Click here to switch to the map view.

The map label for this gene is thsB

Identifier: 84488944

GI number: 84488944

Start: 128037

End: 129653

Strand: Direct

Name: thsB

Synonym: Msp_0114

Alternate gene names: 84488944

Gene position: 128037-129653 (Clockwise)

Preceding gene: 84488943

Following gene: 84488945

Centisome position: 7.24

GC content: 36.43

Gene sequence:

>1617_bases
ATGGCACAACAACAACAACCTTTAATTATCTTACCTGAAGGAACAACAAGAAACTTAGGTAGAGATGCACAAAGAAATAA
TATTTTAGCTGGAAAAGTTTTAGCAGAAACAGTAAGAACAACACTTGGTCCTAAAGGTATGGACAAAATGTTAGTAGACG
GTCTTGGAGACATAGTTGTAACAAACGACGGTGTAACAATCCTTAAAGAAATGGATATTGAACACCCAGCAGCAAAAATG
TTAGTAGAAGTAGCAAAAACACAAGAAGACGAAGTTGGAGATGGAACAACAACAGCTGTAATAATTGCTGGTGAATTACT
CAAAAGATCAGAAGAATTATTAGACCAAGAAATCCACCCAACAATCATTGCATTAGGATACAGACAAGCAGCTCAAAAAG
CAATAGAATTATTAGATCAAATATCCATTGATGCTGATGATAAAGACACTCTTCTAAAAGTTGCAATGACAGCAATGACA
GGAAAAGGAACAGAAAAAGCAAGAGAACCATTAGCAGAACTTATTGTAGGGGCAGTAAGTCAAGTAGTTGAAGATGGTAA
AGTAGATTCAGAACAAATAAAAATTGAATCTAAAGATGGAGCAGCTATTGAAGATTCAGAATTAGTATCTGGAGTAATCA
TAGACAAAGAAAAAGTACATCCTGGTATGCCATCAGAAATAAATGGTGCAAAAATTGCATTAGTTAACAGTGCAATAGAA
GTAAAAGAAACAGAAGTAGATGCAGAAATAAGAATTACTGACCCAGCACAAATGCAAGCATTCATTGAACAAGAAGAAAA
CATGATTAAAGAAATGGTTGATGAATTAGTAGCAGCTGGAGCAAATGTATTATTCTGTCAAAAAGGTATCGATGACTTAG
CACAACACTACTTATCCAAAGCAGGTATATTAGCAACAAGAAGAGTTAAAAAATCTGACATGGAAAAATTAGCAAAAGCA
ACTGGTGCAAAAATCGTAACAAACATCGAAGATTTATCTGCAGACGACCTTGGAGATGCAGGATTAGTAGCTGAAGATAA
AGTATCTGGTGATGACATGATCTTTGTAAAAGAATGTAAAGATCCAAAAGCAGTAACTTTATTATTAAGAGGTTCCACTT
CCCACGTAGTAGATGAAATTGAAAGAGCAGTAGAAGATGCAATAGGAGTAGTAGCTTCCACAGTAGAAGACGGTAAAGTT
GTTGTTGGTGGAGGAGCTCCAGAAATAGCAATTGCAAAAGGATTAAAAGACTACGCAGAAACAATAAGTGGAAGAGAACA
ATTAGCAGTAACTGCATTTGCAGAAGCATTAGAAGTAGTTCCAAGAACACTTGCAGAAAATGCAGGTCTCGACAGTATTG
ACTCCTTAGTAGATTTAAGAGCAGCTCACGAAGATTCAATATACATGGGATTAAATGTATTTGAAGGTGGAGTAACTGAC
ATGAAAGAAGCAGGAGTTATTGAACCTCAAAGAGTTAAAAAACAAGCTATACAATCTGCAGCAGAAGCAGCTGAAATGAT
CTTAAGAATCGATGATGTAATAGCATCCTCAAGTCAAGGTGCAGCAGCTGGAGCACCAGCACCTGATATGGGTATGGGCG
GAATGCCTCCAATGTAA

Upstream 100 bases:

