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The map label for this gene is fabG2 [H]

Identifier: 83721117

GI number: 83721117

Start: 848169

End: 848927

Strand: Reverse

Name: fabG2 [H]

Synonym: BTH_I0738

Alternate gene names: 83721117

Gene position: 848927-848169 (Counterclockwise)

Preceding gene: 83719561

Following gene: 83719209

Centisome position: 22.29

GC content: 70.22

Gene sequence:

>759_bases
ATGGAGATTCGCGACAACGTCTTCCTGATCACGGGCGGCGCATCCGGGCTCGGAGCGGGCACCGCGCGCCTGCTGACCGA
AGCAGGCGGCAAGGTCGTGCTCGCGGACCTGAATCAGGACGCGGGCGAAGCGCTCGCGCGCGAGCTGGGCGGCGTGTTCG
TCCGGTGCGACGTCGCGCGCGAGGAAGACGCGCAGGCGGCCGTCGCCGCCGCGACGAAGCTCGGCACGCTGCGCGGGCTC
GTCAACTGCGCGGGCATCGCGCCCGCGGCGAAAACGGTCGGCAAGGACGGCCCGCACCCGCTCGAGCTGTTCGCGAAGAC
GATCACCGTGAACCTGATCGGCACGTTCAACATGATTCGCGTCGCGGCCGCTGCGATGGCCGCGAACGAGCCGGCGCCAA
CCGGCGAGCGCGGCGTGATCGTCAGCACCGCGTCGGTCGCCGCGTTCGACGGCCAGATCGGCCAGGCCGCGTACGCGGCG
TCGAAGGCGGGCGTCGCCGGCATGACGCTGCCCATCGCGCGCGACCTGTCGCGCAATGCGATTCGCGTGATGACGATCGC
GCCCGGCATCTTCGAAACGCCGATGCTGCTCGGCATGCCGCAGGAAGTGCAGGACGCGCTCGGCGCGATGGTGCCGTTCC
CGCCGCGCCTCGGCAAGCCGGCCGAATACGCGATGCTCGTCAGGCAGATCTTCGAGAACCCGATGCTCAACGGCGAGGTC
ATCCGCCTAGACGGCGCGATTCGGATGCAGCCGAAGTAA

Upstream 100 bases:

>100_bases
CGCGCCGGGCTTCCGCGCTCGCTCGTACAATCGTCGCAACGCGCGCTTCACGACATTCGATCGACATTCATTCCAATCAA
TCCAAGGAGAAGGAGCAAAC

Downstream 100 bases:

>100_bases
TGCGGCGGCGCACGCGGCCACAGCGGCGCCGCGTGCAGCACACCGCCGAGCGGACAAAAAACGCCTGCGGAATGCAGCGG
CATCCGCAGGCGTCTTCATG

Product: 3-hydroxyacyl-CoA dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MEIRDNVFLITGGASGLGAGTARLLTEAGGKVVLADLNQDAGEALARELGGVFVRCDVAREEDAQAAVAAATKLGTLRGL
VNCAGIAPAAKTVGKDGPHPLELFAKTITVNLIGTFNMIRVAAAAMAANEPAPTGERGVIVSTASVAAFDGQIGQAAYAA
SKAGVAGMTLPIARDLSRNAIRVMTIAPGIFETPMLLGMPQEVQDALGAMVPFPPRLGKPAEYAMLVRQIFENPMLNGEV
IRLDGAIRMQPK

Sequences:

>Translated_252_residues
MEIRDNVFLITGGASGLGAGTARLLTEAGGKVVLADLNQDAGEALARELGGVFVRCDVAREEDAQAAVAAATKLGTLRGL
VNCAGIAPAAKTVGKDGPHPLELFAKTITVNLIGTFNMIRVAAAAMAANEPAPTGERGVIVSTASVAAFDGQIGQAAYAA
SKAGVAGMTLPIARDLSRNAIRVMTIAPGIFETPMLLGMPQEVQDALGAMVPFPPRLGKPAEYAMLVRQIFENPMLNGEV
IRLDGAIRMQPK
>Mature_252_residues
MEIRDNVFLITGGASGLGAGTARLLTEAGGKVVLADLNQDAGEALARELGGVFVRCDVAREEDAQAAVAAATKLGTLRGL
VNCAGIAPAAKTVGKDGPHPLELFAKTITVNLIGTFNMIRVAAAAMAANEPAPTGERGVIVSTASVAAFDGQIGQAAYAA
SKAGVAGMTLPIARDLSRNAIRVMTIAPGIFETPMLLGMPQEVQDALGAMVPFPPRLGKPAEYAMLVRQIFENPMLNGEV
IRLDGAIRMQPK

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=255, Percent_Identity=58.0392156862745, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI83715985, Length=256, Percent_Identity=55.46875, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=30.4, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI15277342, Length=240, Percent_Identity=34.1666666666667, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI59889578, Length=219, Percent_Identity=28.7671232876712, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI31542939, Length=261, Percent_Identity=27.2030651340996, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI32483357, Length=234, Percent_Identity=30.3418803418803, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5031737, Length=195, Percent_Identity=29.2307692307692, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787905, Length=227, Percent_Identity=32.15859030837, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787335, Length=257, Percent_Identity=28.7937743190661, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082100, Length=233, Percent_Identity=30.4721030042918, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082160, Length=196, Percent_Identity=31.6326530612245, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=246, Percent_Identity=50.8130081300813, Blast_Score=240, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=30.5220883534137, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17555706, Length=222, Percent_Identity=33.7837837837838, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17563726, Length=211, Percent_Identity=28.9099526066351, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=204, Percent_Identity=29.9019607843137, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737361, Length=254, Percent_Identity=58.2677165354331, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=32.8063241106719, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21357041, Length=229, Percent_Identity=29.2576419213974, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571526, Length=229, Percent_Identity=27.9475982532751, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 26003; Mature: 26003

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRDNVFLITGGASGLGAGTARLLTEAGGKVVLADLNQDAGEALARELGGVFVRCDVAR
CCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEEECCC
EEDAQAAVAAATKLGTLRGLVNCAGIAPAAKTVGKDGPHPLELFAKTITVNLIGTFNMIR
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHEEEEHHHHHHHH
VAAAAMAANEPAPTGERGVIVSTASVAAFDGQIGQAAYAASKAGVAGMTLPIARDLSRNA
HHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCHHHHHHHHCCCCCEEHHHHHHCCCCC
IRVMTIAPGIFETPMLLGMPQEVQDALGAMVPFPPRLGKPAEYAMLVRQIFENPMLNGEV
EEEEEECCCHHHCCHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
IRLDGAIRMQPK
EEECCEEEECCC
>Mature Secondary Structure
MEIRDNVFLITGGASGLGAGTARLLTEAGGKVVLADLNQDAGEALARELGGVFVRCDVAR
CCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEEECCC
EEDAQAAVAAATKLGTLRGLVNCAGIAPAAKTVGKDGPHPLELFAKTITVNLIGTFNMIR
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHEEEEHHHHHHHH
VAAAAMAANEPAPTGERGVIVSTASVAAFDGQIGQAAYAASKAGVAGMTLPIARDLSRNA
HHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCHHHHHHHHCCCCCEEHHHHHHCCCCC
IRVMTIAPGIFETPMLLGMPQEVQDALGAMVPFPPRLGKPAEYAMLVRQIFENPMLNGEV
EEEEEECCCHHHCCHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
IRLDGAIRMQPK
EEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]