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The map label for this gene is rnfF [H]

Identifier: 83721093

GI number: 83721093

Start: 2617366

End: 2618424

Strand: Direct

Name: rnfF [H]

Synonym: BTH_I2326

Alternate gene names: 83721093

Gene position: 2617366-2618424 (Clockwise)

Preceding gene: 83719777

Following gene: 83720810

Centisome position: 68.71

GC content: 72.9

Gene sequence:

>1059_bases
TTGTCCGGTTTCAAGAAACTCGTCGGATCGTCGCTCGCGCTCGCCGCCGTCATCGCATTGACGCTCGGGCTCGCGCTGAG
GGCCCCGCAGGTATACGTGCAGGGCACGTACGTGTTCGGCACGCGCGTGCAGCTCGCGATCTACGGCGTGCCGCTCGATC
GCGCGCAGCAGGCGACGAACGCGGTGTTCGCGCACTTCCAGGCGATGCAGCGCGATCTGCACGCATGGCGGCCGTCGGAG
ATCACGCGCCTTAACCGAAGCATCGCCGCGGGCGAGCCGTTTCGCGCATCGCCCGCGACGGCCGAGATCCTGCGCGCGGC
GCGCAGGCTGTCGCTCGACACGCAGGGGCTCTTCGAGCCCGGCATCGGGCGGCTCATTCGCCTCTGGGGCTTCCAGTCCG
ATCAGTTTCGCGTCGCGCCGCCGTCGCGCGACGCGGTGCGGCGCGAGGTCGCGCGCGGCGCGCGCATCGCCGATCTCGCG
ATTTCGGCCGACGGCGTCGTGACGAGCGCGAACCGCGCGGTCGCGGTCGATCTTGGCGGCTTCGCGAAGGGCTGGGCGCT
CGACGATGCGGCCGCGATCCTCAGGAAGCAGGGGATCGGCAATGCGCTGATCGACGTCGGCGGCAATCTGCTCGCGCTCG
GCAGCAAGGGCGGCGCGGCGTGGCGCGTCGGCGTGCAGGACCCGCGCAAGCCCGGCACGCTCGCGACGCTCGAGCTGCGC
GACGGCGAGGCGATCGGCACGAGCGGCGATTACGAGCGCTTCTTCCAGGCGGACGGCGTGCGCTACTGCCACATCCTCGA
TCCGCGCAGCGGCTTTCCCGCCGCGCGAAGCGAGGCCGCGACGGTGCTCGTCGCGCCCGGCCCGCACGCGGGCGCGCTGT
CCGACGGCGCGAGCAAGCCGCCGTTCATCGCGGGGCCCGCGGCGATGCCGCTCACGCGCGGGCTCGGCGTCGAAGCGGTG
CTGATCGTCGATGCGCAAGGGCGCGTGTGGGCGACCGACGCGATGGCCGCGCGCGTGCGCTTCGCCGATCCGGCGCTGCG
CGCCGCGCGGCTCGATTGA

Upstream 100 bases:

>100_bases
CACGCAGCCGACCGATCCGAAAAAACGATAATCGTTCGGTGTCCGGATGTCGTCCGGGCAGCGATGTGCATGACGCCAAT
CGTTTTCGGAAATTCAATCC

Downstream 100 bases:

>100_bases
ACGACGGAGCGCCGCCGCGATGACGAACGGACCACATGGACTACATGGACCGCACGGACCGCACGGACCGTGCATCGACG
TGCAGGACGTGAGCCTCCAT

Product: ApbE family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSGFKKLVGSSLALAAVIALTLGLALRAPQVYVQGTYVFGTRVQLAIYGVPLDRAQQATNAVFAHFQAMQRDLHAWRPSE
ITRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEPGIGRLIRLWGFQSDQFRVAPPSRDAVRREVARGARIADLA
ISADGVVTSANRAVAVDLGGFAKGWALDDAAAILRKQGIGNALIDVGGNLLALGSKGGAAWRVGVQDPRKPGTLATLELR
DGEAIGTSGDYERFFQADGVRYCHILDPRSGFPAARSEAATVLVAPGPHAGALSDGASKPPFIAGPAAMPLTRGLGVEAV
LIVDAQGRVWATDAMAARVRFADPALRAARLD

Sequences:

>Translated_352_residues
MSGFKKLVGSSLALAAVIALTLGLALRAPQVYVQGTYVFGTRVQLAIYGVPLDRAQQATNAVFAHFQAMQRDLHAWRPSE
ITRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEPGIGRLIRLWGFQSDQFRVAPPSRDAVRREVARGARIADLA
ISADGVVTSANRAVAVDLGGFAKGWALDDAAAILRKQGIGNALIDVGGNLLALGSKGGAAWRVGVQDPRKPGTLATLELR
DGEAIGTSGDYERFFQADGVRYCHILDPRSGFPAARSEAATVLVAPGPHAGALSDGASKPPFIAGPAAMPLTRGLGVEAV
LIVDAQGRVWATDAMAARVRFADPALRAARLD
>Mature_351_residues
SGFKKLVGSSLALAAVIALTLGLALRAPQVYVQGTYVFGTRVQLAIYGVPLDRAQQATNAVFAHFQAMQRDLHAWRPSEI
TRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEPGIGRLIRLWGFQSDQFRVAPPSRDAVRREVARGARIADLAI
SADGVVTSANRAVAVDLGGFAKGWALDDAAAILRKQGIGNALIDVGGNLLALGSKGGAAWRVGVQDPRKPGTLATLELRD
GEAIGTSGDYERFFQADGVRYCHILDPRSGFPAARSEAATVLVAPGPHAGALSDGASKPPFIAGPAAMPLTRGLGVEAVL
IVDAQGRVWATDAMAARVRFADPALRAARLD

Specific function: Required for nitrogen fixation [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rseC family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=266, Percent_Identity=27.4436090225564, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007359
- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE; PF04246 RseC_MucC [H]

EC number: NA

Molecular weight: Translated: 37074; Mature: 36943

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFKKLVGSSLALAAVIALTLGLALRAPQVYVQGTYVFGTRVQLAIYGVPLDRAQQATN
CCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECEEEEEEEECCHHHHHHHHH
AVFAHFQAMQRDLHAWRPSEITRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
GIGRLIRLWGFQSDQFRVAPPSRDAVRREVARGARIADLAISADGVVTSANRAVAVDLGG
CHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCEEEEEEEECCCEEEECCCEEEEECCC
FAKGWALDDAAAILRKQGIGNALIDVGGNLLALGSKGGAAWRVGVQDPRKPGTLATLELR
CCCCCCCHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEEC
DGEAIGTSGDYERFFQADGVRYCHILDPRSGFPAARSEAATVLVAPGPHAGALSDGASKP
CCCEECCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
PFIAGPAAMPLTRGLGVEAVLIVDAQGRVWATDAMAARVRFADPALRAARLD
CEEECCCCCCHHCCCCCEEEEEEECCCCEEECHHHHHEEEECCHHHHHHCCC
>Mature Secondary Structure 
SGFKKLVGSSLALAAVIALTLGLALRAPQVYVQGTYVFGTRVQLAIYGVPLDRAQQATN
CHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECEEEEEEEECCHHHHHHHHH
AVFAHFQAMQRDLHAWRPSEITRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
GIGRLIRLWGFQSDQFRVAPPSRDAVRREVARGARIADLAISADGVVTSANRAVAVDLGG
CHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCEEEEEEEECCCEEEECCCEEEEECCC
FAKGWALDDAAAILRKQGIGNALIDVGGNLLALGSKGGAAWRVGVQDPRKPGTLATLELR
CCCCCCCHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEEC
DGEAIGTSGDYERFFQADGVRYCHILDPRSGFPAARSEAATVLVAPGPHAGALSDGASKP
CCCEECCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
PFIAGPAAMPLTRGLGVEAVLIVDAQGRVWATDAMAARVRFADPALRAARLD
CEEECCCCCCHHCCCCCEEEEEEECCCCEEECHHHHHEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 2071578 [H]