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The map label for this gene is ispH [H]

Identifier: 83720986

GI number: 83720986

Start: 900307

End: 901287

Strand: Direct

Name: ispH [H]

Synonym: BTH_I0783

Alternate gene names: 83720986

Gene position: 900307-901287 (Clockwise)

Preceding gene: 83718454

Following gene: 83720418

Centisome position: 23.64

GC content: 67.18

Gene sequence:

>981_bases
ATGAGCTCCACCGATACGTTGTCCGGCCAGGTCGCCGCGGCCGACGCCGAGATTCTGCTCGCCCAGCCGCGCGGTTTCTG
CGCGGGCGTCGATCGCGCGATCGAGATCGTCGAGCGTGCGATCGCGATGCACGGCGCGCCGATCTACGTGCGCCACGAGA
TCGTGCACAACAAGTACGTCGTCGAGGACCTGAAAAAGAAGGGCGCGATTTTCGTCGAGGAACTCGAGGAAGTGCCGTCC
GGCAACACGGTGATCTTCAGCGCGCACGGCGTATCGAAGGCGGTGCGCGACGAGGCGGCCGTGCGCGGGCTGCGCATTTA
CGACGCGACGTGCCCGCTCGTCACGAAGGTGCACGTCGAGGTCGCGAAGATGCGCCAGGAAGGCGTCGACATCGTGATGA
TCGGCCACAAGGGCCATCCGGAGGTTGAAGGCACGATGGGGCAGGTCGAGCGCGGCATGCATCTCGTCGAGAGCGTCGAG
GACGTGCTCAAGCTCGAACTGCCCGATCCGGAGCGCGTCGCGCTCGTCACGCAGACGACGCTGTCCGTCGACGACGCGGC
CGAGATCATCGGCGCGCTCAAGGCGAAATTTCCCGCGATTCGCGAGCCGAAGAAGCAGGACATCTGCTACGCGACGCAGA
ACCGCCAGGACGCGGTGAAGTTCATGGCGCCGCAATGCGACGTCGTGATCGTCGTCGGCAGCCCGAACAGCTCGAATTCG
AGCCGTTTGCGCGAGGTCGCCGAGAAGCGCGGCGTCGCCGCCTACATGGTCGACGCGCCCGAGCAGATCGACCCGGCCTG
GGTGGCCGGCAAGCGCCGGATCGGCGTGACCGCGGGCGCGTCGGCGCCCGAAGTGCTCGCGCAGGCCGTGATCGCGCGTT
TGCGCGAGCTCGGCGTGACCAACGTGCGGGCGCTCGAGGGCATCGAGGAGAACGTGTCGTTCCCGTTGCCGCGCGGCCTG
AATCTGTCTTCCGCCGCCTGA

Upstream 100 bases:

>100_bases
TCAAGGAGATCGGCGAGACCTCCGCGCTTTTCGATTTCAACCATCCGCTCGCGGGCCAGGCGCTCACGTTCGAAGTGAAA
ATCATCGGGATTCTGTAATC

Downstream 100 bases:

>100_bases
TCGCCGGTTTCGTTTCCGTCGCAATGCAAAAGCGCGCCCCGGGCGCGCTTTTCTTTTGCGCGCGATATGTGCGCGATTTT
CCGGAGAGCAACCATCCGGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSSTDTLSGQVAAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYVVEDLKKKGAIFVEELEEVPS
GNTVIFSAHGVSKAVRDEAAVRGLRIYDATCPLVTKVHVEVAKMRQEGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVE
DVLKLELPDPERVALVTQTTLSVDDAAEIIGALKAKFPAIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNS
SRLREVAEKRGVAAYMVDAPEQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVTNVRALEGIEENVSFPLPRGL
NLSSAA

Sequences:

>Translated_326_residues
MSSTDTLSGQVAAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYVVEDLKKKGAIFVEELEEVPS
GNTVIFSAHGVSKAVRDEAAVRGLRIYDATCPLVTKVHVEVAKMRQEGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVE
DVLKLELPDPERVALVTQTTLSVDDAAEIIGALKAKFPAIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNS
SRLREVAEKRGVAAYMVDAPEQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVTNVRALEGIEENVSFPLPRGL
NLSSAA
>Mature_325_residues
SSTDTLSGQVAAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYVVEDLKKKGAIFVEELEEVPSG
NTVIFSAHGVSKAVRDEAAVRGLRIYDATCPLVTKVHVEVAKMRQEGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVED
VLKLELPDPERVALVTQTTLSVDDAAEIIGALKAKFPAIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNSS
RLREVAEKRGVAAYMVDAPEQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVTNVRALEGIEENVSFPLPRGLN
LSSAA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=57.9288025889968, Blast_Score=373, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35092; Mature: 34961

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTDTLSGQVAAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYV
CCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
VEDLKKKGAIFVEELEEVPSGNTVIFSAHGVSKAVRDEAAVRGLRIYDATCPLVTKVHVE
HHHHHHCCCEEHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHH
VAKMRQEGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVEDVLKLELPDPERVALVTQTT
HHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEE
LSVDDAAEIIGALKAKFPAIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEEECCCCCCH
SRLREVAEKRGVAAYMVDAPEQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVT
HHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
NVRALEGIEENVSFPLPRGLNLSSAA
HHHHHHCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSTDTLSGQVAAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYV
CCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
VEDLKKKGAIFVEELEEVPSGNTVIFSAHGVSKAVRDEAAVRGLRIYDATCPLVTKVHVE
HHHHHHCCCEEHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHH
VAKMRQEGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVEDVLKLELPDPERVALVTQTT
HHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEE
LSVDDAAEIIGALKAKFPAIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEEECCCCCCH
SRLREVAEKRGVAAYMVDAPEQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVT
HHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
NVRALEGIEENVSFPLPRGLNLSSAA
HHHHHHCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA