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The map label for this gene is cysT [H]

Identifier: 83721508

GI number: 83721508

Start: 2837568

End: 2838488

Strand: Reverse

Name: cysT [H]

Synonym: BTH_I2479

Alternate gene names: 83721508

Gene position: 2838488-2837568 (Counterclockwise)

Preceding gene: 83721122

Following gene: 83719584

Centisome position: 74.52

GC content: 69.16

Gene sequence:

>921_bases
GTGCATCACCCTGGAAGAGCATTCCGCATGACGACGTTCACCTTCCGCAAGCCGAGCGCGCTGCCCGGATTCGGCGTGAC
GCTCGGCATCACGCTGGCCTATCTGAGCCTCGTGGTGCTGATCCCGCTCGCCGCCACCTTTCTGAAGACGGCCACGCTCA
CGTGGGACCAGTTCGTCGCCGCGACGACGTCGCCGCGCGTGCTCGCGTCGTACCGGCTCACGTTTGCGGCCGCGCTGGGC
GGCGCGCTCGTGAACGCGGTGTTCGGCTTTCTCGTCGCGTGGGTGCTCGTGCGCTACACGTTCCCGCTCAAGCGGCTCGT
CGACGCGATCGTCGATTTGCCGTTCGCGCTGCCGACGTCGGTCGCGGGCATTTCGCTCGCGGCCGTGTACGCGCAGAACG
GCTGGATCGGCAGCCGTCTCGCGCCGCTCGGCATCAAGATCGCGTTCACGCCGCTCGGCGTGCTCGTCGCGCTCACGTTC
ATCGGCCTGCCGTTCGTCGTGCGCACCGTGCAGCCCGTTCTCGAGGATTTCGAGCGCGAGCAGGAGGAGGCGGCCGCGTG
CCTCGGCGCGTCGCGCTGGCTCACGTTCCGCCGCGTCGTGCTGCCCGCCGTGACGCCTGCGCTGCTCACGGGCTTCGCGC
TCGCGTTCGCGCGCGCGCTCGGCGAATACGGCTCGGTGATCTTCATCGCCGGCAACGTGCCGATGAAGTCGGAAATCACG
TCGCTGCTCATCATCACGAAGCTCGAGCAGTACGACTACGCAGGCGCGACCGCGCTCGCCGTCGTGATGCTCGTCGTGTC
GTTCGCGATGCTGCTCCTCATCAACACGCTGCAGTGGTACCTACAGCGCCGCACGAGCCGCGGCGCGAGCGGCCCGGCGC
CGGCCGCGCACGCAGCGGCGGCGCTCGGAGGCACGCAATGA

Upstream 100 bases:

>100_bases
GCGTGTGCGGCGCGCCGCACGCGCGCGCCGTTTCGCGACAACACGATATCGGGTTCGGCGGACGCGCGTTGCAGCGCGTC
CGCCGAGCCGTTGAACCCAT

Downstream 100 bases:

>100_bases
GCCGCGATACCGTTCGATCCGTCGCGGCAGCCGCTCATGCCGACGCCGGCCGCGATTTCAGCCGTGAATCCGGCCGTGAA
TCCGTCCGTGAGGTGCGTCG

Product: sulfate ABC transporter permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MHHPGRAFRMTTFTFRKPSALPGFGVTLGITLAYLSLVVLIPLAATFLKTATLTWDQFVAATTSPRVLASYRLTFAAALG
GALVNAVFGFLVAWVLVRYTFPLKRLVDAIVDLPFALPTSVAGISLAAVYAQNGWIGSRLAPLGIKIAFTPLGVLVALTF
IGLPFVVRTVQPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVTPALLTGFALAFARALGEYGSVIFIAGNVPMKSEIT
SLLIITKLEQYDYAGATALAVVMLVVSFAMLLLINTLQWYLQRRTSRGASGPAPAAHAAAALGGTQ

Sequences:

>Translated_306_residues
MHHPGRAFRMTTFTFRKPSALPGFGVTLGITLAYLSLVVLIPLAATFLKTATLTWDQFVAATTSPRVLASYRLTFAAALG
GALVNAVFGFLVAWVLVRYTFPLKRLVDAIVDLPFALPTSVAGISLAAVYAQNGWIGSRLAPLGIKIAFTPLGVLVALTF
IGLPFVVRTVQPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVTPALLTGFALAFARALGEYGSVIFIAGNVPMKSEIT
SLLIITKLEQYDYAGATALAVVMLVVSFAMLLLINTLQWYLQRRTSRGASGPAPAAHAAAALGGTQ
>Mature_306_residues
MHHPGRAFRMTTFTFRKPSALPGFGVTLGITLAYLSLVVLIPLAATFLKTATLTWDQFVAATTSPRVLASYRLTFAAALG
GALVNAVFGFLVAWVLVRYTFPLKRLVDAIVDLPFALPTSVAGISLAAVYAQNGWIGSRLAPLGIKIAFTPLGVLVALTF
IGLPFVVRTVQPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVTPALLTGFALAFARALGEYGSVIFIAGNVPMKSEIT
SLLIITKLEQYDYAGATALAVVMLVVSFAMLLLINTLQWYLQRRTSRGASGPAPAAHAAAALGGTQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=264, Percent_Identity=50, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI87082099, Length=262, Percent_Identity=34.7328244274809, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786980, Length=202, Percent_Identity=34.6534653465347, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786254, Length=227, Percent_Identity=28.6343612334802, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787369, Length=155, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787368, Length=237, Percent_Identity=28.2700421940928, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32657; Mature: 32657

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHPGRAFRMTTFTFRKPSALPGFGVTLGITLAYLSLVVLIPLAATFLKTATLTWDQFVA
CCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ATTSPRVLASYRLTFAAALGGALVNAVFGFLVAWVLVRYTFPLKRLVDAIVDLPFALPTS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHH
VAGISLAAVYAQNGWIGSRLAPLGIKIAFTPLGVLVALTFIGLPFVVRTVQPVLEDFERE
HHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEAAACLGASRWLTFRRVVLPAVTPALLTGFALAFARALGEYGSVIFIAGNVPMKSEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
SLLIITKLEQYDYAGATALAVVMLVVSFAMLLLINTLQWYLQRRTSRGASGPAPAAHAAA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ALGGTQ
HCCCCC
>Mature Secondary Structure
MHHPGRAFRMTTFTFRKPSALPGFGVTLGITLAYLSLVVLIPLAATFLKTATLTWDQFVA
CCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ATTSPRVLASYRLTFAAALGGALVNAVFGFLVAWVLVRYTFPLKRLVDAIVDLPFALPTS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHH
VAGISLAAVYAQNGWIGSRLAPLGIKIAFTPLGVLVALTFIGLPFVVRTVQPVLEDFERE
HHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEAAACLGASRWLTFRRVVLPAVTPALLTGFALAFARALGEYGSVIFIAGNVPMKSEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
SLLIITKLEQYDYAGATALAVVMLVVSFAMLLLINTLQWYLQRRTSRGASGPAPAAHAAA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ALGGTQ
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]