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The map label for this gene is priA

Identifier: 84488915

GI number: 84488915

Start: 100419

End: 101393

Strand: Direct

Name: priA

Synonym: Msp_0085

Alternate gene names: 84488915

Gene position: 100419-101393 (Clockwise)

Preceding gene: 84488914

Following gene: 84488926

Centisome position: 5.68

GC content: 30.15

Gene sequence:

>975_bases
ATGAGTGTTGAATTAAAACCTGCATCTAAATATGAACGTAAAATCTTTTATAAAGAAGAATGGAATGTGAAAGATGTTCC
AGATTTCATAAGAAATTCTCTTACAAGTCGTGAATTTGGTTTTGATCATTATGGTAATGGACCAAATGATCGTTATAAGG
TATTTGTTGATGATTTACGATTAAAACGTTTTATAAAAGTAAAACAACCATTTGCTGCATATTGTTCTGTGGCATTCTAT
GATAAGCCAAATCAGAGAAAGGGTTGGCAGAAATCAGAATTAGTATTTGATGTAGATGCAAAGGATATTCCAATAAGAAC
ATGTGACTGTGCTGAAGGAGAAGTATGTGAAAAATGCTTAAATCAGGCAAAGGAGATAGTTTTAATGATAAGGGATGTTC
TTAGTGGTGATTTTGGACTAACAAATATAAATCTCATATATTCAGGTAGGGGATACCATGTACGTGTTCTTGATGAAGAT
GTGATGGATGCATCAAGTGAGTTACGTGGGGATATAATACAGTATGTTACTGGTTCAAAACTACCTGATTTATCAAATGA
GTATGGATTTAATGATTTACAACCATTTATTATACCATTTGGTTATCCAAAGAACTTTACAAGATGGTCAAAATATACAA
TTTTACATCTAAGACGATCTTCTAAATTAGATGATGTTAATAATCAATTATTAAGGGATGTTTTAAAACATAGAAATCTA
TTACAAGAAGATTTATGGGGTATGTTTAGGAGTAATATTGGACCTATACGTTATAAAAAGGTGATGAATGCAATTTCTAG
AATTAATATGCAAATTACTGATACTAAGGTATCAATTGACTTAAAACGTATATTAAGACTTCCATCCACATTACATTCAA
AGGTAAGTATGAAATCTACTCTAATAAAGAATATAGAAACATTTGATCCCTTTGATGATGCTGTTCCAAAATTTGTTTAT
GAACGTAATGATTAA

Upstream 100 bases:

>100_bases
GTGTTGATTGTAAAAAAATAGGAAATCCCTTGATATATTATAATCGTAAGAAAAGATTACTTGAAAAAAATAATTCAAAA
GCAACAGCAGGTGAAGAAAA

Downstream 100 bases:

>100_bases
TGTTCTAATCTATAATTTGGATGTAAATATAGGAATATGTTGTTTAAGTTTTCATCATTTGTATTGTTAATAAATACTTC
CATTTTTTCAAAAGAATAGT

Product: DNA primase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSVELKPASKYERKIFYKEEWNVKDVPDFIRNSLTSREFGFDHYGNGPNDRYKVFVDDLRLKRFIKVKQPFAAYCSVAFY
DKPNQRKGWQKSELVFDVDAKDIPIRTCDCAEGEVCEKCLNQAKEIVLMIRDVLSGDFGLTNINLIYSGRGYHVRVLDED
VMDASSELRGDIIQYVTGSKLPDLSNEYGFNDLQPFIIPFGYPKNFTRWSKYTILHLRRSSKLDDVNNQLLRDVLKHRNL
LQEDLWGMFRSNIGPIRYKKVMNAISRINMQITDTKVSIDLKRILRLPSTLHSKVSMKSTLIKNIETFDPFDDAVPKFVY
ERND

Sequences:

