Click here to switch to the map view.

The map label for this gene is tnpR [H]

Identifier: 83721411

GI number: 83721411

Start: 3573572

End: 3574225

Strand: Reverse

Name: tnpR [H]

Synonym: BTH_I3135

Alternate gene names: 83721411

Gene position: 3574225-3573572 (Counterclockwise)

Preceding gene: 83719431

Following gene: 83719644

Centisome position: 93.83

GC content: 59.63

Gene sequence:

>654_bases
GTGTCTATGGCGATCTTCGGGTACGGTCGGGTCAGCACGCGCGAACAGACAACCGAGAATCAGCGTCTCGAAATCGAGAA
TGCTGGATTCAAGGTCGATTACTGGTTCACGGACGAAGGCATCAGCGGCAAGACCCATGCGTCGCAGCGCCCGCAGTTCG
TCGCGATGTTGGACAAGATCAGGGATGGCGAGACGTTGGTTGTCTCCCGTCTCGACAGATTGGGACGCGATGCCGAAGAT
GTTCTGCGGACCGTGCGGACGTTGGCCGAACGCAAGATCGAAGTTATCGTGCTTCAACTCGGGAAACTCGATCTGGGGTC
ATCTGCCGGGAAGCTGATGCTCACGATGTTGTCTGCTGTTGCGGAAATGGAGCGTTCACTGCTGGTGGAGCGCACGCAAG
CCGGATTGGTGCGTGCGAAGGCAGAAGGCAAAACCCTTGGCAGACCGTCCAAGACAACGCCAGCGCAACGCGAAGAGATG
AAGGGCAAGTACGCGGCGGGTGAGACGGTAAGTGCCTTGGCGCGGACCTACGGCATCTCACGGGCGAGTGTGCTGGCCGT
GGTGAATCCGTCACGTGAACCGACTGGTTTCGGAGCGGACGGCGTACCGGTCGCACTCATCGATTGGGATCGTCGCAAGT
CGCGCGGCGCATGA

Upstream 100 bases:

>100_bases
AACAGTTATCGGATGTATTGGTCCGGCCTGTTGGAAATATAGGGTTGACTATTTCCGACATCAAGTGATATTCTGACACC
CTTTTCCAACGCCTTCGGGG

Downstream 100 bases:

>100_bases
ACATCAGCCGTCAAACCATGCGGATACGGGAATCGCAGTCGCGCAATCGGCGGCTGTCTCATAAGCCCGCTTCGGTCATC
AGCGCCGTCATCGTCATGCC

Product: resolvase TnpR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSMAIFGYGRVSTREQTTENQRLEIENAGFKVDYWFTDEGISGKTHASQRPQFVAMLDKIRDGETLVVSRLDRLGRDAED
VLRTVRTLAERKIEVIVLQLGKLDLGSSAGKLMLTMLSAVAEMERSLLVERTQAGLVRAKAEGKTLGRPSKTTPAQREEM
KGKYAAGETVSALARTYGISRASVLAVVNPSREPTGFGADGVPVALIDWDRRKSRGA

Sequences:

>Translated_217_residues
MSMAIFGYGRVSTREQTTENQRLEIENAGFKVDYWFTDEGISGKTHASQRPQFVAMLDKIRDGETLVVSRLDRLGRDAED
VLRTVRTLAERKIEVIVLQLGKLDLGSSAGKLMLTMLSAVAEMERSLLVERTQAGLVRAKAEGKTLGRPSKTTPAQREEM
KGKYAAGETVSALARTYGISRASVLAVVNPSREPTGFGADGVPVALIDWDRRKSRGA
>Mature_216_residues
SMAIFGYGRVSTREQTTENQRLEIENAGFKVDYWFTDEGISGKTHASQRPQFVAMLDKIRDGETLVVSRLDRLGRDAEDV
LRTVRTLAERKIEVIVLQLGKLDLGSSAGKLMLTMLSAVAEMERSLLVERTQAGLVRAKAEGKTLGRPSKTTPAQREEMK
GKYAAGETVSALARTYGISRASVLAVVNPSREPTGFGADGVPVALIDWDRRKSRGA

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787827, Length=182, Percent_Identity=46.1538461538462, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI1787638, Length=182, Percent_Identity=46.1538461538462, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787404, Length=175, Percent_Identity=37.7142857142857, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23787; Mature: 23655

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMAIFGYGRVSTREQTTENQRLEIENAGFKVDYWFTDEGISGKTHASQRPQFVAMLDKI
CCEEEEECCCCCCCHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
RDGETLVVSRLDRLGRDAEDVLRTVRTLAERKIEVIVLQLGKLDLGSSAGKLMLTMLSAV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHH
AEMERSLLVERTQAGLVRAKAEGKTLGRPSKTTPAQREEMKGKYAAGETVSALARTYGIS
HHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCC
RASVLAVVNPSREPTGFGADGVPVALIDWDRRKSRGA
CCEEEEEECCCCCCCCCCCCCCEEEEEECCCHHCCCC
>Mature Secondary Structure 
SMAIFGYGRVSTREQTTENQRLEIENAGFKVDYWFTDEGISGKTHASQRPQFVAMLDKI
CEEEEECCCCCCCHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
RDGETLVVSRLDRLGRDAEDVLRTVRTLAERKIEVIVLQLGKLDLGSSAGKLMLTMLSAV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHH
AEMERSLLVERTQAGLVRAKAEGKTLGRPSKTTPAQREEMKGKYAAGETVSALARTYGIS
HHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCC
RASVLAVVNPSREPTGFGADGVPVALIDWDRRKSRGA
CCEEEEEECCCCCCCCCCCCCCEEEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027041 [H]