>100_bases
TATATTTTAATGAAAACTAATTTAAACTAAAAAAATTGAAAAAATATGGATTACATCCATAATTTAATATTAACAATATA
ATTTAATAGAGGTAATATAT

Downstream 100 bases:

>100_bases
ATATAGATTATAATCTATATTAGTTAAATTACCTATGAGTTAGAGAGATATATTAGTCTCTATAAGGATTGGTTCATGAT
AGACTCATGAACTTACTTTT

Product: ThsB

Products: NA

Alternate protein names: Chaperonin subunit beta; Thermosome subunit 2 [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAQQQQPLIILPEGTTRNLGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVVTNDGVTILKEMDIEHPAAKM
LVEVAKTQEDEVGDGTTTAVIIAGELLKRSEELLDQEIHPTIIALGYRQAAQKAIELLDQISIDADDKDTLLKVAMTAMT
GKGTEKAREPLAELIVGAVSQVVEDGKVDSEQIKIESKDGAAIEDSELVSGVIIDKEKVHPGMPSEINGAKIALVNSAIE
VKETEVDAEIRITDPAQMQAFIEQEENMIKEMVDELVAAGANVLFCQKGIDDLAQHYLSKAGILATRRVKKSDMEKLAKA
TGAKIVTNIEDLSADDLGDAGLVAEDKVSGDDMIFVKECKDPKAVTLLLRGSTSHVVDEIERAVEDAIGVVASTVEDGKV
VVGGGAPEIAIAKGLKDYAETISGREQLAVTAFAEALEVVPRTLAENAGLDSIDSLVDLRAAHEDSIYMGLNVFEGGVTD
MKEAGVIEPQRVKKQAIQSAAEAAEMILRIDDVIASSSQGAAAGAPAPDMGMGGMPPM

Sequences:

>Translated_538_residues
MAQQQQPLIILPEGTTRNLGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVVTNDGVTILKEMDIEHPAAKM
LVEVAKTQEDEVGDGTTTAVIIAGELLKRSEELLDQEIHPTIIALGYRQAAQKAIELLDQISIDADDKDTLLKVAMTAMT
GKGTEKAREPLAELIVGAVSQVVEDGKVDSEQIKIESKDGAAIEDSELVSGVIIDKEKVHPGMPSEINGAKIALVNSAIE
VKETEVDAEIRITDPAQMQAFIEQEENMIKEMVDELVAAGANVLFCQKGIDDLAQHYLSKAGILATRRVKKSDMEKLAKA
TGAKIVTNIEDLSADDLGDAGLVAEDKVSGDDMIFVKECKDPKAVTLLLRGSTSHVVDEIERAVEDAIGVVASTVEDGKV
VVGGGAPEIAIAKGLKDYAETISGREQLAVTAFAEALEVVPRTLAENAGLDSIDSLVDLRAAHEDSIYMGLNVFEGGVTD
MKEAGVIEPQRVKKQAIQSAAEAAEMILRIDDVIASSSQGAAAGAPAPDMGMGGMPPM
>Mature_537_residues
AQQQQPLIILPEGTTRNLGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVVTNDGVTILKEMDIEHPAAKML
VEVAKTQEDEVGDGTTTAVIIAGELLKRSEELLDQEIHPTIIALGYRQAAQKAIELLDQISIDADDKDTLLKVAMTAMTG
KGTEKAREPLAELIVGAVSQVVEDGKVDSEQIKIESKDGAAIEDSELVSGVIIDKEKVHPGMPSEINGAKIALVNSAIEV
KETEVDAEIRITDPAQMQAFIEQEENMIKEMVDELVAAGANVLFCQKGIDDLAQHYLSKAGILATRRVKKSDMEKLAKAT
GAKIVTNIEDLSADDLGDAGLVAEDKVSGDDMIFVKECKDPKAVTLLLRGSTSHVVDEIERAVEDAIGVVASTVEDGKVV
VGGGAPEIAIAKGLKDYAETISGREQLAVTAFAEALEVVPRTLAENAGLDSIDSLVDLRAAHEDSIYMGLNVFEGGVTDM
KEAGVIEPQRVKKQAIQSAAEAAEMILRIDDVIASSSQGAAAGAPAPDMGMGGMPPM

