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The map label for this gene is mexA [H]

Identifier: 83720836

GI number: 83720836

Start: 779764

End: 781026

Strand: Direct

Name: mexA [H]

Synonym: BTH_I0680

Alternate gene names: 83720836

Gene position: 779764-781026 (Clockwise)

Preceding gene: 83720183

Following gene: 83721001

Centisome position: 20.47

GC content: 69.68

Gene sequence:

>1263_bases
ATGCGCGTCGAACGGGTTCCATACCGCTTAATCACTGTCGCGACGGCTGCCGTTTTTCTGGCCGCGTGCGGAAAAAAAGA
ATCGGCTCCGCCCCCTCAAACGCCCGAAGTCGGCGTCGTCACCGTCCAGCCGCAAGCCGTGCCGGTCGTCTCCGAATTGC
CGGGCCGCACGAGCGCGTATCTGGTCGCGCAGGTGCGCGCGCGGGTGGACGGCATCGTGTTGCGGCGTGAGTTCACGGAA
GGCAGCGACGTCAAGGCCGGCCAGCGTCTGTACAAGATCGATCCCGCCCCGTACATCGCGCAGTTGAACAGCGCGAAGGC
GACGCTCGCGAAGGCGCAGGCGAATCTCGCGACGCAGAACGCGCTCGTCGCGCGCTACAAGGTGCTCGTCGCCGCGAACG
CGGTCAGCAAGCAGGACTACGACAACGCGGTGGCCACGCAAGGGCAGGCCGCCGCCGACGTGGCGGCCGGCAAGGCCGCG
GTCGACACCGCGCAGATCAACCTCGGCTATACGGACGTGGTCTCGCCGATCACGGGCCGCGTCGGCATCTCGCAGGTCAC
GCCGGGCGCGTACGTGCAGGCGAGCCAGGCGACGCTGATGTCGACCGTCCAGCAGCTCGATCCCGTCTACGTCGACCTCA
CGCAGTCGAGCCTCGACGGCCTGAAGCTGCGCCAGGACATCCAGAGCGGGCGCATCAAGACGGAGGGTCCGGGCGCGGCG
AAGGTCACGCTGATCCTCGAGGACGGCAAGGCGTACTCGGAGCCGGGCAAGCTGCAGTTCAGCGACGTCACGGTCGACCA
GACGACGGGCTCGGTCACGATTCGCGCGATCTTCCCCAACAAGCAGCGCGTGCTGCTGCCGGGCATGTTCGTGCGCGCGC
GCATCGAAGAGGGCGTCAACGACAACGCGTTCCTCGTCCCGCAGATCGGCGTCACGCGCGATCCGAAGGGCCAGGCGGTC
GCGATGATCGTCGACGGCAAGGGCAAGGTCGAGCCGCGCGTGCTCACGACGAGCGGCACGCAGGGCCAGAACTGGGTCGT
CGAAGGCGGCCTGCAGGCGGGCGACCGCGTGATCGTGCAGGGCATCGACAAGGTGCGCCCGGGCATGACCGTGAAGACCG
CCGAAGCTCAATTGCCGGCCGCGGCGTCCGGCGCGTCCGGCGCCGCGCCCGCGGGCGGCTCGCCCGCCCAGGCCGCGGCG
GCGAGCGCGGCCGCATCGGGTGCCGCGCCGTCGAGCGCTGCCGCCGCGTCGAGCGCGAAATAA

Upstream 100 bases:

>100_bases
AGTCAAGCAATTCAGTTGTCCGATCCGCGTCGCAAGGCGCGTTCGTGTTGTGTCCCCGAGGCCGGCACTTATTTCATCTA
GTTACAAACGAGGTCGCTCC

Downstream 100 bases:

>100_bases
CAGGGGGCCTGCTTCATGGCAAAGTTTTTTATCGATCGCCCGATCTTCGCGTGGGTGATCGCCATCATCCTGATGCTGGC
CGGCGTCGCGGCGATCTTCA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVVSELPGRTSAYLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAA
VDTAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
KVTLILEDGKAYSEPGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGVNDNAFLVPQIGVTRDPKGQAV
AMIVDGKGKVEPRVLTTSGTQGQNWVVEGGLQAGDRVIVQGIDKVRPGMTVKTAEAQLPAAASGASGAAPAGGSPAQAAA
ASAAASGAAPSSAAAASSAK

Sequences:

>Translated_420_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVVSELPGRTSAYLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAA
VDTAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
KVTLILEDGKAYSEPGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGVNDNAFLVPQIGVTRDPKGQAV
AMIVDGKGKVEPRVLTTSGTQGQNWVVEGGLQAGDRVIVQGIDKVRPGMTVKTAEAQLPAAASGASGAAPAGGSPAQAAA
ASAAASGAAPSSAAAASSAK
>Mature_420_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVVSELPGRTSAYLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAA
VDTAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
KVTLILEDGKAYSEPGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGVNDNAFLVPQIGVTRDPKGQAV
AMIVDGKGKVEPRVLTTSGTQGQNWVVEGGLQAGDRVIVQGIDKVRPGMTVKTAEAQLPAAASGASGAAPAGGSPAQAAA
ASAAASGAAPSSAAAASSAK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=385, Percent_Identity=52.987012987013, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789665, Length=361, Percent_Identity=50.6925207756233, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=50.2747252747253, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI87082036, Length=361, Percent_Identity=31.3019390581717, Blast_Score=151, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43218; Mature: 43218

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVVSELPGRTSAY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCHHH
LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAAVDTAQINLGYTDVVSPITGR
HHHHHEEEEEEECHHCCCCCCCHHHCCCCHHHHHHCCCCEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
CCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEECCCCCE
KVTLILEDGKAYSEPGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEECCHHHHHHHHCCCC
DNAFLVPQIGVTRDPKGQAVAMIVDGKGKVEPRVLTTSGTQGQNWVVEGGLQAGDRVIVQ
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEE
GIDKVRPGMTVKTAEAQLPAAASGASGAAPAGGSPAQAAAASAAASGAAPSSAAAASSAK
CHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVVSELPGRTSAY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCHHH
LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAAVDTAQINLGYTDVVSPITGR
HHHHHEEEEEEECHHCCCCCCCHHHCCCCHHHHHHCCCCEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
CCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEECCCCCE
KVTLILEDGKAYSEPGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEECCHHHHHHHHCCCC
DNAFLVPQIGVTRDPKGQAVAMIVDGKGKVEPRVLTTSGTQGQNWVVEGGLQAGDRVIVQ
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEE
GIDKVRPGMTVKTAEAQLPAAASGASGAAPAGGSPAQAAAASAAASGAAPSSAAAASSAK
CHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]