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The map label for this gene is queF [H]

Identifier: 83720941

GI number: 83720941

Start: 623202

End: 624026

Strand: Reverse

Name: queF [H]

Synonym: BTH_I0548

Alternate gene names: 83720941

Gene position: 624026-623202 (Counterclockwise)

Preceding gene: 83720819

Following gene: 83720707

Centisome position: 16.38

GC content: 65.94

Gene sequence:

>825_bases
ATGAACCCCGAACACTCCCCGCTCGGCAAGACGACCGTCTACGCGAACCAGTACGACGCGTCGCTGCTGTTTCCGATTCC
GCGCGCGGGCGCGCGCGAGCAGATCGGCATCGGCGCGCCGCTGCCGTTCTTCGGCACCGACATCTGGAACGCGTACGAAC
TGTCGTGGCTCAATGCGCGCGGCAAGCCGCAAGTGGCGATCGCGACGTTCTACGTGCCCGCCGAATCGCCCAACATCGTC
GAATCAAAATCGTTCAAGCTGTATCTCGGCTCGTTCGCGCAGACCGCGTTCGAATCGATCGACGCGGTGCGCGACGCGCT
CAAGCGCGACGTGTCGGCCGCGTGCGGCGCGAGCGTGACCGTACGGCTCGCGACGCCCGCCGAATTCCGCAAGCTGCAGA
TGGACGAGCTCGACGGGCTGTCGCTCGATCGCCTCGATCTCGACGCCGACGTCTACGAGACCGATCCGTCGTTCCTCACC
GCATCGCACGACGAGGCGCCCGTCGAGGAGACGCTCGTCACCGATCTGCTGAAGTCAAACTGCCCGGTGACGGGCCAGCC
CGACTGGGGCAGCGTGCAGATCCACTACGTCGGCGCGCCGATCGATCACGCGGGCCTGCTGCGCTACATCATCTCGTTTC
GCAACCACACGGGCTTTCACGAGCAGTGCGTCGAGCGGATCTTCATCGACATCCTGCGCGCGTGCAAGCCGGTGAAGCTC
GCGGTCTACGCACGCTACACGCGCCGCGGCGGGCTCGACATCAATCCGTTCCGCACGAACTACAACCAGCCGATGCCGGA
CAACGCGCGCACCGCGCGGCAGTGA

Upstream 100 bases:

>100_bases
TTCGACCGCTTTCTCGCGGCGCTCGGCTATCCGTTCTGGGAAGAAAGCGTCAATCCGGCGTATCGTCTCTTCCTGTCGTA
ATCCGTCCATTCCATCCGAT

Downstream 100 bases:

>100_bases
CGCAGGCGGCGCGCGCCGTTGCCGGTCGTCACCTCGCGCCCGCGCGCCGACTGCCGGTTGCCGCGCAACGCGGAAATAGC
GGCGAGCGCCGCGCGATTCG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNPEHSPLGKTTVYANQYDASLLFPIPRAGAREQIGIGAPLPFFGTDIWNAYELSWLNARGKPQVAIATFYVPAESPNIV
ESKSFKLYLGSFAQTAFESIDAVRDALKRDVSAACGASVTVRLATPAEFRKLQMDELDGLSLDRLDLDADVYETDPSFLT
ASHDEAPVEETLVTDLLKSNCPVTGQPDWGSVQIHYVGAPIDHAGLLRYIISFRNHTGFHEQCVERIFIDILRACKPVKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ

Sequences:

>Translated_274_residues
MNPEHSPLGKTTVYANQYDASLLFPIPRAGAREQIGIGAPLPFFGTDIWNAYELSWLNARGKPQVAIATFYVPAESPNIV
ESKSFKLYLGSFAQTAFESIDAVRDALKRDVSAACGASVTVRLATPAEFRKLQMDELDGLSLDRLDLDADVYETDPSFLT
ASHDEAPVEETLVTDLLKSNCPVTGQPDWGSVQIHYVGAPIDHAGLLRYIISFRNHTGFHEQCVERIFIDILRACKPVKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
>Mature_274_residues
MNPEHSPLGKTTVYANQYDASLLFPIPRAGAREQIGIGAPLPFFGTDIWNAYELSWLNARGKPQVAIATFYVPAESPNIV
ESKSFKLYLGSFAQTAFESIDAVRDALKRDVSAACGASVTVRLATPAEFRKLQMDELDGLSLDRLDLDADVYETDPSFLT
ASHDEAPVEETLVTDLLKSNCPVTGQPDWGSVQIHYVGAPIDHAGLLRYIISFRNHTGFHEQCVERIFIDILRACKPVKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=269, Percent_Identity=53.1598513011152, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 30452; Mature: 30452

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEHSPLGKTTVYANQYDASLLFPIPRAGAREQIGIGAPLPFFGTDIWNAYELSWLNAR
CCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
GKPQVAIATFYVPAESPNIVESKSFKLYLGSFAQTAFESIDAVRDALKRDVSAACGASVT
CCCCEEEEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VRLATPAEFRKLQMDELDGLSLDRLDLDADVYETDPSFLTASHDEAPVEETLVTDLLKSN
EEEECCHHHHHHHHHHCCCCCCEEEECCCCEEECCCCEEECCCCCCCHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGAPIDHAGLLRYIISFRNHTGFHEQCVERIFIDILRACKPVKL
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
EEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPEHSPLGKTTVYANQYDASLLFPIPRAGAREQIGIGAPLPFFGTDIWNAYELSWLNAR
CCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
GKPQVAIATFYVPAESPNIVESKSFKLYLGSFAQTAFESIDAVRDALKRDVSAACGASVT
CCCCEEEEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VRLATPAEFRKLQMDELDGLSLDRLDLDADVYETDPSFLTASHDEAPVEETLVTDLLKSN
EEEECCHHHHHHHHHHCCCCCCEEEECCCCEEECCCCEEECCCCCCCHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGAPIDHAGLLRYIISFRNHTGFHEQCVERIFIDILRACKPVKL
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
EEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA