Definition Burkholderia thailandensis E264 chromosome chromosome I, complete sequence.
Accession NC_007651
Length 3,809,201

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The map label for this gene is braF [H]

Identifier: 83721574

GI number: 83721574

Start: 50104

End: 50826

Strand: Reverse

Name: braF [H]

Synonym: BTH_I0046

Alternate gene names: 83721574

Gene position: 50826-50104 (Counterclockwise)

Preceding gene: 83720544

Following gene: 83719476

Centisome position: 1.33

GC content: 70.95

Gene sequence:

>723_bases
ATGATCGAGATCTCGAACCTGACCGTGCAGTTCGGCGGCACGCGCGTGCTCGATGCGCTCGACGCGAAGCTCGCCGCGCC
GATATGCGGGCTGATCGGCCCGAACGGCGCGGGCAAGACGACGCTCCTGAACGTGCTGAGCGGCTTTCTGCGGCCGCGCG
CGGGCAGCGTCGCGCTCGACGGCCGCGCGCTGCTCGCGCTCTCCGTCGCCGAGCGCGTGCGCGCCGGCGTGCGGCGCACG
TTCCAGACCGAACAGATCGTCGAGGATCTGAGCGTTCACGACAACGTGCTCGCGCTTGCCGAGCACGTGATGCCGGCCTT
CGCCGCGCGCGAGGACACGCGGCGCGCGCTCGAGCTTGTCGGCCTCGCGGATGTCGCGCACATGCGCGGCGCGGCCCTCA
ACCTGTATCAGCGCCGGATGCTGGAGCTCGGCAAGGCGCTCGTCGGCGCGCCGCGCCTGCTGCTGCTCGACGAGCCGGGC
GCCGGCCTGAACGAGACGGAAGCCGCGCGGCTCGCCGACGTGATCGTTCGCATTCCGGAATGTGTCGGCGCGCAGGTGCT
GCTGATCGATCACGACGTCGACCTGATCGACGCCGTGTGCGAGGCGACGCTCGTGCTCGATTTCGGCAAGCGGCTCGCGC
TCGGTCCGACACGCGCGGTGCTCGACGATCCGCTCGTGCGCAGCGCGTATCTCGGCCAGGCGTTCGACGAGCATGCGGCC
TGA

Upstream 100 bases:

>100_bases
TGCATGCGCTGATCACGGCGCCCGCCGGCATCGCCGGGCAGCTTGCGGCGCTCGCGTCGCGGATCGCGCGCGCGTTTCGC
GCCCGCGACGGAGGCGCACG

Downstream 100 bases:

>100_bases
CGCACGCGGCGCGGCATGCGCGCCGCATTCCATTCGACAAGGAGTCCGACGATCATGCGGCTCGAAGTGAAAGACCTCGT
CGTTCATCGCGCGAACAAGC

Product: branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALDGRALLALSVAERVRAGVRRT
FQTEQIVEDLSVHDNVLALAEHVMPAFAAREDTRRALELVGLADVAHMRGAALNLYQRRMLELGKALVGAPRLLLLDEPG
AGLNETEAARLADVIVRIPECVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHAA

Sequences:

