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The map label for this gene is pit [H]

Identifier: 83721392

GI number: 83721392

Start: 2457988

End: 2459052

Strand: Direct

Name: pit [H]

Synonym: BTH_I2185

Alternate gene names: 83721392

Gene position: 2457988-2459052 (Clockwise)

Preceding gene: 83720511

Following gene: 83721092

Centisome position: 64.53

GC content: 63.47

Gene sequence:

>1065_bases
ATGTCGCGAACATCATCGAAGGCATCGTGCTGGAAAACGCCTGAGCGGACCACGATGCATTCGATACAACTCGCCTTGTG
GGTCGTCGCGGCACTCGTGCTTGTCGCGCTCGTGTTCGATTTCATGAACGGCTTTCACGACGCCGCGAACTCGATCGCGA
CCGTCGTGTCGACCGGGGTGCTGAAGCCCCAGCAAGCCGTCGTGTTCGCGGCGGCATTCAACGTCATCGCGTATTTCATC
TTCCATCTGAAAGTTGCGCAGACCGTCGGCAAGGGCACGATCGACGCGAGCATCGTCGATCACTACGTCGTGTTCGGCGC
GCTGTTCGGTGCGATCGGCTGGAACATCATTACGTGGTACTACGGGATTCCGTCGAGCTCGTCGCACGCGCTGATCGGCG
GGCTCGTCGGCGCCGCGGTGTCGAAGTCGGGCTGGGGATCGCTCAACGTCGACGGGCTGATGAAGACGGTCGCATTCATC
TTCATCTCGCCGCTTCTCGGCTTCATCCTCGGATCGCTGTTCATGCTCGGGGTATCGTGGCTGTACTTCCGGACCGCGCC
AAGCAAGGTCGACCGGCGTTTCAGGCGGCTGCAACTGTTGTCGGCCAGCCTCTACAGCCTCGGCCACGGCGGCAACGACG
CGCAGAAGACAATCGGCATCATTTGGATGCTGCTGATCGCGTCCGGGTACGCATCGGCCGCCTCCGACGCGCCGCCTGCG
TGGGTGATCGGCGCGTGCTATCTGTCGATGGGCCTCGGCACGTTGTTCGGCGGTTGGCGGATCGTGCGCACGATGGGTCA
GAAGATCACGAAGCTCAAGCCCGTGGGCGGCTTCTGCGCGGAGACAGGCGGCGCGCTCACTCTGTTCATCGCGTCGTGGA
TGGGTATCCCGGTGTCGACCACGCATACGATCACGGGTGCGATCGTCGGCGTCGGCGCGACGCGCAAGCTGTCGGCCGTA
CGGTGGGGCGTCGCAGGCAACATCGTGTGGGCATGGGTGCTGACGATTCCCGCATCGGCATTGATCGCGGCGGCCGGGTG
GTGGATCGGGCACCGCGTGTTCTGA

Upstream 100 bases:

>100_bases
GCGATGTCGAAGCTCTTCCGCGAGGAAGACGACGTGAAGACGCTCATCAAGCTGAAGGCGATCTACGAGCTGCTCGAGCA
GATCACCGACAAGTGCGAGG

Downstream 100 bases:

>100_bases
TGCGCTGAGCGCGGCGATCGCGTGACGCAACGCCGCGTCTCGCCGTGCGGAAAAAAACGATGCGCCGCATGCCGACAGGC
TGCGGCGCATTGTCTTTTCC

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSRTSSKASCWKTPERTTMHSIQLALWVVAALVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFI
FHLKVAQTVGKGTIDASIVDHYVVFGALFGAIGWNIITWYYGIPSSSSHALIGGLVGAAVSKSGWGSLNVDGLMKTVAFI
FISPLLGFILGSLFMLGVSWLYFRTAPSKVDRRFRRLQLLSASLYSLGHGGNDAQKTIGIIWMLLIASGYASAASDAPPA
WVIGACYLSMGLGTLFGGWRIVRTMGQKITKLKPVGGFCAETGGALTLFIASWMGIPVSTTHTITGAIVGVGATRKLSAV
RWGVAGNIVWAWVLTIPASALIAAAGWWIGHRVF

Sequences:

>Translated_354_residues
MSRTSSKASCWKTPERTTMHSIQLALWVVAALVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFI
FHLKVAQTVGKGTIDASIVDHYVVFGALFGAIGWNIITWYYGIPSSSSHALIGGLVGAAVSKSGWGSLNVDGLMKTVAFI
FISPLLGFILGSLFMLGVSWLYFRTAPSKVDRRFRRLQLLSASLYSLGHGGNDAQKTIGIIWMLLIASGYASAASDAPPA
WVIGACYLSMGLGTLFGGWRIVRTMGQKITKLKPVGGFCAETGGALTLFIASWMGIPVSTTHTITGAIVGVGATRKLSAV
RWGVAGNIVWAWVLTIPASALIAAAGWWIGHRVF
>Mature_353_residues
SRTSSKASCWKTPERTTMHSIQLALWVVAALVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIF
HLKVAQTVGKGTIDASIVDHYVVFGALFGAIGWNIITWYYGIPSSSSHALIGGLVGAAVSKSGWGSLNVDGLMKTVAFIF
ISPLLGFILGSLFMLGVSWLYFRTAPSKVDRRFRRLQLLSASLYSLGHGGNDAQKTIGIIWMLLIASGYASAASDAPPAW
VIGACYLSMGLGTLFGGWRIVRTMGQKITKLKPVGGFCAETGGALTLFIASWMGIPVSTTHTITGAIVGVGATRKLSAVR
WGVAGNIVWAWVLTIPASALIAAAGWWIGHRVF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=169, Percent_Identity=38.4615384615385, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI5803173, Length=159, Percent_Identity=38.3647798742138, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789360, Length=225, Percent_Identity=32, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=30.4147465437788, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17539280, Length=174, Percent_Identity=32.7586206896552, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=30.8641975308642, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=29.0123456790123, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32566716, Length=169, Percent_Identity=30.1775147928994, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17536725, Length=169, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557328, Length=155, Percent_Identity=34.8387096774194, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319773, Length=172, Percent_Identity=30.2325581395349, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356511, Length=182, Percent_Identity=30.2197802197802, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 37635; Mature: 37503

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRTSSKASCWKTPERTTMHSIQLALWVVAALVLVALVFDFMNGFHDAANSIATVVSTGV
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC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CHHHHHHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]