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The map label for this gene is ada [H]

Identifier: 83720827

GI number: 83720827

Start: 89582

End: 90676

Strand: Direct

Name: ada [H]

Synonym: BTH_I0085

Alternate gene names: 83720827

Gene position: 89582-90676 (Clockwise)

Preceding gene: 83718395

Following gene: 83721464

Centisome position: 2.35

GC content: 70.68

Gene sequence:

>1095_bases
ATGAAGCAGCCAGCCTATCCGACCGACGATGCACGCTGGGCCGCGCTCGTCGCGCGCGACGCCGACGCCGACGGTGCGTT
CTGCTACGCGGTCAAGACGACGGGCGTGTTCTGCCGGCCGTCGTGCGCGTCGCGGCTGCCGAGGCGCGAGAACGTGTCGT
TCTTCGCCGACACGGACGCCGCGCGCGCGGCCGGCTATCGTCCGTGCAAGCGCTGTCGGCCCGAGGGGTTGCCGCGTGAG
CTCGAGATCGTCAATCGTGCGTGCGTGGTGCTTGACGCGCATCGTCAGGACCGCTTCACGCTCGCGCAATTGAGCGACGC
GGTTCACGTGAGCCCGTTCCATCTTCAACGGCTGTTCAAGCGCGTCGTCGGCGTGTCGCCGCGGCAATACCAGGCCGCGC
AGCGCGGCGCCGCGTTGCGCGACGCGTTGCAAGGCGGCGCCGCGGTCACGCGCGCGGCCGTCGACGCGGGCTTCAATTCG
CCGTCGCGGCTCTACGAATCGGTTCCGCGCGAGCTCGGCATGTCGCCGTCGGCGTTCCGCCGCAAGGGCGCAGGGCTGAC
GATCGATTACGCGACGGCCGATACGCGGCTTGGCGTCGTGCTCGTCGCGGCGACGAGCAAGGGCATCTGCAAGATCGCGT
TCGGCGATGCCGCAACGCCGCTCGTCGACGAGCTCCGAACGGCGTTCGCGAACGCGCAGATCGTCGAGTCGCCCGCGCGG
ATCGCGCCGTTCATCGCGCAGGTCGATGCGTATCTGAACGGCACGCGCCGGCGCTTCGAGCTGCCGCTCGATCTTGCCGC
GACCGAATTCCAGCAGCGCGTGTGGGATGCGTTGCGTCGGATTCCTTACGGCGAGACGCGCAGCTACACGCAGATCGCCG
AGACGCTCGGCGCACCGCGCTCGGTGCGGGCGGTCGCGAGCGCGTGCGCGTCGAATCCGGTGGCGCTCGCGATCCCGTGC
CACCGCGTCGTGCAGAAGGGCGGCTCGCTTGCCGGCTACCGTTGGGGCTTGCCGCGCAAGGCGGCGCTCCTCGATGCCGA
AGCGCAGCGCGCGGCGCGCGAATCCGTCGAGCCCGCCGTGGACGACGCCGCTTGA

Upstream 100 bases:

>100_bases
CGCGTGACGGAAGTTCGAATGAGAGCGCAAGAAACAGCGCGTGCGCGAGCCGGGTGACGCCTACATTGACAGGCATCCGT
TCCGCTTTTGCGAGGTCATC

Downstream 100 bases:

>100_bases
GCGCCGCCGTCGAATCGGCCGTCGTGCCGTCCGGCAGCGTCGTGTTCGAGCTGCCGTTCAAACCTCCATATGATTGGCCG
CGCGTGCTGCGCTTCTTCGC

Product: ADA regulatory protein

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKQPAYPTDDARWAALVARDADADGAFCYAVKTTGVFCRPSCASRLPRRENVSFFADTDAARAAGYRPCKRCRPEGLPRE
LEIVNRACVVLDAHRQDRFTLAQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRDALQGGAAVTRAAVDAGFNS
PSRLYESVPRELGMSPSAFRRKGAGLTIDYATADTRLGVVLVAATSKGICKIAFGDAATPLVDELRTAFANAQIVESPAR
IAPFIAQVDAYLNGTRRRFELPLDLAATEFQQRVWDALRRIPYGETRSYTQIAETLGAPRSVRAVASACASNPVALAIPC
HRVVQKGGSLAGYRWGLPRKAALLDAEAQRAARESVEPAVDDAA

