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The map label for this gene is puuD [H]

Identifier: 83720824

GI number: 83720824

Start: 2052767

End: 2053558

Strand: Direct

Name: puuD [H]

Synonym: BTH_I1827

Alternate gene names: 83720824

Gene position: 2052767-2053558 (Clockwise)

Preceding gene: 83718611

Following gene: 83719641

Centisome position: 53.89

GC content: 73.11

Gene sequence:

>792_bases
ATGAATGAACACCTCGACGCCGGGCCGCGGCCCGTCGTCGGCGTCTGCGCGGATCGAAAGACGGTCGGCCTGCATGTCGC
GCACGTCGCGGGCGAGAAATACGTGAATGCCGTCGTCGACGGCGCAAGCGCGCTCGCAATCGTGCTGCCTGCGCTCGGCG
AGCGGCAGCGCGCGGACGAGCTGCTTGCGCTCGTCGATGGCCTGCTGCTGACGGGCAGCCATTCGAACGTCGAGCCCGCG
CGCTACGGCGGCCCGGCGAGCGCGCCCGGCACGCTGCACGACGCGGCGCGCGACGCGACGACGCTGCCGCTCGTGCGCGC
GGCGATCGACGCCGGCGTGCCCGTGCTCGCGATCTGCCGCGGGATGCAGGAGCTGAACGTCGCGTATGGCGGCACGCTGC
ATCAGGCGGTGCACGCCCTGAGCGGCCATGCCGATCATCGCGAGGACCTGCTGGCATCGGTCGACGTGCAGTACGGCCCC
GCGCATCCGGTCCGGCTCGTGCCGGGCGGCCTGCTGCATCGGCTCGCGGGCGCCGAGGCCGTCGACGTTAATTCTCTGCA
CGCGCAGGGCATCGAGCGGCTCGGCGACGGGCTGACCGTGGAGGCGCGCGCGCCCGACGGGCTCGTCGAGGCGATCGGCG
TGCGCGACGCACGCGCGTTCGCGCTCGGCGTGCAATGGCATCCGGAATGGCGTTTCGACAGCAATCCGCTGTCGCGAGAA
ATCTTTGCGGCGTTCGGCGCGGCGTGCCGCGCCCGAAGACGCCGGACGGCCGGGCGCGCGCCGCGCGCGTGA

Upstream 100 bases:

>100_bases
TGCGCGCAGCCCGCGTGCGACGCCGTTCTTCGTTTTTCGCGACGAACGCCGTGCATTGCGTGCGTTCGCCGCGCGTGTCT
GTCGAATGGGAGAAAGAGCG

Downstream 100 bases:

>100_bases
CGGCCGTCGACGCTTATCGAAGAGAGGCTCACATGCAAGACATCGATGATTTTCTGAGGCAGCACCGGATCACCGAAGTG
GAGGCGATCATCCCCGACAT

Product: glutamine amidotransferase, class I

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNEHLDAGPRPVVGVCADRKTVGLHVAHVAGEKYVNAVVDGASALAIVLPALGERQRADELLALVDGLLLTGSHSNVEPA
RYGGPASAPGTLHDAARDATTLPLVRAAIDAGVPVLAICRGMQELNVAYGGTLHQAVHALSGHADHREDLLASVDVQYGP
AHPVRLVPGGLLHRLAGAEAVDVNSLHAQGIERLGDGLTVEARAPDGLVEAIGVRDARAFALGVQWHPEWRFDSNPLSRE
IFAAFGAACRARRRRTAGRAPRA

Sequences:

>Translated_263_residues
MNEHLDAGPRPVVGVCADRKTVGLHVAHVAGEKYVNAVVDGASALAIVLPALGERQRADELLALVDGLLLTGSHSNVEPA
RYGGPASAPGTLHDAARDATTLPLVRAAIDAGVPVLAICRGMQELNVAYGGTLHQAVHALSGHADHREDLLASVDVQYGP
AHPVRLVPGGLLHRLAGAEAVDVNSLHAQGIERLGDGLTVEARAPDGLVEAIGVRDARAFALGVQWHPEWRFDSNPLSRE
IFAAFGAACRARRRRTAGRAPRA
>Mature_263_residues
MNEHLDAGPRPVVGVCADRKTVGLHVAHVAGEKYVNAVVDGASALAIVLPALGERQRADELLALVDGLLLTGSHSNVEPA
RYGGPASAPGTLHDAARDATTLPLVRAAIDAGVPVLAICRGMQELNVAYGGTLHQAVHALSGHADHREDLLASVDVQYGP
AHPVRLVPGGLLHRLAGAEAVDVNSLHAQGIERLGDGLTVEARAPDGLVEAIGVRDARAFALGVQWHPEWRFDSNPLSRE
IFAAFGAACRARRRRTAGRAPRA

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=243, Percent_Identity=43.6213991769547, Blast_Score=178, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27561; Mature: 27561

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEHLDAGPRPVVGVCADRKTVGLHVAHVAGEKYVNAVVDGASALAIVLPALGERQRADE
CCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
LLALVDGLLLTGSHSNVEPARYGGPASAPGTLHDAARDATTLPLVRAAIDAGVPVLAICR
HHHHHHHHHEECCCCCCCCHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHC
GMQELNVAYGGTLHQAVHALSGHADHREDLLASVDVQYGPAHPVRLVPGGLLHRLAGAEA
CHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEECCHHHHHHHCCCCE
VDVNSLHAQGIERLGDGLTVEARAPDGLVEAIGVRDARAFALGVQWHPEWRFDSNPLSRE
ECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHH
IFAAFGAACRARRRRTAGRAPRA
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNEHLDAGPRPVVGVCADRKTVGLHVAHVAGEKYVNAVVDGASALAIVLPALGERQRADE
CCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
LLALVDGLLLTGSHSNVEPARYGGPASAPGTLHDAARDATTLPLVRAAIDAGVPVLAICR
HHHHHHHHHEECCCCCCCCHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHC
GMQELNVAYGGTLHQAVHALSGHADHREDLLASVDVQYGPAHPVRLVPGGLLHRLAGAEA
CHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEECCHHHHHHHCCCCE
VDVNSLHAQGIERLGDGLTVEARAPDGLVEAIGVRDARAFALGVQWHPEWRFDSNPLSRE
ECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHH
IFAAFGAACRARRRRTAGRAPRA
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]