIVSAIMKSDLPKEAATNFYNLVNSKLTLDDVYAAIYGVIVGDALGVPYEFKPRSTLQ
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
KTPVTGMRGHGTYDKPEGTWSDDSSLTLALADSLVYGIDYDDIMSRFSKWLYQNMYTPEN
HCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
EVFDVGQTTEEAIYNYTMGVNPLDCGGVEEYENGNGSLMRIMPLLLYINRKSIDEKSAIE
CEEECCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
LINNVSSLTHAHKTSKACCNIYNFLVQAIIENREANNLKTLIKKAIERAKKQYPLKEYPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCH
FKSLYDDLFFDSGENLDSTGYVVNALQIAFYACYHSSDYSDAVLCAVNFGEDTDTNAAIT
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHH
GGIAALYYGYKSIPPKWIDVIINKELINEVLDAFSEGLKHY
HHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA