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The map label for this gene is nagE [H]

Identifier: 83721414

GI number: 83721414

Start: 498280

End: 500049

Strand: Direct

Name: nagE [H]

Synonym: BTH_I0450

Alternate gene names: 83721414

Gene position: 498280-500049 (Clockwise)

Preceding gene: 83719492

Following gene: 83719687

Centisome position: 13.08

GC content: 69.6

Gene sequence:

>1770_bases
ATGGACGGGAATCCGTTTCTGAAAATACAGAGCCTCGGCAGGGCGCTGATGCTGCCGATCGCGGTGCTGCCGGTGGCGGG
CATCCTGCTGCGCCTCGGTCAGCAGGACGTGCTCAACATCAAGATGATCGCCGACGCGGGCGGCGCGATCTTCGACAACC
TGCCGCTGCTGTTCGCGATCGGCGTCGCGGTGGGCTTCGCGAAGGACAACAACGGCGTGGCGGCGCTCGCGGGCGCGATC
GGCTATCTGATCGAAGTCGCGATCATGAAGGACATCGATCCGAAGCTGAACATGGGCGTGCTGTCCGGGATCATCGCGGG
CGTCGTCGCGGGGCTGCTGTACAACCGCTACAAGGACATCAAGCTGCCCGACTACCTCGCGTTCTTCGGCGGCAAGCGCT
TCGTGCCGATCATCACGGGGCTCGCGTGCGTCGTGCTCGGGATCGTGTTCGGCTACGTATGGCAGCCGGTGCAGCACGCG
ATCGACGCGGTCGGCCAGTGGCTGCTGACGGCGGGCGCGATCGGCACGTTCGTCTACGGGTTCCTGAACCGTCTGCTGCT
CGTCACCGGGCTGCACCACATCATCAACTCGCTCGTCTGGTTCGTGTTCGGCACGTTCACGCCGGCGGCGGGCGGCGCGG
CGGTGACGGGCGATCTGCATCGCTTCTTCGCGGGCGATCCGAGCGCGGGCAGCTTCATGGCGGGCTTCTTCCCGATCATG
ATGTTCGGCCTGCCGGCCGCGTGTCTCGCGATGTTTCACGAGGCGCCCAAGGAGCGCCGCGCGATCGTCGGCGGCCTGCT
GTTCTCGATGGCGCTCACGTCGTTCCTGACGGGCGTGACCGAGCCGATCGAGTTCAGCTTCATGTTCCTCGCGCCGCTGC
TCTACGTGATCCACGCGGTGCTCACGGGCCTCTCGCTCGCGATCTGCCAACTGCTCGGCGTGAAGCTCGGCTTCACGTTC
TCGGCGGGCGCGATCGACTACGTGCTGAACTACGGGCTTTCGACGAAGGGCTGGATCGCGATCCCGCTCGGCCTCGCGTA
CGGTCTCGCCTACTACGGCCTGTTCCGCTTCTTCATCCGCAAGTTCAACATGGCGACGCCGGGCCGCGAGCCCGCGGGCG
CGGACGCGCAGGCGCAGTCGTTCGCATCGGGCGGCTTCGTCGCGCCGACGGCGGGCGCATCGGTGCCGCGCGCGCAGCGC
TACATCGCGGCGCTCGGCGGCGCGGCGAACCTGTCGGTGGTGGACGCGTGCACGACGCGGCTGCGTCTTTCCGTCGTCGA
TCCCGAGAAGGTGTCCGAAGCGGATCTGCGCACGATCGGCGCGCGCGGCGTGCTCAAGCGCGGCGGCAGCAGCGTGCAGG
TGATCATCGGGCCGGAGGCGGACCTCATCGCCGACGAGATTCGCGCGACGCTCGGCAGCGGCGCGGCGTCATCCTCGGCG
ACGGCTGCGCCTGCCGCGCCGGTTGCCGCGACGGCAACGGCCGCGGGCGCGCAGGCGGGCCCGCTCGATCCGGAACCGAC
GCGCTGGCTCGCGGTGTTCGGCGGCGCGACGAACGTCGCGTCGCTCGACGCGGTCGCGGCGACGCGTCTGCGCGTCGTCG
TGCGCGATCCGTCGGCGGTTGATCGCCAGCGCCTCGCGACGCTCGACGTCGCGTGGGTCGCGGGCGATACGTTCCACATC
GTTTGCGGTCCGTCGGCGCCGCGCTATGCGCAGCAGCTCGCGGCGCGCCTGCCTTCGCCGGACGGCGGCGCGGCGGCTCA
GCCCGCCTGA

