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The map label for this gene is holB [H]

Identifier: 83720641

GI number: 83720641

Start: 2433398

End: 2434432

Strand: Direct

Name: holB [H]

Synonym: BTH_I2155

Alternate gene names: 83720641

Gene position: 2433398-2434432 (Clockwise)

Preceding gene: 83719414

Following gene: 83721488

Centisome position: 63.88

GC content: 71.79

Gene sequence:

>1035_bases
ATGATCTATCCGTGGCAAACCGACGACTGGGCCCGCCTGCAGGCGCTGCGCGCGCATTGGCCGCACGCGCTGTTGCTGTA
CGGCCAGGCGGGCATCGGCAAGCTCGCCTTCGCTCGGCATCTCGCGAAGGGCTTCTTGTGCGAAACGCCGCGCGAGAACG
GCGAGCCGTGCGGCGCGTGCGCCGCGTGCACGTGGTTCGAGCAGGGCAACCACCCCGACTACCGGATCGTCGTGCCGGAG
GCGCTCGCGGCCGAGCTGCCGGGCGCGGCCGACGAGCCGAAGGCCGCCGATGCCGACGAGGGCGGCAAGAAAACCAAGAC
GCCGAGCAAGGAAATCAAGATCGAGCAGGTGCGCGCGCTTGTCGACTTCTGCGGGATCGCGTCGCATCGCGGCGGCGCGC
GCGTCGTCGTGCTGTATCCGGCCGAGGCGCTCAATGTCGCGGCTGCGAACGCGCTGCTCAAGACGCTCGAGGAGCCGCCG
TCGGGCGTCGTGTTCCTGCTCGTGTCCGCGCGCATCGACCGGCTGCTGCCGACGATCGTGAGCCGCTGCCGGCAATGGCC
GATGACGGTGCCCGCACCCGATGCGGCCGCCGCGTGGCTTGCCGCGCAGAACGTGGCGGACGCGAACGCGCTTCTCGCCG
AAGCGGGCGGCGCGCCGCTCGCCGCGCTCGCGCTGGCGAGCGACGAGAACCGGCCGCTGCGCGACTGGACGCTCGCACAG
CTCGCGGCGGGCGCCGCGTGCGATCCGTTCGCGTGCGGCGAGACGCTGCAGAAGCTGCCCGTGCCCGTCGTGCTCGGCTG
GCTGCAGCGCTGGCTGTACGACCTGCTCGCCGAGCGCGCGGCGGGCGCGCCGCGCTATTTCCCCGCGCAGCGCGCGGCGC
TCGCGCGCTGCGCGGCGGCCGTCGACGCGAATGCATTCGCGCGCTTGCTGAAGACGGTCACGCGGCAGCGCGCGGTGGAG
AATCATCCGCTCAGCGCGCGCCTCGTGTTCGAAGAGTTGTTCCTCGGTTATCGCGAATTGTTCGCGTCGTCCTGA

Upstream 100 bases:

>100_bases
CATCGGTTCGTGATCGTCGATTCGAGCGAGCCGATTGCCCAGATCCGCAAGCAGCTCGAAGGCGTGCTCGCCGCGCTGTG
ACGATAGGAAGACGCGAACG

Downstream 100 bases:

>100_bases
CCGACCGTTTCGCCCAGCCGTTTCGTCCATCGGATCGCTTTCATGACCCAGCTTGCCTACCGCGATGCCACGCCCGCCGA
TCTGCCGGCGATCGTCGCCA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIYPWQTDDWARLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGACAACTWFEQGNHPDYRIVVPE
ALAAELPGAADEPKAADADEGGKKTKTPSKEIKIEQVRALVDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPP
SGVVFLLVSARIDRLLPTIVSRCRQWPMTVPAPDAAAAWLAAQNVADANALLAEAGGAPLAALALASDENRPLRDWTLAQ
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAAVDANAFARLLKTVTRQRAVE
NHPLSARLVFEELFLGYRELFASS

Sequences:

>Translated_344_residues
MIYPWQTDDWARLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGACAACTWFEQGNHPDYRIVVPE
ALAAELPGAADEPKAADADEGGKKTKTPSKEIKIEQVRALVDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPP
SGVVFLLVSARIDRLLPTIVSRCRQWPMTVPAPDAAAAWLAAQNVADANALLAEAGGAPLAALALASDENRPLRDWTLAQ
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAAVDANAFARLLKTVTRQRAVE
NHPLSARLVFEELFLGYRELFASS
>Mature_344_residues
MIYPWQTDDWARLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGACAACTWFEQGNHPDYRIVVPE
ALAAELPGAADEPKAADADEGGKKTKTPSKEIKIEQVRALVDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPP
SGVVFLLVSARIDRLLPTIVSRCRQWPMTVPAPDAAAAWLAAQNVADANALLAEAGGAPLAALALASDENRPLRDWTLAQ
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAAVDANAFARLLKTVTRQRAVE
NHPLSARLVFEELFLGYRELFASS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=290, Percent_Identity=33.7931034482759, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37026; Mature: 37026

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPWQTDDWARLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGAC
CCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
AACTWFEQGNHPDYRIVVPEALAAELPGAADEPKAADADEGGKKTKTPSKEIKIEQVRAL
HHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPPSGVVFLLVSARIDRLLPTIV
HHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
SRCRQWPMTVPAPDAAAAWLAAQNVADANALLAEAGGAPLAALALASDENRPLRDWTLAQ
HHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEHEEECCCCCCCHHHHHHH
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAA
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VDANAFARLLKTVTRQRAVENHPLSARLVFEELFLGYRELFASS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYPWQTDDWARLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGAC
CCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
AACTWFEQGNHPDYRIVVPEALAAELPGAADEPKAADADEGGKKTKTPSKEIKIEQVRAL
HHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPPSGVVFLLVSARIDRLLPTIV
HHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
SRCRQWPMTVPAPDAAAAWLAAQNVADANALLAEAGGAPLAALALASDENRPLRDWTLAQ
HHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEHEEECCCCCCCHHHHHHH
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAA
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VDANAFARLLKTVTRQRAVENHPLSARLVFEELFLGYRELFASS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]