>Translated_324_residues
MSVELKPASKYERKIFYKEEWNVKDVPDFIRNSLTSREFGFDHYGNGPNDRYKVFVDDLRLKRFIKVKQPFAAYCSVAFY
DKPNQRKGWQKSELVFDVDAKDIPIRTCDCAEGEVCEKCLNQAKEIVLMIRDVLSGDFGLTNINLIYSGRGYHVRVLDED
VMDASSELRGDIIQYVTGSKLPDLSNEYGFNDLQPFIIPFGYPKNFTRWSKYTILHLRRSSKLDDVNNQLLRDVLKHRNL
LQEDLWGMFRSNIGPIRYKKVMNAISRINMQITDTKVSIDLKRILRLPSTLHSKVSMKSTLIKNIETFDPFDDAVPKFVY
ERND
>Mature_323_residues
SVELKPASKYERKIFYKEEWNVKDVPDFIRNSLTSREFGFDHYGNGPNDRYKVFVDDLRLKRFIKVKQPFAAYCSVAFYD
KPNQRKGWQKSELVFDVDAKDIPIRTCDCAEGEVCEKCLNQAKEIVLMIRDVLSGDFGLTNINLIYSGRGYHVRVLDEDV
MDASSELRGDIIQYVTGSKLPDLSNEYGFNDLQPFIIPFGYPKNFTRWSKYTILHLRRSSKLDDVNNQLLRDVLKHRNLL
QEDLWGMFRSNIGPIRYKKVMNAISRINMQITDTKVSIDLKRILRLPSTLHSKVSMKSTLIKNIETFDPFDDAVPKFVYE
RND

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG1467

COG function: function code L; Eukaryotic-type DNA primase, catalytic (small) subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase small subunit family

Homologues:

Organism=Homo sapiens, GI4506051, Length=342, Percent_Identity=24.5614035087719, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIS_METST (Q2NI23)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447147.1
- ProteinModelPortal:   Q2NI23
- SMR:   Q2NI23
- STRING:   Q2NI23
- GeneID:   3855614
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0085
- NMPDR:   fig|339860.6.peg.84
- eggNOG:   arNOG05167
- HOGENOM:   HBG551858
- OMA:   REFGFDH
- ProtClustDB:   PRK00419
- BioCyc:   MSTA339860:MSP_0085-MONOMER
- HAMAP:   MF_00700
- InterPro:   IPR002755
- InterPro:   IPR014052
- PANTHER:   PTHR10536
- TIGRFAMs:   TIGR00335

Pfam domain/function: PF01896 DNA_primase_S

EC number: 2.7.7.-

Molecular weight: Translated: 37841; Mature: 37710

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: ACT_SITE 97-97 ACT_SITE 99-99

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVELKPASKYERKIFYKEEWNVKDVPDFIRNSLTSREFGFDHYGNGPNDRYKVFVDDLR
CCCCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHH
LKRFIKVKQPFAAYCSVAFYDKPNQRKGWQKSELVFDVDAKDIPIRTCDCAEGEVCEKCL
HHHHHHHCCCHHHHHEEEEECCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCHHHHHHH
NQAKEIVLMIRDVLSGDFGLTNINLIYSGRGYHVRVLDEDVMDASSELRGDIIQYVTGSK
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCC
LPDLSNEYGFNDLQPFIIPFGYPKNFTRWSKYTILHLRRSSKLDDVNNQLLRDVLKHRNL
CCCCCCCCCCCCCCCEEEECCCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHH
LQEDLWGMFRSNIGPIRYKKVMNAISRINMQITDTKVSIDLKRILRLPSTLHSKVSMKST
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHCHHHHHHHHHHHHH
LIKNIETFDPFDDAVPKFVYERND
HHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVELKPASKYERKIFYKEEWNVKDVPDFIRNSLTSREFGFDHYGNGPNDRYKVFVDDLR
CCCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHH
LKRFIKVKQPFAAYCSVAFYDKPNQRKGWQKSELVFDVDAKDIPIRTCDCAEGEVCEKCL
HHHHHHHCCCHHHHHEEEEECCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCHHHHHHH
NQAKEIVLMIRDVLSGDFGLTNINLIYSGRGYHVRVLDEDVMDASSELRGDIIQYVTGSK
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCC
LPDLSNEYGFNDLQPFIIPFGYPKNFTRWSKYTILHLRRSSKLDDVNNQLLRDVLKHRNL
CCCCCCCCCCCCCCCEEEECCCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHH
LQEDLWGMFRSNIGPIRYKKVMNAISRINMQITDTKVSIDLKRILRLPSTLHSKVSMKST
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHCHHHHHHHHHHHHH
LIKNIETFDPFDDAVPKFVYERND
HHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA