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The map label for this gene is ywbI [H]

Identifier: 83720703

GI number: 83720703

Start: 25087

End: 26010

Strand: Reverse

Name: ywbI [H]

Synonym: BTH_I0023

Alternate gene names: 83720703

Gene position: 26010-25087 (Counterclockwise)

Preceding gene: 83719057

Following gene: 83718605

Centisome position: 0.68

GC content: 69.05

Gene sequence:

>924_bases
ATGGAGCTGCGCGCGTTGCGTTATTTCGTCGAGGTGGTCAGGCAGCAGAGCTTCACCGTCGCGGCCGAGCAGATGCACGT
CACGCAGCCGACGATCAGCAAGATGGTGAAAGCGCTCGAGGACGAAATCGGCTCGCCGCTCCTGTTGCGCGACGGTCGCC
AGATGGTGCTGACGGACGCCGGCCGGATCGTCTATCAGCGTGGCCAGGACGTGCTCGCCGCGCAGGCGCAGCTCCAGGCC
GAGCTGAACGATCTCGGCACGCTCGGGCGCGGCGAGCTGACGGTCGGGATTCCGCCGCTCGGCGGTTCGCTGTTCACCCC
GGCGATCGCCGCGTTCAAGGCGCGCTATCCGCGAATCGAGCTGAAGTTGTTCGAGCAGGGCGCGCGGAAGATCGAGGAAG
CGCTTGTCGCGGGCGAGCTGGAGCTGGGCGGCGTGCTCGAGCCCGTCGATCCGGCCGTGTTCGACGTGCTGCCGATGGTG
CGTGCGCCGCTGTGGCTCGTCGCGCCGCACGGCTCGCGCTGGGACGCCGACGCGACGGTGCCGCTCGCCGATCTGGCCAA
CGAACCGTTCGTGTTCTATGCGGAAAGCCTCGCGCTGCACGACGCGGTGCTCGACGCGTGCCGCAAGGTCGGCTTCACGC
CGCAGATCGTGAGCCGCAGCGGTCACTGGGATTTCATGGCGGCGCTCGTGCACGCGGGCGTCGGCATCGCGTTGCTGCCC
GAACCGTATTGCCGGCGGCTCGACGCCGCGCAGTTCACGTGCCGGCCGATCGTCGAGCCGGAAATCACATGGGCGATCGC
GATCGGCTGGCTCAAGAAGGGATACCTGTCGCACGCGGCGCGCGCGTGGCTCGACGTCGCACGCGAGACGGAGCCCATCG
ATCCGGGCGACGATCTCGCGTTCGGCAGCCTGCGCGCGCGCTGA

Upstream 100 bases:

>100_bases
AGTATAGGATTCACGCGACCATAAATAAAATGACTTGGTTCTATAAAAACCATTCCGTTTAGGAATCTTTGCCGATTCGC
TCACTGAACAGGAGGCGCTG

Downstream 100 bases:

>100_bases
ACGCGGCGCCCCGGCGAACGGGGTGCTCGCCGCGGCGATCCGGGTTATCCGAAGGGTCGGAACGATCCAGGCTGGCGCGC
CCATCGCATCGCGCGGCCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MELRALRYFVEVVRQQSFTVAAEQMHVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDAGRIVYQRGQDVLAAQAQLQA
ELNDLGTLGRGELTVGIPPLGGSLFTPAIAAFKARYPRIELKLFEQGARKIEEALVAGELELGGVLEPVDPAVFDVLPMV
RAPLWLVAPHGSRWDADATVPLADLANEPFVFYAESLALHDAVLDACRKVGFTPQIVSRSGHWDFMAALVHAGVGIALLP
EPYCRRLDAAQFTCRPIVEPEITWAIAIGWLKKGYLSHAARAWLDVARETEPIDPGDDLAFGSLRAR

Sequences:

>Translated_307_residues
MELRALRYFVEVVRQQSFTVAAEQMHVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDAGRIVYQRGQDVLAAQAQLQA
ELNDLGTLGRGELTVGIPPLGGSLFTPAIAAFKARYPRIELKLFEQGARKIEEALVAGELELGGVLEPVDPAVFDVLPMV
RAPLWLVAPHGSRWDADATVPLADLANEPFVFYAESLALHDAVLDACRKVGFTPQIVSRSGHWDFMAALVHAGVGIALLP
EPYCRRLDAAQFTCRPIVEPEITWAIAIGWLKKGYLSHAARAWLDVARETEPIDPGDDLAFGSLRAR
>Mature_307_residues
MELRALRYFVEVVRQQSFTVAAEQMHVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDAGRIVYQRGQDVLAAQAQLQA
ELNDLGTLGRGELTVGIPPLGGSLFTPAIAAFKARYPRIELKLFEQGARKIEEALVAGELELGGVLEPVDPAVFDVLPMV
RAPLWLVAPHGSRWDADATVPLADLANEPFVFYAESLALHDAVLDACRKVGFTPQIVSRSGHWDFMAALVHAGVGIALLP
EPYCRRLDAAQFTCRPIVEPEITWAIAIGWLKKGYLSHAARAWLDVARETEPIDPGDDLAFGSLRAR

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=296, Percent_Identity=29.0540540540541, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1787879, Length=287, Percent_Identity=29.9651567944251, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1790399, Length=296, Percent_Identity=28.3783783783784, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788887, Length=297, Percent_Identity=31.3131313131313, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI2367136, Length=251, Percent_Identity=27.0916334661355, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693105, Length=284, Percent_Identity=25, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788748, Length=308, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789506, Length=287, Percent_Identity=23.6933797909408, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787530, Length=268, Percent_Identity=24.2537313432836, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=30.0813008130081, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693193, Length=123, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=142, Percent_Identity=33.8028169014084, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=242, Percent_Identity=24.7933884297521, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788296, Length=286, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=122, Percent_Identity=31.1475409836066, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787601, Length=147, Percent_Identity=27.891156462585, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33653; Mature: 33653

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRALRYFVEVVRQQSFTVAAEQMHVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEECC
GRIVYQRGQDVLAAQAQLQAELNDLGTLGRGELTVGIPPLGGSLFTPAIAAFKARYPRIE
CHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCHH
LKLFEQGARKIEEALVAGELELGGVLEPVDPAVFDVLPMVRAPLWLVAPHGSRWDADATV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
PLADLANEPFVFYAESLALHDAVLDACRKVGFTPQIVSRSGHWDFMAALVHAGVGIALLP
CHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCEEECC
EPYCRRLDAAQFTCRPIVEPEITWAIAIGWLKKGYLSHAARAWLDVARETEPIDPGDDLA
CHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FGSLRAR
CCCCCCC
>Mature Secondary Structure
MELRALRYFVEVVRQQSFTVAAEQMHVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEECC
GRIVYQRGQDVLAAQAQLQAELNDLGTLGRGELTVGIPPLGGSLFTPAIAAFKARYPRIE
CHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCHH
LKLFEQGARKIEEALVAGELELGGVLEPVDPAVFDVLPMVRAPLWLVAPHGSRWDADATV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
PLADLANEPFVFYAESLALHDAVLDACRKVGFTPQIVSRSGHWDFMAALVHAGVGIALLP
CHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCEEECC
EPYCRRLDAAQFTCRPIVEPEITWAIAIGWLKKGYLSHAARAWLDVARETEPIDPGDDLA
CHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FGSLRAR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]