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The map label for this gene is tkrA [H]

Identifier: 83720963

GI number: 83720963

Start: 2589948

End: 2590925

Strand: Direct

Name: tkrA [H]

Synonym: BTH_I2298

Alternate gene names: 83720963

Gene position: 2589948-2590925 (Clockwise)

Preceding gene: 83719833

Following gene: 83718412

Centisome position: 67.99

GC content: 70.86

Gene sequence:

>978_bases
ATGAAGCATCGCATCGTCGTCTACAAGCCGCTTCCCGGCGACGTGCTCGCGACCCTGCGCGAGCGCGCCGACGTCGTGCT
CGTCGAGGGCGCCGACGCGCTCGCGCGCGCGCTGCCCGACGCCGACGGCGCGCTCGGCGCGAGCCTGAAGATCACGCCCG
AGCTGCTCGATCTCGCGCCTCGGCTGCGCGCGTGGTCGACGATCTCGGTGGGCTTCGACAACTTCGACGTCGCCGATCTG
ACGCGCCGCGGGATCGTGCTCGCGCACACGCCGGACGTGCTGACCGAGGCGACCGCCGACACCGTGTTCGCGCTGATTCT
CGCGAGCGCGCGGCGCGTCGTCGAGCTCGCCGAGTTCGTGAAGGCGGGGCAGTGGCGGCAGAGTATCGGCGAGTCGCTCT
ACGGCACCGACGTGAACGGCAAGACGCTCGGCATCGTCGGGCTCGGGCGCATCGGCGCCGCGGTCGCGCGCCGCGCGGCG
CTCGGCTTCCGGATGCCGGTGCTCTACACGAACCGCAGCGCGAACCTGGAGGCGCAGGCGCAGTTCGGCGCGCGCCGCGT
CGAGCTCGACGAATTGCTCGCCGAGTCCGACTTCGTGTGCGTGCAAGTGCCGCTTTCGCCGCAAACACGGCATCTGATCG
GCGCGCGCGAGCTCGGGAAGATGAAGCGCGGCGCGATCCTCGTGAACGCGTCGCGCGGGCCCGTCGTCGACGAGTCGGCG
CTGATCGACGCACTGCGCGCGGGAACGATTCGTGCGGCGGGTCTCGACGTGTTCGAGCGCGAACCGCTCGCCGTCGATTC
GCCGTTGCTGTCGATGAACAACGTCGTCGCGCTGCCGCATATCGGCTCGGCGACGCACGAAACGCGCCGTGCGATGGCGC
GCTGCGCGGCCGAAAACGTGATCGCGGCGCTCGACGGCACGCTTGCGTGCAATATCGTCAATCGCGACGTGCTGCGGCGC
GCGCCGTCGGCGCCTTGA

Upstream 100 bases:

>100_bases
ATGAGCGCCGCGCTCGTCGCGGCCGTCGTGCTCACGCTGTCGGTGAAGCAGGCGGGCGCGACGCCGACGCTCGCCCATCC
GTTGCAAGGAAAGTGAATAC

Downstream 100 bases:

>100_bases
GCGCCGAGCCGATCGCGCGCGGCGGGTTTGCCGCGCGCGCGGGCGGCGCATCGGGGCGGCGGCGCGCTCGCGTGGCGGGC
TTTCGCGCGTGTTGCTCGCG

Product: 2-ketogluconate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKHRIVVYKPLPGDVLATLRERADVVLVEGADALARALPDADGALGASLKITPELLDLAPRLRAWSTISVGFDNFDVADL
TRRGIVLAHTPDVLTEATADTVFALILASARRVVELAEFVKAGQWRQSIGESLYGTDVNGKTLGIVGLGRIGAAVARRAA
LGFRMPVLYTNRSANLEAQAQFGARRVELDELLAESDFVCVQVPLSPQTRHLIGARELGKMKRGAILVNASRGPVVDESA
LIDALRAGTIRAAGLDVFEREPLAVDSPLLSMNNVVALPHIGSATHETRRAMARCAAENVIAALDGTLACNIVNRDVLRR
APSAP

Sequences:

>Translated_325_residues
MKHRIVVYKPLPGDVLATLRERADVVLVEGADALARALPDADGALGASLKITPELLDLAPRLRAWSTISVGFDNFDVADL
TRRGIVLAHTPDVLTEATADTVFALILASARRVVELAEFVKAGQWRQSIGESLYGTDVNGKTLGIVGLGRIGAAVARRAA
LGFRMPVLYTNRSANLEAQAQFGARRVELDELLAESDFVCVQVPLSPQTRHLIGARELGKMKRGAILVNASRGPVVDESA
LIDALRAGTIRAAGLDVFEREPLAVDSPLLSMNNVVALPHIGSATHETRRAMARCAAENVIAALDGTLACNIVNRDVLRR
APSAP
>Mature_325_residues
MKHRIVVYKPLPGDVLATLRERADVVLVEGADALARALPDADGALGASLKITPELLDLAPRLRAWSTISVGFDNFDVADL
TRRGIVLAHTPDVLTEATADTVFALILASARRVVELAEFVKAGQWRQSIGESLYGTDVNGKTLGIVGLGRIGAAVARRAA
LGFRMPVLYTNRSANLEAQAQFGARRVELDELLAESDFVCVQVPLSPQTRHLIGARELGKMKRGAILVNASRGPVVDESA
LIDALRAGTIRAAGLDVFEREPLAVDSPLLSMNNVVALPHIGSATHETRRAMARCAAENVIAALDGTLACNIVNRDVLRR
APSAP