Specific function: Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TCP-1 chaperonin family [H]

Homologues:

Organism=Homo sapiens, GI63162572, Length=537, Percent_Identity=39.8510242085661, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI24307939, Length=520, Percent_Identity=40.1923076923077, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI38455427, Length=502, Percent_Identity=40.4382470119522, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI57863257, Length=521, Percent_Identity=37.6199616122841, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI5453607, Length=518, Percent_Identity=37.2586872586873, Blast_Score=348, Evalue=9e-96,
Organism=Homo sapiens, GI58761484, Length=537, Percent_Identity=35.7541899441341, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI261399877, Length=478, Percent_Identity=35.9832635983264, Blast_Score=310, Evalue=3e-84,
Organism=Homo sapiens, GI58331173, Length=500, Percent_Identity=36.2, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI4502643, Length=500, Percent_Identity=35.2, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI5453603, Length=522, Percent_Identity=33.7164750957854, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI302058290, Length=493, Percent_Identity=36.3083164300203, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI261399875, Length=433, Percent_Identity=33.7182448036951, Blast_Score=255, Evalue=8e-68,
Organism=Homo sapiens, GI48762932, Length=535, Percent_Identity=34.392523364486, Blast_Score=245, Evalue=8e-65,
Organism=Homo sapiens, GI302058292, Length=500, Percent_Identity=32.4, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI58331171, Length=500, Percent_Identity=31.2, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI57863259, Length=337, Percent_Identity=36.2017804154303, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI58331185, Length=314, Percent_Identity=34.7133757961783, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI7657253, Length=492, Percent_Identity=27.4390243902439, Blast_Score=182, Evalue=1e-45,
Organism=Homo sapiens, GI41399285, Length=558, Percent_Identity=24.3727598566308, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI31542947, Length=558, Percent_Identity=24.3727598566308, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI25914754, Length=465, Percent_Identity=21.7204301075269, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI9055272, Length=465, Percent_Identity=21.7204301075269, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1790586, Length=537, Percent_Identity=24.3947858472998, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144674, Length=518, Percent_Identity=39.96138996139, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17532601, Length=520, Percent_Identity=38.6538461538462, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17532603, Length=505, Percent_Identity=40.5940594059406, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI25148561, Length=537, Percent_Identity=35.3817504655494, Blast_Score=339, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17564182, Length=531, Percent_Identity=32.7683615819209, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25147750, Length=526, Percent_Identity=35.361216730038, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25144678, Length=507, Percent_Identity=35.8974358974359, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI32566944, Length=440, Percent_Identity=31.5909090909091, Blast_Score=260, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI25144680, Length=387, Percent_Identity=35.4005167958656, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71981457, Length=325, Percent_Identity=38.7692307692308, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI71998178, Length=561, Percent_Identity=28.3422459893048, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17555558, Length=569, Percent_Identity=24.9560632688928, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322446, Length=527, Percent_Identity=36.0531309297913, Blast_Score=352, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6322524, Length=531, Percent_Identity=37.6647834274953, Blast_Score=344, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6320418, Length=524, Percent_Identity=36.8320610687023, Blast_Score=327, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6322350, Length=549, Percent_Identity=34.2440801457195, Blast_Score=317, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6320058, Length=510, Percent_Identity=36.078431372549, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6322049, Length=521, Percent_Identity=31.6698656429942, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6320393, Length=523, Percent_Identity=30.5927342256214, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6322452, Length=535, Percent_Identity=31.7757009345794, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6323288, Length=216, Percent_Identity=26.3888888888889, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17647245, Length=527, Percent_Identity=40.607210626186, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24647512, Length=531, Percent_Identity=38.6064030131827, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24647510, Length=531, Percent_Identity=38.6064030131827, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24652903, Length=507, Percent_Identity=40.6311637080868, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24649027, Length=537, Percent_Identity=37.6163873370577, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24649029, Length=537, Percent_Identity=37.6163873370577, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24583944, Length=511, Percent_Identity=38.3561643835616, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24645179, Length=526, Percent_Identity=35.7414448669202, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI18858175, Length=505, Percent_Identity=35.2475247524752, Blast_Score=291, Evalue=1e-78,
Organism=Drosophila melanogaster, GI28571140, Length=505, Percent_Identity=35.2475247524752, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI18859933, Length=504, Percent_Identity=35.7142857142857, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI19921848, Length=509, Percent_Identity=31.8271119842829, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550936, Length=551, Percent_Identity=25.4083484573503, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI45550132, Length=551, Percent_Identity=25.4083484573503, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI45550935, Length=551, Percent_Identity=25.4083484573503, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17864606, Length=562, Percent_Identity=23.6654804270463, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017998
- InterPro:   IPR002194
- InterPro:   IPR002423
- InterPro:   IPR012714 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57013; Mature: 56882