>Translated_240_residues
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALDGRALLALSVAERVRAGVRRT
FQTEQIVEDLSVHDNVLALAEHVMPAFAAREDTRRALELVGLADVAHMRGAALNLYQRRMLELGKALVGAPRLLLLDEPG
AGLNETEAARLADVIVRIPECVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHAA
>Mature_240_residues
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALDGRALLALSVAERVRAGVRRT
FQTEQIVEDLSVHDNVLALAEHVMPAFAAREDTRRALELVGLADVAHMRGAALNLYQRRMLELGKALVGAPRLLLLDEPG
AGLNETEAARLADVIVRIPECVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHAA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=192, Percent_Identity=29.1666666666667, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI27881501, Length=212, Percent_Identity=25, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI30795238, Length=212, Percent_Identity=25, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI27477115, Length=181, Percent_Identity=29.8342541436464, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI153792144, Length=170, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI116734710, Length=191, Percent_Identity=26.1780104712042, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789864, Length=252, Percent_Identity=33.7301587301587, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789593, Length=235, Percent_Identity=32.3404255319149, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1786398, Length=243, Percent_Identity=30.4526748971193, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786563, Length=261, Percent_Identity=30.6513409961686, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081791, Length=231, Percent_Identity=27.7056277056277, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788472, Length=232, Percent_Identity=26.2931034482759, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081709, Length=238, Percent_Identity=25.2100840336134, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789991, Length=234, Percent_Identity=28.6324786324786, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082268, Length=238, Percent_Identity=28.1512605042017, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788761, Length=216, Percent_Identity=28.7037037037037, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787029, Length=228, Percent_Identity=27.1929824561404, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790525, Length=236, Percent_Identity=25.4237288135593, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789586, Length=231, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789873, Length=220, Percent_Identity=30.9090909090909, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786872, Length=228, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1790544, Length=249, Percent_Identity=28.9156626506024, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI48995001, Length=244, Percent_Identity=26.2295081967213, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787712, Length=196, Percent_Identity=27.0408163265306, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI48994997, Length=243, Percent_Identity=27.9835390946502, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081782, Length=198, Percent_Identity=31.3131313131313, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788225, Length=234, Percent_Identity=26.0683760683761, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87081834, Length=178, Percent_Identity=28.6516853932584, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1787792, Length=223, Percent_Identity=25.5605381165919, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788897, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787370, Length=237, Percent_Identity=23.6286919831224, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787164, Length=197, Percent_Identity=35.0253807106599, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788450, Length=229, Percent_Identity=26.2008733624454, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367384, Length=217, Percent_Identity=26.7281105990783, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788506, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1786345, Length=221, Percent_Identity=26.6968325791855, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789032, Length=222, Percent_Identity=26.5765765765766, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48994943, Length=226, Percent_Identity=24.3362831858407, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787548, Length=252, Percent_Identity=28.1746031746032, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790739, Length=238, Percent_Identity=27.3109243697479, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788210, Length=190, Percent_Identity=27.8947368421053, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789672, Length=214, Percent_Identity=23.8317757009346, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787041, Length=238, Percent_Identity=26.890756302521, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790467, Length=218, Percent_Identity=25.6880733944954, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI48994883, Length=251, Percent_Identity=24.7011952191235, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787758, Length=206, Percent_Identity=28.1553398058252, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1786253, Length=203, Percent_Identity=26.1083743842365, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1789891, Length=188, Percent_Identity=27.1276595744681, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1787500, Length=211, Percent_Identity=23.696682464455, Blast_Score=60, Evalue=9e-11,
Organism=Escherichia coli, GI1790190, Length=205, Percent_Identity=23.9024390243902, Blast_Score=60, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17510237, Length=228, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=220, Percent_Identity=28.1818181818182, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=220, Percent_Identity=28.1818181818182, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=220, Percent_Identity=28.1818181818182, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=211, Percent_Identity=30.8056872037915, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=211, Percent_Identity=25.1184834123223, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533592, Length=178, Percent_Identity=30.3370786516854, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI221512771, Length=212, Percent_Identity=25, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24643674, Length=200, Percent_Identity=25.5, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25534; Mature: 25534

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALD
CEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEC
GRALLALSVAERVRAGVRRTFQTEQIVEDLSVHDNVLALAEHVMPAFAAREDTRRALELV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLADVAHMRGAALNLYQRRMLELGKALVGAPRLLLLDEPGAGLNETEAARLADVIVRIPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
CVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHAA
HCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALD
CEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEC
GRALLALSVAERVRAGVRRTFQTEQIVEDLSVHDNVLALAEHVMPAFAAREDTRRALELV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLADVAHMRGAALNLYQRRMLELGKALVGAPRLLLLDEPGAGLNETEAARLADVIVRIPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
CVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHAA
HCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]