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The map label for this gene is cpdB [H]

Identifier: 83720695

GI number: 83720695

Start: 413464

End: 415581

Strand: Reverse

Name: cpdB [H]

Synonym: BTH_I0372

Alternate gene names: 83720695

Gene position: 415581-413464 (Counterclockwise)

Preceding gene: 83718577

Following gene: 83719450

Centisome position: 10.91

GC content: 67.28

Gene sequence:

>2118_bases
ATGCGCCGCTCACGCCATCCCCGGCTCAACGGAAACAGAACAGGAATAATCCCGATGCCATTCCCCTTGCGTTTTCGCCG
GCGGCGCCTAGCCGGCGCCCTCGTCGCATGCGCGGCGCTCGCCGCCGGCTGCAACGACGACGTCGGCTCGCCGCCGCCCG
CATCGTCCGGCGCGCAGACGCCCGCCGGCACGAAGGCGACGCTCGCCGTGCTCGAGACGACCGATCTGCACACGAACGTG
CTGTCGTACGACTACTTCAAGCTCGCGGCCGACAAGTCGCTCGGCTTCGAGCGCGTCGCGACGCTGATCGCGCAGGCGCG
CGCGCAATACCCGAACACGCTGCTGCTCGACAACGGCGACACGATCCAGGGCACGGCGCTCGCCGACTACCAGGCGCTCG
TGAAGCCCGTGTCGTGCGGCGAGACGCTCGCGATCTACAAGGTGATGAACGCGGCGAAGTTCGACGGCGGCGGCATCGGC
AATCACGAATTCAACTACGGGCTGCCGTACCTGTCGCAGGTGACAGGCAACGCGTTCGCCGTCGACGGCCTGCCCGATCC
GGCGCAGCAGAAGAAATGCGCGGGCCCGAACTTCCCGCAGGTGCTCGCGAACGTGATCAGCGCGAAGACGAACGCCCCCC
TCTTCACGCCGTACACGATCCTCACGAAGACCGTCACCGCGACGACGCCCGACGGCCGCACGATCACGGCGCCCGTGAAG
GTCGGCATCATCGGCTTCACGCCGCCCGCGATCATGAGCTGGGACAAGCGCTGGCTCGACGGCAAGGTCTACACGACGGG
CCTGAAGGAAGCCGCCGAGAAGTACATCCCCGAGATGCGCGCGAAGGGCGCGGACCTCGTCGTCGCGATCTCGCACGGCG
GGCTCGACAACTCGCCGTACTCGCCGACGATGGAAAACGGCAGCTGGTGGCTGTCCACCGTGCCCGGCATCGACGCGATG
CTGATCGGCCATTCGCACCAGGTGTTCCCGGATGCGACGAGCACGGTCGCGCAGTTCAACCTGCCGGGCGTCGACAAGGT
CAAGGGCACGGTCAACGGCGTGCCGACCGTGATGGCGAACTACTGGGGCAAGCACCTCGGCGTGATCAAGCTCGGCCTCG
CGTTCGACGGCAAGGCGTGGAGCGTCGACAAATCGCAGACGAGCGTCGAGGCCCGCTCGATCCAGAATCCGGACAAGAGC
TACGTCGACGCCGATCCGTCGGTGGCCGCGGCGATCGCGGCCGAGCATCAGGCGACGATCGACTACGTGAAGACGCCGAT
CGGCGCCACCGACTACCGGATGACCAGCTATTTCGCGGACGTGGGCGACCCGGGCGCGATCCAGATCGTCAACGAGGCGC
AGGCCGACTACGTCGCGAGCTACGTGCGGGCGAACCTGCCACAGTACGCGTCGCTGCCGGTGCTGTCGGTGAGCGCGCCG
TTCAAGAGCGGCTTCGGCGGCGGCGCCGATTTCACCGACGTCGCGGCGGGCGCGCTCGCGATCAACAACGCGGCCGATCT
CTATCTGTATCCGAACACGGTCTACGCGGTGAAGGTGAGCGGCGCGGACATCAAGAACTGGCTCGAGACCGCGGCGAAGC
GCTTCAACACGATCGACCCGACGAAGGCGACCGTGCAGAAGCTCGTGAGCGCGTTCCCCGGCTACAACTTCGACATGTTC
ACGTCCGCCGATCTGCGCTACGAGATCGACGTCACGCAGGCGGCCGGCAGCCGAATCAGGAACCTCACGTACAAGGGCGC
GCCGATCGATCCGAACGCGCAGTTCATCGTCGCGACGAACAACTACCGCGCGAGCGGCGGCGGCAACTTCCCCGGCCTCG
ACGGCAGCAAGACGATCTTCGCTTCGCCCGACGCGAACCGCGACGTGCTGATCGCGTACATCAAGAAGCGCGGCCAGCTC
ACGCGCGCGGCGGACGGCATGCAGCGCAGCTGGCGCTTCACGAAGCTCGCGAGCTCGGTCGCGCACGTGCAGTTCGCGTC
GGCGCCGAACCTCGTCGCGCTCGCGAACGCGGCGAGCCTCGCGGGCATCACGCAAGTCGCGGCCGACGACGGCTCGGGCA
AGGGGCTCGCGACTTACGAGATCGATCTGACGCAATGA

Upstream 100 bases:

>100_bases
TGCTTTCCGCGCGCCTTCCGGTTGGCCGTCCGCGCGCTTTCATTCTTCGGTCACGTTTCCTTTCGATACTCTCACGCGCT
GGATGCCAGGCCGCCGGCGC

Downstream 100 bases:

>100_bases
CGGCGCGGGCGGCGAACGCGCAGCGAGGCTGCGCCGCGCGCCGCCCCGCCCGTCGACCACGAAACCGATTCACCTCGACG
ATGAACCCGACCAAGAATCT

Product: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MRRSRHPRLNGNRTGIIPMPFPLRFRRRRLAGALVACAALAAGCNDDVGSPPPASSGAQTPAGTKATLAVLETTDLHTNV
LSYDYFKLAADKSLGFERVATLIAQARAQYPNTLLLDNGDTIQGTALADYQALVKPVSCGETLAIYKVMNAAKFDGGGIG
NHEFNYGLPYLSQVTGNAFAVDGLPDPAQQKKCAGPNFPQVLANVISAKTNAPLFTPYTILTKTVTATTPDGRTITAPVK
VGIIGFTPPAIMSWDKRWLDGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPYSPTMENGSWWLSTVPGIDAM
LIGHSHQVFPDATSTVAQFNLPGVDKVKGTVNGVPTVMANYWGKHLGVIKLGLAFDGKAWSVDKSQTSVEARSIQNPDKS
YVDADPSVAAAIAAEHQATIDYVKTPIGATDYRMTSYFADVGDPGAIQIVNEAQADYVASYVRANLPQYASLPVLSVSAP
FKSGFGGGADFTDVAAGALAINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDPTKATVQKLVSAFPGYNFDMF
TSADLRYEIDVTQAAGSRIRNLTYKGAPIDPNAQFIVATNNYRASGGGNFPGLDGSKTIFASPDANRDVLIAYIKKRGQL
TRAADGMQRSWRFTKLASSVAHVQFASAPNLVALANAASLAGITQVAADDGSGKGLATYEIDLTQ

Sequences:

>Translated_705_residues
MRRSRHPRLNGNRTGIIPMPFPLRFRRRRLAGALVACAALAAGCNDDVGSPPPASSGAQTPAGTKATLAVLETTDLHTNV
LSYDYFKLAADKSLGFERVATLIAQARAQYPNTLLLDNGDTIQGTALADYQALVKPVSCGETLAIYKVMNAAKFDGGGIG
NHEFNYGLPYLSQVTGNAFAVDGLPDPAQQKKCAGPNFPQVLANVISAKTNAPLFTPYTILTKTVTATTPDGRTITAPVK
VGIIGFTPPAIMSWDKRWLDGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPYSPTMENGSWWLSTVPGIDAM
LIGHSHQVFPDATSTVAQFNLPGVDKVKGTVNGVPTVMANYWGKHLGVIKLGLAFDGKAWSVDKSQTSVEARSIQNPDKS
YVDADPSVAAAIAAEHQATIDYVKTPIGATDYRMTSYFADVGDPGAIQIVNEAQADYVASYVRANLPQYASLPVLSVSAP
FKSGFGGGADFTDVAAGALAINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDPTKATVQKLVSAFPGYNFDMF
TSADLRYEIDVTQAAGSRIRNLTYKGAPIDPNAQFIVATNNYRASGGGNFPGLDGSKTIFASPDANRDVLIAYIKKRGQL
TRAADGMQRSWRFTKLASSVAHVQFASAPNLVALANAASLAGITQVAADDGSGKGLATYEIDLTQ
>Mature_705_residues
MRRSRHPRLNGNRTGIIPMPFPLRFRRRRLAGALVACAALAAGCNDDVGSPPPASSGAQTPAGTKATLAVLETTDLHTNV
LSYDYFKLAADKSLGFERVATLIAQARAQYPNTLLLDNGDTIQGTALADYQALVKPVSCGETLAIYKVMNAAKFDGGGIG
NHEFNYGLPYLSQVTGNAFAVDGLPDPAQQKKCAGPNFPQVLANVISAKTNAPLFTPYTILTKTVTATTPDGRTITAPVK
VGIIGFTPPAIMSWDKRWLDGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPYSPTMENGSWWLSTVPGIDAM
LIGHSHQVFPDATSTVAQFNLPGVDKVKGTVNGVPTVMANYWGKHLGVIKLGLAFDGKAWSVDKSQTSVEARSIQNPDKS
YVDADPSVAAAIAAEHQATIDYVKTPIGATDYRMTSYFADVGDPGAIQIVNEAQADYVASYVRANLPQYASLPVLSVSAP
FKSGFGGGADFTDVAAGALAINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDPTKATVQKLVSAFPGYNFDMF
TSADLRYEIDVTQAAGSRIRNLTYKGAPIDPNAQFIVATNNYRASGGGNFPGLDGSKTIFASPDANRDVLIAYIKKRGQL
TRAADGMQRSWRFTKLASSVAHVQFASAPNLVALANAASLAGITQVAADDGSGKGLATYEIDLTQ

