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The map label for this gene is potI [H]

Identifier: 83720931

GI number: 83720931

Start: 2567140

End: 2567958

Strand: Direct

Name: potI [H]

Synonym: BTH_I2279

Alternate gene names: 83720931

Gene position: 2567140-2567958 (Clockwise)

Preceding gene: 83720205

Following gene: 83718770

Centisome position: 67.39

GC content: 63.37

Gene sequence:

>819_bases
ATGAAGCCGAATCGCTACTTGCAGGTGCTGATGCTCGCGATCGGCTTCGGTTTCCTCTATATCCCGATCGCGAGCCTCGT
CGTCTACTCGTTCAACGAATCGAAGCTCGTCACCGTCTGGTCCGGCTTCTCGACGCGCTGGTACACGGCGCTCCTCGAGG
ACGACGAACTGATAACGGCCGCGTGGCTGTCGCTGAAAATCGCGGTGATGACCGCATTCGCGTCGGTGTTCATCGGCACG
TGGGCAGGCTTCGTGCTCGCGCGGATGGGCCGCTTTCGCGGCTTCGCGCTCTACAGCGGGATGATCAACGCGCCGCTCGT
GATTCCCGAGGTGATCCAGGGCATCTCGCTGCTGCTGCTGTTCATCGAGCTCGGCAAGTGGTTCGGGTGGCCCGCCGGCC
GCGGGATGTTCACGATCTGGCTCGGCCACGTGATGCTGTGCATCTCGTACGTGGCGATCATCGTGCAGTCGCGCGTGCGC
GAGCTGAACCCGTCGCTCGAGGAGGCCGCGCTCGATCTCGGCGCGACGCCGTTCAAGGTGTTCTTCTCGATCACGCTGCC
GCTGATCTCGCAGGCGCTCGTGTCCGGCTGGTTGTTGTCGTTCACGCTGTCGATCGACGATCTGGTGTTGTCGGCGTTCC
TGTCCGGGCCCGGCTCGACCACATTGCCGCTCGTCGTGTTCTCGCGCGTGCGCCTCGGCCTCAATCCGGAAATGAACGCG
CTCGCGACGCTGTTCATCGTCGCGGTGACGATCGGCGTCGTGGTGGCGAACTACGTGATGCAGCGTCAGGAAAGGAAGAG
GATGGCCGAAGCCGTCTGA

Upstream 100 bases:

>100_bases
CGATGGCGTCGGCCGTGACCTGCGCGATGGTGCTGCTGCTGCTCGTGCCGATGGCGATGTTCCAGCACTTCCAGGCGAAG
GAGCAGGAGGGCCGCCGCAA

Downstream 100 bases:

>100_bases
CGCGGCTGCGCGGGCGGCGGCCCGATGAAGGGGCTGCCGCGCGCGGCGCGTTGCCGTCGCTCGCGTGAGCGAAACCCGAG
ACAGCGCCCGTGCGGCCGAA

Product: putrescine ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNESKLVTVWSGFSTRWYTALLEDDELITAAWLSLKIAVMTAFASVFIGT
WAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPFKVFFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVVVANYVMQRQERKRMAEAV

Sequences:

>Translated_272_residues
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNESKLVTVWSGFSTRWYTALLEDDELITAAWLSLKIAVMTAFASVFIGT
WAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPFKVFFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVVVANYVMQRQERKRMAEAV
>Mature_272_residues
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNESKLVTVWSGFSTRWYTALLEDDELITAAWLSLKIAVMTAFASVFIGT
WAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPFKVFFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVVVANYVMQRQERKRMAEAV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=263, Percent_Identity=58.93536121673, Blast_Score=290, Evalue=7e-80,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=39.3305439330544, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=29.296875, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788764, Length=215, Percent_Identity=30.6976744186047, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30058; Mature: 30058

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNESKLVTVWSGFSTRWYTALLEDDELITA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHH
AWLSLKIAVMTAFASVFIGTWAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVRELNPSLEEAALDLGATPFKV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIVAVTIGVVVANYVMQRQERKRMAEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNESKLVTVWSGFSTRWYTALLEDDELITA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHH
AWLSLKIAVMTAFASVFIGTWAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVRELNPSLEEAALDLGATPFKV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIVAVTIGVVVANYVMQRQERKRMAEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]