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The map label for this gene is paaD [H]

Identifier: 83721405

GI number: 83721405

Start: 3338628

End: 3339092

Strand: Direct

Name: paaD [H]

Synonym: BTH_I2903

Alternate gene names: 83721405

Gene position: 3338628-3339092 (Clockwise)

Preceding gene: 83718849

Following gene: 83720428

Centisome position: 87.65

GC content: 64.95

Gene sequence:

>465_bases
ATGACCGAACGCTCTCCCACGGCAACCGCCGAACGCTCCGCCGAAGCGCTTGCCCGCGCGACCGCCGAAGCAATGTTTGC
CGCGGACGCCTGCAGCCGCGGCCTCGGCATCGAGCTGCTCGAAGTGCGGCCGGGCTATGCGCGCACGTGCATGCCGGTGC
GCCCGGATTTTCTGAACGGCCACCAGATCTGTCACGGCGGACTCATCTTCACGCTCGCGGATTCAACGTTCGCGTTCGCG
TGCAACTCGTACAACGTCAACACCGTCGCGGCCGGCTGCTCGATCGAATTCCTGCGGCCGGTGGCGGGCGGCGACGTGCT
CACCGCAGAGGCGACTGAGCAGACGCTGAACGGCCGGCACGGCATCTACGATATTCGCGTAACGAACCGGGCGGGAGAAA
CCGTCGCGATGTTCCGCGGCAAATCGACCCAAATCAAAGGGACGGTGATCCCTATAGACCGTTAG

Upstream 100 bases:

>100_bases
GAACGCGACCTTCAGCGCGAGCTCGGGCAATCGTACGACTACGCGGAGGGCGTGCGCGCGTTCATCGAAAAGCGCGCCCC
TCGCTTCGAAGGACGCTGAG

Downstream 100 bases:

>100_bases
CGTGCACACGCGCTCACATAAATATATTGGAGACATCATGACAACCTCGCTTCCGCTCGAACCGATCGAACGGGCGAGCC
GCGACGAACTGCTCGCGCTT

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTERSPTATAERSAEALARATAEAMFAADACSRGLGIELLEVRPGYARTCMPVRPDFLNGHQICHGGLIFTLADSTFAFA
CNSYNVNTVAAGCSIEFLRPVAGGDVLTAEATEQTLNGRHGIYDIRVTNRAGETVAMFRGKSTQIKGTVIPIDR

Sequences:

>Translated_154_residues
MTERSPTATAERSAEALARATAEAMFAADACSRGLGIELLEVRPGYARTCMPVRPDFLNGHQICHGGLIFTLADSTFAFA
CNSYNVNTVAAGCSIEFLRPVAGGDVLTAEATEQTLNGRHGIYDIRVTNRAGETVAMFRGKSTQIKGTVIPIDR
>Mature_153_residues
TERSPTATAERSAEALARATAEAMFAADACSRGLGIELLEVRPGYARTCMPVRPDFLNGHQICHGGLIFTLADSTFAFAC
NSYNVNTVAAGCSIEFLRPVAGGDVLTAEATEQTLNGRHGIYDIRVTNRAGETVAMFRGKSTQIKGTVIPIDR

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=132, Percent_Identity=47.7272727272727, Blast_Score=136, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 16441; Mature: 16310

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERSPTATAERSAEALARATAEAMFAADACSRGLGIELLEVRPGYARTCMPVRPDFLNG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCC
HQICHGGLIFTLADSTFAFACNSYNVNTVAAGCSIEFLRPVAGGDVLTAEATEQTLNGRH
CEEECCCEEEEEECCEEEEEECCCCCEEEEECCEEEEECCCCCCCEEEECCHHHHCCCCC
GIYDIRVTNRAGETVAMFRGKSTQIKGTVIPIDR
CEEEEEEECCCCCEEEEEECCCCEEEEEEEECCC
>Mature Secondary Structure 
TERSPTATAERSAEALARATAEAMFAADACSRGLGIELLEVRPGYARTCMPVRPDFLNG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCC
HQICHGGLIFTLADSTFAFACNSYNVNTVAAGCSIEFLRPVAGGDVLTAEATEQTLNGRH
CEEECCCEEEEEECCEEEEEECCCCCEEEEECCEEEEECCCCCCCEEEECCHHHHCCCCC
GIYDIRVTNRAGETVAMFRGKSTQIKGTVIPIDR
CEEEEEEECCCCCEEEEEECCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]