Upstream 100 bases:

>100_bases
GTCCGCCGTCCGGCGAATCGCGCGCGTTTCAGACGCGTTTCAATCAACAGAGCTGATCAATTATTCAACGACGAGACACG
ATAAAGGACTGGAGGATCGA

Downstream 100 bases:

>100_bases
CGCGGCGACGCGGCCGGCCGAATCCGCGATGCGGGCTCGGCTTCGATCGGCCGGCATGGAAAACGGCTTGCGAATATCGC
AAGCCGTTTTTTCTTGGGCG

Product: PTS system N-acetylglucosamine-specific transporter subunit IIABC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQQDVLNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLACVVLGIVFGYVWQPVQHA
IDAVGQWLLTAGAIGTFVYGFLNRLLLVTGLHHIINSLVWFVFGTFTPAAGGAAVTGDLHRFFAGDPSAGSFMAGFFPIM
MFGLPAACLAMFHEAPKERRAIVGGLLFSMALTSFLTGVTEPIEFSFMFLAPLLYVIHAVLTGLSLAICQLLGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGLAYGLAYYGLFRFFIRKFNMATPGREPAGADAQAQSFASGGFVAPTAGASVPRAQR
YIAALGGAANLSVVDACTTRLRLSVVDPEKVSEADLRTIGARGVLKRGGSSVQVIIGPEADLIADEIRATLGSGAASSSA
TAAPAAPVAATATAAGAQAGPLDPEPTRWLAVFGGATNVASLDAVAATRLRVVVRDPSAVDRQRLATLDVAWVAGDTFHI
VCGPSAPRYAQQLAARLPSPDGGAAAQPA

Sequences:

>Translated_589_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQQDVLNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLACVVLGIVFGYVWQPVQHA
IDAVGQWLLTAGAIGTFVYGFLNRLLLVTGLHHIINSLVWFVFGTFTPAAGGAAVTGDLHRFFAGDPSAGSFMAGFFPIM
MFGLPAACLAMFHEAPKERRAIVGGLLFSMALTSFLTGVTEPIEFSFMFLAPLLYVIHAVLTGLSLAICQLLGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGLAYGLAYYGLFRFFIRKFNMATPGREPAGADAQAQSFASGGFVAPTAGASVPRAQR
YIAALGGAANLSVVDACTTRLRLSVVDPEKVSEADLRTIGARGVLKRGGSSVQVIIGPEADLIADEIRATLGSGAASSSA
TAAPAAPVAATATAAGAQAGPLDPEPTRWLAVFGGATNVASLDAVAATRLRVVVRDPSAVDRQRLATLDVAWVAGDTFHI
VCGPSAPRYAQQLAARLPSPDGGAAAQPA
>Mature_589_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQQDVLNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLACVVLGIVFGYVWQPVQHA
IDAVGQWLLTAGAIGTFVYGFLNRLLLVTGLHHIINSLVWFVFGTFTPAAGGAAVTGDLHRFFAGDPSAGSFMAGFFPIM
MFGLPAACLAMFHEAPKERRAIVGGLLFSMALTSFLTGVTEPIEFSFMFLAPLLYVIHAVLTGLSLAICQLLGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGLAYGLAYYGLFRFFIRKFNMATPGREPAGADAQAQSFASGGFVAPTAGASVPRAQR
YIAALGGAANLSVVDACTTRLRLSVVDPEKVSEADLRTIGARGVLKRGGSSVQVIIGPEADLIADEIRATLGSGAASSSA
TAAPAAPVAATATAAGAQAGPLDPEPTRWLAVFGGATNVASLDAVAATRLRVVVRDPSAVDRQRLATLDVAWVAGDTFHI
VCGPSAPRYAQQLAARLPSPDGGAAAQPA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=468, Percent_Identity=55.1282051282051, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1787343, Length=487, Percent_Identity=44.5585215605749, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787908, Length=515, Percent_Identity=36.6990291262136, Blast_Score=275, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61179; Mature: 61179

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHEEEEEECCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHEEEEEECCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1745234 [H]