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=663, Percent_Identity=42.2322775263952, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1786687, Length=599, Percent_Identity=22.8714524207012, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 74830; Mature: 74830

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSRHPRLNGNRTGIIPMPFPLRFRRRRLAGALVACAALAAGCNDDVGSPPPASSGAQT
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PAGTKATLAVLETTDLHTNVLSYDYFKLAADKSLGFERVATLIAQARAQYPNTLLLDNGD
CCCCCEEEEEEEECCCHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
TIQGTALADYQALVKPVSCGETLAIYKVMNAAKFDGGGIGNHEFNYGLPYLSQVTGNAFA
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEE
VDGLPDPAQQKKCAGPNFPQVLANVISAKTNAPLFTPYTILTKTVTATTPDGRTITAPVK
ECCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHEEEEEEEECCCCCEEEEEEE
VGIIGFTPPAIMSWDKRWLDGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPY
EEEEECCCHHHHCCCHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
SPTMENGSWWLSTVPGIDAMLIGHSHQVFPDATSTVAQFNLPGVDKVKGTVNGVPTVMAN
CCCCCCCCEEEEECCCCEEEEECCCCCCCCCCHHHHEECCCCCHHHHCCCCCCCHHHHHH
YWGKHLGVIKLGLAFDGKAWSVDKSQTSVEARSIQNPDKSYVDADPSVAAAIAAEHQATI
HHHHHCCEEEEEEEECCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
DYVKTPIGATDYRMTSYFADVGDPGAIQIVNEAQADYVASYVRANLPQYASLPVLSVSAP
HHHHCCCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCEEEECCC
FKSGFGGGADFTDVAAGALAINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDP
CCCCCCCCCCHHHHHHHHEEECCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCCCC
TKATVQKLVSAFPGYNFDMFTSADLRYEIDVTQAAGSRIRNLTYKGAPIDPNAQFIVATN
CHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEHHHCHHHHHCEECCCCCCCCCEEEEEEC
NYRASGGGNFPGLDGSKTIFASPDANRDVLIAYIKKRGQLTRAADGMQRSWRFTKLASSV
CEECCCCCCCCCCCCCCEEEECCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHH
AHVQFASAPNLVALANAASLAGITQVAADDGSGKGLATYEIDLTQ
HHEEECCCCCEEEEECCHHHHHHHHEECCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MRRSRHPRLNGNRTGIIPMPFPLRFRRRRLAGALVACAALAAGCNDDVGSPPPASSGAQT
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PAGTKATLAVLETTDLHTNVLSYDYFKLAADKSLGFERVATLIAQARAQYPNTLLLDNGD
CCCCCEEEEEEEECCCHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
TIQGTALADYQALVKPVSCGETLAIYKVMNAAKFDGGGIGNHEFNYGLPYLSQVTGNAFA
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEE
VDGLPDPAQQKKCAGPNFPQVLANVISAKTNAPLFTPYTILTKTVTATTPDGRTITAPVK
ECCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHEEEEEEEECCCCCEEEEEEE
VGIIGFTPPAIMSWDKRWLDGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPY
EEEEECCCHHHHCCCHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
SPTMENGSWWLSTVPGIDAMLIGHSHQVFPDATSTVAQFNLPGVDKVKGTVNGVPTVMAN
CCCCCCCCEEEEECCCCEEEEECCCCCCCCCCHHHHEECCCCCHHHHCCCCCCCHHHHHH
YWGKHLGVIKLGLAFDGKAWSVDKSQTSVEARSIQNPDKSYVDADPSVAAAIAAEHQATI
HHHHHCCEEEEEEEECCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
DYVKTPIGATDYRMTSYFADVGDPGAIQIVNEAQADYVASYVRANLPQYASLPVLSVSAP
HHHHCCCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCEEEECCC
FKSGFGGGADFTDVAAGALAINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDP
CCCCCCCCCCHHHHHHHHEEECCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCCCC
TKATVQKLVSAFPGYNFDMFTSADLRYEIDVTQAAGSRIRNLTYKGAPIDPNAQFIVATN
CHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEHHHCHHHHHCEECCCCCCCCCEEEEEEC
NYRASGGGNFPGLDGSKTIFASPDANRDVLIAYIKKRGQLTRAADGMQRSWRFTKLASSV
CEECCCCCCCCCCCCCCEEEECCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHH
AHVQFASAPNLVALANAASLAGITQVAADDGSGKGLATYEIDLTQ
HHEEECCCCCEEEEECCHHHHHHHHEECCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005231; 7610040; 9278503; 2172762; 9298646 [H]