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The map label for this gene is ntrB [H]

Identifier: 83720659

GI number: 83720659

Start: 2076391

End: 2077578

Strand: Direct

Name: ntrB [H]

Synonym: BTH_I1845

Alternate gene names: 83720659

Gene position: 2076391-2077578 (Clockwise)

Preceding gene: 83718477

Following gene: 83721563

Centisome position: 54.51

GC content: 66.08

Gene sequence:

>1188_bases
TTGTTCCGCCCGCGACGGCGGTTTTTGACACGGCATCCACGCAAGATGGTTCTGAAGAATCTGATCAAGGCCAGGGCCGG
CCACCCGGAGCGCTTGTCGGACGACGACCGGCTCGTGCGCTCGGGGCTCCTCACGGGGCTGGAGGCGTTGCCGACGGTCG
TGCTCGTGCTCGACCGGCGCACGCTCAGGATCGCGTTCGCCAATCCGTCGGCGGAGGCGATGCTCGACATCTCGCGCCGG
CAGTTGTCGCAGATGCCGTGGAGCGAGATCTTCCCGAACGCGAACGAGCTCGCGACGACGATCGCCGCGATCGGCGAAGA
GCGCTTTCATGCGACGCATCTCGACACCGTGCTCGACCGCCCGGGACGCGAGCCGCTGCACGTGCACGCGATCGTCGGCT
TCCTCGAGGGCGCGCCGGACTTCGTGCTCGTCGAGCTGTTCGAGAACGAGCGGCAGTCGCGCACCGACCGCGAGGAGCGA
ATCCACGATCTCACGGCCGTCAACAAGCAACTGATCCGCAATCTCGCGCACGAGATCAAGAACCCGCTCGGCGGGATTCG
CGGCGCCGCGCAATTGCTCGAATTCGAGTTGGGCGAGCGCGAGCGCGACGAGCTGCGCGAGTACACGCAGGTGATCATCA
AGGAATCGGACCGGCTGCAGACGCTCGTCGACCGTTTGCTCGAACCGCATCGGCACCCGCACATCGTCGGCGACGTGAAT
ATCCATGAAGTGTGCGAGCGGGTGCGCGCCGTGATGCTCGCTGAATTTCCGCGCGGCCTCACGATCGAGCGCGACTACGA
CGTGAGCGTGCCCGACCTGCGCGGCGACAAGGAGCAACTGATCCAGGCGCTGCTGAACATCGTGCGCAACGCGGCGCAGG
CGCTGCGCGAGCGGATCGCGCAGGGCGACGCGAAGATCGAGTTGCGCACGCGCGTCGCGCGCAAGGTGACGATCGCGAAG
CGCCTGTACAGGCTGGCACTGGATTTGCACGTGATCGACAACGGCCCCGGCATTCCCGACGAGATACGCGACCGGATCTT
CTATCCGCTCGTATCCGGCCGCGACGACGGCAGCGGGCTCGGGCTCACGCTCGCGCAGACGTTCATCCAGCAGCACGACG
GGATGATCGAAGTGGACAGCCGGCCGGGCCGGACCGAATTTCAGATTTTGCTGCCGCTCGACCACTGA

Upstream 100 bases:

>100_bases
GTCGGCGGACGAAGCGCCGCGGCGCCGACCGGCCTTCGCGCGGCAACCGCGCGCGAAGGCATCGGACCTTGAAAGGGGAC
GGCGGGACGCCGTCCCCTTT

Downstream 100 bases:

>100_bases
GCGGCGGCGCAGCGATTCACCATACCTCTGACCGACTATGAAGCCGATCTGGATAGTAGACGACGACCAATCGATCCGCT
GGGTGCTCGAGAAGGCGCTC

Product: nitrogen regulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MFRPRRRFLTRHPRKMVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRRTLRIAFANPSAEAMLDISRR
QLSQMPWSEIFPNANELATTIAAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLEGAPDFVLVELFENERQSRTDREER
IHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVN
IHEVCERVRAVMLAEFPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRVARKVTIAK
RLYRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGLGLTLAQTFIQQHDGMIEVDSRPGRTEFQILLPLDH

Sequences:

>Translated_395_residues
MFRPRRRFLTRHPRKMVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRRTLRIAFANPSAEAMLDISRR
QLSQMPWSEIFPNANELATTIAAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLEGAPDFVLVELFENERQSRTDREER
IHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVN
IHEVCERVRAVMLAEFPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRVARKVTIAK
RLYRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGLGLTLAQTFIQQHDGMIEVDSRPGRTEFQILLPLDH
>Mature_395_residues
MFRPRRRFLTRHPRKMVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRRTLRIAFANPSAEAMLDISRR
QLSQMPWSEIFPNANELATTIAAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLEGAPDFVLVELFENERQSRTDREER
IHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVN
IHEVCERVRAVMLAEFPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRVARKVTIAK
RLYRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGLGLTLAQTFIQQHDGMIEVDSRPGRTEFQILLPLDH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=352, Percent_Identity=41.7613636363636, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1788549, Length=354, Percent_Identity=28.5310734463277, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790436, Length=261, Percent_Identity=27.5862068965517, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=26.7489711934156, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87082128, Length=245, Percent_Identity=27.7551020408163, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45154; Mature: 45154

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRPRRRFLTRHPRKMVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRR
CCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TLRIAFANPSAEAMLDISRRQLSQMPWSEIFPNANELATTIAAIGEERFHATHLDTVLDR
EEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGREPLHVHAIVGFLEGAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIK
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
NPLGGIRGAAQLLEFELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCC
IHEVCERVRAVMLAEFPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIA
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGDAKIELRTRVARKVTIAKRLYRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGL
CCCCEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
GLTLAQTFIQQHDGMIEVDSRPGRTEFQILLPLDH
HHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MFRPRRRFLTRHPRKMVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRR
CCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TLRIAFANPSAEAMLDISRRQLSQMPWSEIFPNANELATTIAAIGEERFHATHLDTVLDR
EEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGREPLHVHAIVGFLEGAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIK
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
NPLGGIRGAAQLLEFELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCC
IHEVCERVRAVMLAEFPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIA
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGDAKIELRTRVARKVTIAKRLYRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGL
CCCCEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
GLTLAQTFIQQHDGMIEVDSRPGRTEFQILLPLDH
HHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]