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The map label for this gene is pncA [H]

Identifier: 83721021

GI number: 83721021

Start: 2422601

End: 2423239

Strand: Direct

Name: pncA [H]

Synonym: BTH_I2143

Alternate gene names: 83721021

Gene position: 2422601-2423239 (Clockwise)

Preceding gene: 83721623

Following gene: 83718481

Centisome position: 63.6

GC content: 71.36

Gene sequence:

>639_bases
ATGCAGCGCACCGACGATGTCCTTCTCGTGATCGACGTGCAGAACGATTTCATGCCGGGCGGCGCGCTCGCGGTTGCGCA
CGGCGACGAAATCGTGCCGGTGATCAACCGGCTCGCCGCGCGCTTCGATCACGTCGTGCTGACGCAGGACTGGCATCCGC
GCGATCACGTGTCGTTCGCGGCGAACCATCCGGGCCGCGCGCCGTTCTCGACCGTCGAGCTGCCGTACGGCGAGCAGGTG
CTGTGGCCCGTGCATTGCGTGCAGGGCACGCAAGGCGCGGCGCTGCACGGCGATCTCGACATTCCGCACGCGCGGCTCGT
GATCCGCAAGGGTCATGACGCGTCGGTCGACAGCTACTCGGCGTTCGTCGAAGCCGATCGCACGACGCCGACGGGGCTTG
CCGGCTATCTGCGCGAGCTCGGCGCGAAGCGCGTGTGGTGCTGCGGGCTCGCGACGGACTATTGCGTCGCGTGGTCCGCG
CTCGACGCGCGCGCCGCGGGCTTCGGCGCCGCCGTGATCGAGGACGCGTGCCGCGCGATCGATCTCGACGGCTCGCTCGC
GCGCGCATGGGCGTCGCTCGCGGCGGCGGGCGTCGCGCGCGTGCGGTCGGCCGACGCCCATCCCGGCGTCGATCCGTGA

Upstream 100 bases:

>100_bases
ATGCGTGGCAGATCGCGCCTTCGATCGTCGAGCTCGCGAAAAGCGGGCGCGGGTTCAACGCGTGACGCGCGGCCGCGTCG
CCCATTGACAGAGCATCACG

Downstream 100 bases:

>100_bases
TCATCGCCGTCGTTCATTTCATCGCATATCGATCCACAGAATTCAGAATCAGGAGACTCGGATGACCGAAGCCGTAATCG
TATCGACCGCCCGCACGGGG

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEIVPVINRLAARFDHVVLTQDWHPRDHVSFAANHPGRAPFSTVELPYGEQV
LWPVHCVQGTQGAALHGDLDIPHARLVIRKGHDASVDSYSAFVEADRTTPTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFGAAVIEDACRAIDLDGSLARAWASLAAAGVARVRSADAHPGVDP

Sequences:

>Translated_212_residues
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEIVPVINRLAARFDHVVLTQDWHPRDHVSFAANHPGRAPFSTVELPYGEQV
LWPVHCVQGTQGAALHGDLDIPHARLVIRKGHDASVDSYSAFVEADRTTPTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFGAAVIEDACRAIDLDGSLARAWASLAAAGVARVRSADAHPGVDP
>Mature_212_residues
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEIVPVINRLAARFDHVVLTQDWHPRDHVSFAANHPGRAPFSTVELPYGEQV
LWPVHCVQGTQGAALHGDLDIPHARLVIRKGHDASVDSYSAFVEADRTTPTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFGAAVIEDACRAIDLDGSLARAWASLAAAGVARVRSADAHPGVDP

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=203, Percent_Identity=42.3645320197044, Blast_Score=147, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI193206735, Length=223, Percent_Identity=33.6322869955157, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543130, Length=223, Percent_Identity=33.1838565022422, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71999339, Length=197, Percent_Identity=35.5329949238579, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=192, Percent_Identity=34.375, Blast_Score=109, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648247, Length=200, Percent_Identity=38.5, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22607; Mature: 22607

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEIVPVINRLAARFDHVVLTQDWHPRDHVSFA
CCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCCCCCCCEEE
ANHPGRAPFSTVELPYGEQVLWPVHCVQGTQGAALHGDLDIPHARLVIRKGHDASVDSYS
CCCCCCCCCCEEECCCCCEEEEEEEEECCCCCCEEECCCCCCCEEEEEECCCCCCCCHHH
AFVEADRTTPTGLAGYLRELGAKRVWCCGLATDYCVAWSALDARAAGFGAAVIEDACRAI
HEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
DLDGSLARAWASLAAAGVARVRSADAHPGVDP
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEIVPVINRLAARFDHVVLTQDWHPRDHVSFA
CCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCCCCCCCEEE
ANHPGRAPFSTVELPYGEQVLWPVHCVQGTQGAALHGDLDIPHARLVIRKGHDASVDSYS
CCCCCCCCCCEEECCCCCEEEEEEEEECCCCCCEEECCCCCCCEEEEEECCCCCCCCHHH
AFVEADRTTPTGLAGYLRELGAKRVWCCGLATDYCVAWSALDARAAGFGAAVIEDACRAI
HEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
DLDGSLARAWASLAAAGVARVRSADAHPGVDP
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]