Sequences:

>Translated_364_residues
MKQPAYPTDDARWAALVARDADADGAFCYAVKTTGVFCRPSCASRLPRRENVSFFADTDAARAAGYRPCKRCRPEGLPRE
LEIVNRACVVLDAHRQDRFTLAQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRDALQGGAAVTRAAVDAGFNS
PSRLYESVPRELGMSPSAFRRKGAGLTIDYATADTRLGVVLVAATSKGICKIAFGDAATPLVDELRTAFANAQIVESPAR
IAPFIAQVDAYLNGTRRRFELPLDLAATEFQQRVWDALRRIPYGETRSYTQIAETLGAPRSVRAVASACASNPVALAIPC
HRVVQKGGSLAGYRWGLPRKAALLDAEAQRAARESVEPAVDDAA
>Mature_364_residues
MKQPAYPTDDARWAALVARDADADGAFCYAVKTTGVFCRPSCASRLPRRENVSFFADTDAARAAGYRPCKRCRPEGLPRE
LEIVNRACVVLDAHRQDRFTLAQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRDALQGGAAVTRAAVDAGFNS
PSRLYESVPRELGMSPSAFRRKGAGLTIDYATADTRLGVVLVAATSKGICKIAFGDAATPLVDELRTAFANAQIVESPAR
IAPFIAQVDAYLNGTRRRFELPLDLAATEFQQRVWDALRRIPYGETRSYTQIAETLGAPRSVRAVASACASNPVALAIPC
HRVVQKGGSLAGYRWGLPRKAALLDAEAQRAARESVEPAVDDAA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=169, Percent_Identity=31.3609467455621, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788542, Length=356, Percent_Identity=46.9101123595506, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=45.4545454545455, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=54.320987654321, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=54.320987654321, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=39.0804597701149, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137554, Length=90, Percent_Identity=46.6666666666667, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39385; Mature: 39385

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPAYPTDDARWAALVARDADADGAFCYAVKTTGVFCRPSCASRLPRRENVSFFADTDA
CCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCEEECCHHHHHCCCCCCCCEEECCCH
ARAAGYRPCKRCRPEGLPRELEIVNRACVVLDAHRQDRFTLAQLSDAVHVSPFHLQRLFK
HHHCCCCCHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHH
RVVGVSPRQYQAAQRGAALRDALQGGAAVTRAAVDAGFNSPSRLYESVPRELGMSPSAFR
HHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHH
RKGAGLTIDYATADTRLGVVLVAATSKGICKIAFGDAATPLVDELRTAFANAQIVESPAR
HCCCCCEEEECCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCCHHHCCCHH
IAPFIAQVDAYLNGTRRRFELPLDLAATEFQQRVWDALRRIPYGETRSYTQIAETLGAPR
HHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCH
SVRAVASACASNPVALAIPCHRVVQKGGSLAGYRWGLPRKAALLDAEAQRAARESVEPAV
HHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCC
DDAA
CCCC
>Mature Secondary Structure
MKQPAYPTDDARWAALVARDADADGAFCYAVKTTGVFCRPSCASRLPRRENVSFFADTDA
CCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCEEECCHHHHHCCCCCCCCEEECCCH
ARAAGYRPCKRCRPEGLPRELEIVNRACVVLDAHRQDRFTLAQLSDAVHVSPFHLQRLFK
HHHCCCCCHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHH
RVVGVSPRQYQAAQRGAALRDALQGGAAVTRAAVDAGFNSPSRLYESVPRELGMSPSAFR
HHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHH
RKGAGLTIDYATADTRLGVVLVAATSKGICKIAFGDAATPLVDELRTAFANAQIVESPAR
HCCCCCEEEECCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCCHHHCCCHH
IAPFIAQVDAYLNGTRRRFELPLDLAATEFQQRVWDALRRIPYGETRSYTQIAETLGAPR
HHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCH
SVRAVASACASNPVALAIPCHRVVQKGGSLAGYRWGLPRKAALLDAEAQRAARESVEPAV
HHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCC
DDAA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]