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS00750 TCP1_1 ; PS00751 TCP1_2 ; PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQQQQPLIILPEGTTRNLGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVV
CCCCCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEE
TNDGVTILKEMDIEHPAAKMLVEVAKTQEDEVGDGTTTAVIIAGELLKRSEELLDQEIHP
ECCCEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCC
TIIALGYRQAAQKAIELLDQISIDADDKDTLLKVAMTAMTGKGTEKAREPLAELIVGAVS
CEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QVVEDGKVDSEQIKIESKDGAAIEDSELVSGVIIDKEKVHPGMPSEINGAKIALVNSAIE
HHHHCCCCCCHHEEEECCCCCCCCHHHHHHEEEEEHHHCCCCCCCCCCCCEEEEEECHHH
VKETEVDAEIRITDPAQMQAFIEQEENMIKEMVDELVAAGANVLFCQKGIDDLAQHYLSK
EECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
AGILATRRVKKSDMEKLAKATGAKIVTNIEDLSADDLGDAGLVAEDKVSGDDMIFVKECK
HCHHHHHHHHHHHHHHHHHHCCCCEEECHHHCCCCCCCCCCCEECCCCCCCCEEEEECCC
DPKAVTLLLRGSTSHVVDEIERAVEDAIGVVASTVEDGKVVVGGGAPEIAIAKGLKDYAE
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHH
TISGREQLAVTAFAEALEVVPRTLAENAGLDSIDSLVDLRAAHEDSIYMGLNVFEGGVTD
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCC
MKEAGVIEPQRVKKQAIQSAAEAAEMILRIDDVIASSSQGAAAGAPAPDMGMGGMPPM
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQQQQPLIILPEGTTRNLGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVV
CCCCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEE
TNDGVTILKEMDIEHPAAKMLVEVAKTQEDEVGDGTTTAVIIAGELLKRSEELLDQEIHP
ECCCEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCC
TIIALGYRQAAQKAIELLDQISIDADDKDTLLKVAMTAMTGKGTEKAREPLAELIVGAVS
CEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QVVEDGKVDSEQIKIESKDGAAIEDSELVSGVIIDKEKVHPGMPSEINGAKIALVNSAIE
HHHHCCCCCCHHEEEECCCCCCCCHHHHHHEEEEEHHHCCCCCCCCCCCCEEEEEECHHH
VKETEVDAEIRITDPAQMQAFIEQEENMIKEMVDELVAAGANVLFCQKGIDDLAQHYLSK
EECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
AGILATRRVKKSDMEKLAKATGAKIVTNIEDLSADDLGDAGLVAEDKVSGDDMIFVKECK
HCHHHHHHHHHHHHHHHHHHCCCCEEECHHHCCCCCCCCCCCEECCCCCCCCEEEEECCC
DPKAVTLLLRGSTSHVVDEIERAVEDAIGVVASTVEDGKVVVGGGAPEIAIAKGLKDYAE
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHH
TISGREQLAVTAFAEALEVVPRTLAENAGLDSIDSLVDLRAAHEDSIYMGLNVFEGGVTD
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCC
MKEAGVIEPQRVKKQAIQSAAEAAEMILRIDDVIASSSQGAAAGAPAPDMGMGGMPPM
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]