Click here to switch to the map view.

The map label for this gene is priB [H]

Identifier: 84488914

GI number: 84488914

Start: 99079

End: 100422

Strand: Direct

Name: priB [H]

Synonym: Msp_0084

Alternate gene names: 84488914

Gene position: 99079-100422 (Clockwise)

Preceding gene: 84488909

Following gene: 84488915

Centisome position: 5.61

GC content: 29.09

Gene sequence:

>1344_bases
ATGATAGAAACTTCATTTATAAATCCATTTAGCTTTGAGGCTAAAGAAATTGTAAGAAAATTAGGACAAATTGAAAATTT
AGATAAGAAAAATGATTCATTAATAGATATTATAAAACATACACATTCACAGAAACTTTCATCAATACCTGAAACATTAG
AACAATTAGCTCTACATAAATTCCAATGGTACTTAGATAAAAAAACTGATAACTTTGATGATAAGGTTTATGAATATTTA
TTTAATCCTGAAATTTATGAATATGATGTGCTATCCTTTTATTTTTTATGTCAAGCTGTAGCAATAGGTTTTGGTGAGGA
TAGTCACGAAGCAAAACAAGTTGTAGAATTAGAAGCTAATCTTATAGATAATAGATTAGAAAGACTAAAAAGTGAACCTA
ATAATTTTCAGTATAGTTTTCTTAAAGAAGCATTAAATCAACTAATTGATACAAATAATGTCTACTGGAATGACTTAAAA
GAAGTTATAGACCTTGGAGTAATCGACTTAAATAAATTATTACTGTCAAATGGTAGGGTTATTATAGAATATGAAGACTT
TATAGAAGAATATGGACATTTAATTGAACATAGGAATCCACAAACAATGTATGAGGTAATATGTGGATTAAATCTAAAGA
AAAATCTATTAAAAAGTATAATAATGAAAAAAACTAGGGATTATATTGTGAAGGTTGCTGAAAATTCTAGGAGAATGGTT
GAACCTGATTCAATTATGAATGAATTATCAGGTAAAATAAGACAAATGGAACAAAAGGCAAGGGAGAGAAGATTTGGTTC
ATCAAATAGTGGTGCATATAATGATAATGGACCTGCACCATACATACAAGAGGCATTTCCACCATGTGTACGTAAGTGTA
TGGAGGGAATTAAAAGTGGTGGAAGAAACGATGCAATTGTGTTGTTTTTAACTCCATTTATATCATATGCTAGATTGGCA
CCAAATGTATTTTCAATGGAATATGATATGAAAGTATCTGATGTGGATCCATCACTTGAAATAACACATAAAGAAGTAAT
TCCAATGATTTATGAGGCTGCAAATGCTTGTAGACCACCATTATTCAAGGATCAGCCACAAGAAAAGATTAACATCAATT
CTAAACTTGGTTTTGGTATGAATTCAGAATTAAAACTATCAGAGGAGGGTGAAACTAGGTGGTATACACCTATGAGTTGT
GAAAAAATCAAATTACACATGCCAAATTTATGTATTCCTTGTGTTGATTGTAAAAAAATAGGAAATCCCTTGATATATTA
TAATCGTAAGAAAAGATTACTTGAAAAAAATAATTCAAAAGCAACAGCAGGTGAAGAAAAATGA

Upstream 100 bases:

>100_bases
GAATATCAATTATAATTAATATTTATATATGGTGTTCATGATTTATTTATTTTACTAGGTAAAAGATTATTACATTATAG
GTTATAGATATTATATTATC

Downstream 100 bases:

>100_bases
GTGTTGAATTAAAACCTGCATCTAAATATGAACGTAAAATCTTTTATAAAGAAGAATGGAATGTGAAAGATGTTCCAGAT
TTCATAAGAAATTCTCTTAC

Product: DNA primase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MIETSFINPFSFEAKEIVRKLGQIENLDKKNDSLIDIIKHTHSQKLSSIPETLEQLALHKFQWYLDKKTDNFDDKVYEYL
FNPEIYEYDVLSFYFLCQAVAIGFGEDSHEAKQVVELEANLIDNRLERLKSEPNNFQYSFLKEALNQLIDTNNVYWNDLK
EVIDLGVIDLNKLLLSNGRVIIEYEDFIEEYGHLIEHRNPQTMYEVICGLNLKKNLLKSIIMKKTRDYIVKVAENSRRMV
EPDSIMNELSGKIRQMEQKARERRFGSSNSGAYNDNGPAPYIQEAFPPCVRKCMEGIKSGGRNDAIVLFLTPFISYARLA
PNVFSMEYDMKVSDVDPSLEITHKEVIPMIYEAANACRPPLFKDQPQEKININSKLGFGMNSELKLSEEGETRWYTPMSC
EKIKLHMPNLCIPCVDCKKIGNPLIYYNRKKRLLEKNNSKATAGEEK

Sequences:

>Translated_447_residues
MIETSFINPFSFEAKEIVRKLGQIENLDKKNDSLIDIIKHTHSQKLSSIPETLEQLALHKFQWYLDKKTDNFDDKVYEYL
FNPEIYEYDVLSFYFLCQAVAIGFGEDSHEAKQVVELEANLIDNRLERLKSEPNNFQYSFLKEALNQLIDTNNVYWNDLK
EVIDLGVIDLNKLLLSNGRVIIEYEDFIEEYGHLIEHRNPQTMYEVICGLNLKKNLLKSIIMKKTRDYIVKVAENSRRMV
EPDSIMNELSGKIRQMEQKARERRFGSSNSGAYNDNGPAPYIQEAFPPCVRKCMEGIKSGGRNDAIVLFLTPFISYARLA
PNVFSMEYDMKVSDVDPSLEITHKEVIPMIYEAANACRPPLFKDQPQEKININSKLGFGMNSELKLSEEGETRWYTPMSC
EKIKLHMPNLCIPCVDCKKIGNPLIYYNRKKRLLEKNNSKATAGEEK
>Mature_447_residues
MIETSFINPFSFEAKEIVRKLGQIENLDKKNDSLIDIIKHTHSQKLSSIPETLEQLALHKFQWYLDKKTDNFDDKVYEYL
FNPEIYEYDVLSFYFLCQAVAIGFGEDSHEAKQVVELEANLIDNRLERLKSEPNNFQYSFLKEALNQLIDTNNVYWNDLK
EVIDLGVIDLNKLLLSNGRVIIEYEDFIEEYGHLIEHRNPQTMYEVICGLNLKKNLLKSIIMKKTRDYIVKVAENSRRMV
EPDSIMNELSGKIRQMEQKARERRFGSSNSGAYNDNGPAPYIQEAFPPCVRKCMEGIKSGGRNDAIVLFLTPFISYARLA
PNVFSMEYDMKVSDVDPSLEITHKEVIPMIYEAANACRPPLFKDQPQEKININSKLGFGMNSELKLSEEGETRWYTPMSC
EKIKLHMPNLCIPCVDCKKIGNPLIYYNRKKRLLEKNNSKATAGEEK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007238 [H]

Pfam domain/function: PF04104 DNA_primase_lrg [H]

EC number: NA

Molecular weight: Translated: 51781; Mature: 51781

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEEECHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure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CCCEEEECHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]