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The map label for this gene is kgtP [H]

Identifier: 83720905

GI number: 83720905

Start: 2818779

End: 2820083

Strand: Reverse

Name: kgtP [H]

Synonym: BTH_I2466

Alternate gene names: 83720905

Gene position: 2820083-2818779 (Counterclockwise)

Preceding gene: 83720175

Following gene: 83718831

Centisome position: 74.03

GC content: 66.44

Gene sequence:

>1305_bases
ATGAATGACCAGACCGCCGTTGCCGCCGTCGCGCGGCAAGACGTCCGGCGTCGCGTGCTCGCGATCGTCGGCGCTTCGTC
GGGCAACCTCGTCGAGTGGTTCGACTTCTATATCTATTCGTTCTGCGCGCTGTACTTCGCGCCGGCGTTCTTCCCGAGCG
GCAACACGACCACGCAGCTTCTCAACACCGCGGGCGTGTTCGCCGCGGGCTTCCTGATGCGTCCGATCGGCGGCTGGCTG
TTCGGCCGGATCGCCGACAAGCACGGCCGCCGCGCCGCGATGATGATCTCGGTGCTGATGATGTGCGGCGGCTCGCTCGT
GATCGCGGTGCTGCCGACGTACGCGCAGATCGGCGCGTTCGCGCCGTTGCTGCTGCTCGTCGCGCGGCTGTTCCAGGGCC
TGTCGGTGGGCGGCGAGTACGGCACGAGCGCGACGTACATGAGCGAGGTCGCGCTCAAGGGCCGCCGCGGCTTCTTCGCA
TCGTTCCAGTACGTGACGCTGATCGGCGGCCAGCTCTGCGCGCTCCTCGTGCTCGTGATCCTGCAGCAGACGCTTTCGAC
CGCCGAGCTGAAGGCGTGGGGCTGGCGGATTCCGTTCGTCGTCGGCGCGGCGGCCGCGCTGATCTCGCTGTATCTGCGCA
AGTCGCTCGACGAGACGTCGACGAGCGAATCGCGCAAGGCGAAGGACGCCGGCACGATCCGCGGCGTGTGGCAGCACAAG
GGCGCGTTCCTGACCGTCATCGGCTTCACGGCCGGCGGCTCGCTGATCTTCTACACGTTCACCACGTACATGCAGAAGTA
CCTCGTCAACACGGCGGGCATGCACGCGAAGACCGCGAGCAACGTGATGACGGCCGCGCTCTTCGTCTACATGCTGATGC
AGCCCGTGTTCGGCGCGCTGTCGGACAAGATCGGCCGCCGGATGTCGATGATCCTGTTCGGCACGGGCGCCGTGATCGGC
ACGGTGCCGCTGATGAATGCGCTCGGCGGCGTGACGAGCCCGTTCGCGGCGTTCGGCCTCATCGTCGTCGCGCTCGCGAT
CGTCAGCTTCTACACGTCGATCAGCGGCCTCATCAAGGCCGAGATGTTCCCGCCCGAGGTGCGCGCGATGGGCGTCGGCC
TGTCGTACGCGGTCGCGAATGCGATCTTCGGGGGCTCGGCTGAATACGTCGCGCTGTGGTTCAAGTCGGTCGGCAGCGAA
TCGAGCTTCTACTGGTATGTGACCGTGCTGTGCGCGATCTCGCTTTTCGTGTCGTGGCGGATGCGCGATCCGAGCAAGGA
CGGCTACCTGCGCAACGAGCCCTGA

Upstream 100 bases:

>100_bases
ACGCCGAGGTGGAACCGGTGTCACCGCGTGCGTTGAACAAGAAGGCGGCAGTTGTCGTCTCCGCCAGGCCATCCAGAGAA
CTTCAAGGAACAGACCATCC

Downstream 100 bases:

>100_bases
GTGCGGCCCGGCCGCGCGGCGGGCGGCGTCCGGTTCGGCGCCCGCCGCGCGACGCGCCTTCTGTGGCGGCGCGGCGGGGA
ATCCGTGTCGCGCATGCACA

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNDQTAVAAVARQDVRRRVLAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADKHGRRAAMMISVLMMCGGSLVIAVLPTYAQIGAFAPLLLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSTAELKAWGWRIPFVVGAAAALISLYLRKSLDETSTSESRKAKDAGTIRGVWQHK
GAFLTVIGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPVFGALSDKIGRRMSMILFGTGAVIG
TVPLMNALGGVTSPFAAFGLIVVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSVGSE
SSFYWYVTVLCAISLFVSWRMRDPSKDGYLRNEP

Sequences:

>Translated_434_residues
MNDQTAVAAVARQDVRRRVLAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADKHGRRAAMMISVLMMCGGSLVIAVLPTYAQIGAFAPLLLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSTAELKAWGWRIPFVVGAAAALISLYLRKSLDETSTSESRKAKDAGTIRGVWQHK
GAFLTVIGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPVFGALSDKIGRRMSMILFGTGAVIG
TVPLMNALGGVTSPFAAFGLIVVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSVGSE
SSFYWYVTVLCAISLFVSWRMRDPSKDGYLRNEP
>Mature_434_residues
MNDQTAVAAVARQDVRRRVLAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADKHGRRAAMMISVLMMCGGSLVIAVLPTYAQIGAFAPLLLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSTAELKAWGWRIPFVVGAAAALISLYLRKSLDETSTSESRKAKDAGTIRGVWQHK
GAFLTVIGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPVFGALSDKIGRRMSMILFGTGAVIG
TVPLMNALGGVTSPFAAFGLIVVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSVGSE
SSFYWYVTVLCAISLFVSWRMRDPSKDGYLRNEP

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=422, Percent_Identity=70.1421800947867, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1790550, Length=416, Percent_Identity=28.3653846153846, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=29.9043062200957, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1789941, Length=392, Percent_Identity=33.1632653061224, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI87082231, Length=313, Percent_Identity=26.1980830670927, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082404, Length=313, Percent_Identity=26.8370607028754, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46802; Mature: 46802

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]