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=278, Percent_Identity=43.1654676258993, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI23308577, Length=296, Percent_Identity=33.7837837837838, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4557497, Length=297, Percent_Identity=35.3535353535354, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI61743967, Length=297, Percent_Identity=35.3535353535354, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI145580578, Length=294, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557499, Length=294, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI145580575, Length=281, Percent_Identity=33.4519572953737, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI87082289, Length=322, Percent_Identity=57.4534161490683, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789279, Length=271, Percent_Identity=33.210332103321, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787645, Length=192, Percent_Identity=31.25, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87081824, Length=175, Percent_Identity=34.8571428571429, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=294, Percent_Identity=32.9931972789116, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=286, Percent_Identity=29.3706293706294, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=232, Percent_Identity=42.6724137931034, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320925, Length=257, Percent_Identity=33.852140077821, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=277, Percent_Identity=32.129963898917, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321253, Length=183, Percent_Identity=32.7868852459016, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325144, Length=194, Percent_Identity=30.9278350515464, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324964, Length=242, Percent_Identity=28.9256198347107, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324980, Length=163, Percent_Identity=31.2883435582822, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45552429, Length=310, Percent_Identity=39.6774193548387, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI45551003, Length=310, Percent_Identity=39.6774193548387, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24585514, Length=310, Percent_Identity=39.6774193548387, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574282, Length=310, Percent_Identity=39.6774193548387, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28571528, Length=281, Percent_Identity=44.1281138790036, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574284, Length=310, Percent_Identity=39.6774193548387, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574286, Length=256, Percent_Identity=41.796875, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24585516, Length=311, Percent_Identity=33.7620578778135, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI19921140, Length=282, Percent_Identity=36.5248226950355, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24646446, Length=298, Percent_Identity=35.2348993288591, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646448, Length=298, Percent_Identity=35.2348993288591, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646452, Length=298, Percent_Identity=35.2348993288591, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646450, Length=298, Percent_Identity=35.2348993288591, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI62472511, Length=316, Percent_Identity=34.8101265822785, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34667; Mature: 34667

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHRIVVYKPLPGDVLATLRERADVVLVEGADALARALPDADGALGASLKITPELLDLAP
CCCCEEEEECCCHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCCCCCEEEECHHHHHHHH
RLRAWSTISVGFDNFDVADLTRRGIVLAHTPDVLTEATADTVFALILASARRVVELAEFV
HHHCCEEEEECCCCCCHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAGQWRQSIGESLYGTDVNGKTLGIVGLGRIGAAVARRAALGFRMPVLYTNRSANLEAQA
HHCHHHHHHCCHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
QFGARRVELDELLAESDFVCVQVPLSPQTRHLIGARELGKMKRGAILVNASRGPVVDESA
HHCCHHCCHHHHHCCCCEEEEEECCCCCHHHHHCHHHHHHHCCCEEEEECCCCCCCCHHH
LIDALRAGTIRAAGLDVFEREPLAVDSPLLSMNNVVALPHIGSATHETRRAMARCAAENV
HHHHHHCCCEEECCCCHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
IAALDGTLACNIVNRDVLRRAPSAP
HHHHCCEEEEEECCHHHHHCCCCCC
>Mature Secondary Structure
MKHRIVVYKPLPGDVLATLRERADVVLVEGADALARALPDADGALGASLKITPELLDLAP
CCCCEEEEECCCHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCCCCCEEEECHHHHHHHH
RLRAWSTISVGFDNFDVADLTRRGIVLAHTPDVLTEATADTVFALILASARRVVELAEFV
HHHCCEEEEECCCCCCHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAGQWRQSIGESLYGTDVNGKTLGIVGLGRIGAAVARRAALGFRMPVLYTNRSANLEAQA
HHCHHHHHHCCHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
QFGARRVELDELLAESDFVCVQVPLSPQTRHLIGARELGKMKRGAILVNASRGPVVDESA
HHCCHHCCHHHHHCCCCEEEEEECCCCCHHHHHCHHHHHHHCCCEEEEECCCCCCCCHHH
LIDALRAGTIRAAGLDVFEREPLAVDSPLLSMNNVVALPHIGSATHETRRAMARCAAENV
HHHHHHCCCEEECCCCHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
IAALDGTLACNIVNRDVLRRAPSAP
HHHHCCEEEEEECCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA