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The map label for this gene is iolD [H]

Identifier: 83720606

GI number: 83720606

Start: 3018372

End: 3020375

Strand: Direct

Name: iolD [H]

Synonym: BTH_I2648

Alternate gene names: 83720606

Gene position: 3018372-3020375 (Clockwise)

Preceding gene: 83720109

Following gene: 83718406

Centisome position: 79.24

GC content: 73.0

Gene sequence:

>2004_bases
ATGAACCGCAGAGCAATGGATGACGACACCCTACGGAACCCGAGCGTGAACGACTTCATCGACGACCCGGCCGCGCACCG
CCGCGGCACCGTCCGGCTGACCGCCGCGCAGGCGCTCGTGCGCTACCTGGCCGCGCAGCGCGTCGCGACGGACGGCGGCG
GCACCTCCGCCCACGACGGCGAGCCCCTGTTCGGCGGCGTGTTCGCGATCTTCGGACACGGCAACGTCGCGGGCCTCGGC
GAAGCGCTGCATCAATACCGCGACGCGCTGCCGACCTACCGCGCGCACAACGAGCAGGCGATGGCGCACAGCGCGATCGC
GTACTCGAAGGCGCACATGCGCCGCCGGATGATGGCCGTGACGACGTCGATCGGCCCCGGCGCGACGAACCTCGTCACCG
CGGCCGCGCTCGCGCACGTGAACCGGCTGCCGGTGCTGCTGCTGCCGGGCGACGTGTTCGTGTCGCGCGCGCCGGACCCG
GTGCTGCAGCAGATCGAGGATTTCCACGACGGCGCGATCTCGGCGAACGACGCGTTCAAGGCCGTGTCGCGCTACTTCGA
CCGGATCGTGCATCCGGCCCAGTTGCTCACCGCGCTGCCGCGCGCGCTGCGCGTGCTGACCGACGCGGCGCTCTGCGGCC
CCGTCACGCTCGCGCTGCCGCAGGACGTGCAGGCGATGGCGTACGACTACCCGGCCGAGTTCTTCGCGCCGCGCGCCGTC
GCGTTTCATGCGCCCGCACCCGTCGAGGCGGAGCTCGACGCGGCGCTCGCGCAACTGCGCGCGTCGGCGCGGCCGTTCAT
CGTCGCGGGCGGCGGCGCGCTGTACAGCGAAGGCGGCGCCGCCGCGCTCGCCGCGTTCGCCGAGCGCCACGGCATTCCGG
TCGGCGAGACGCAGGCCGGCAAGAGCGCGCTGCCGTGGGACCACCCGCTGAACGCGGGCGCGATCGGCGTGACGGGCTCG
CCCGCCGCGAACGCGCTCGCGCACGACGCCGATTGCGTGATCGCGCTCGGCACGCGGCTGCAGGATTTCACGACGGGCTC
GAACACGCTGTTCGCGCAGGCGCGCGTCATCGGCATCAACGCGAATGCGTTCGACGCGCTGAAGCATCGCGCGTCGATCG
TGCAGGCCGACGCGCGGCTCGCGCTCGATGCGCTCGGCGCGCGCCTCGAAGGCTGGCGTGCCGACGCCGCGTGGACCGCG
CGCGCGCAAGCGCTTGCCGGCAACTGGCGCGACGCCGTGACGGCCGTCACGCACGCGCCGCCGCGCGCAGGCGCGCTGCC
GTACGACGCCGATGTGATCGGCGCCGTCCAGCGCTCGGCGCCCGATTCGGCCGCGCGCGACATCGCCGTGTGCGCGGCGG
GCACGCTGCCCGCCGAGCTGCACAAGCTCTGGCGCGCGGCGGTGCCAGGCGGCTATCACGTCGAGTACGGCTATTCGTGC
ATGGGCTACGAGATCGCGGGCGGCCTGGGCGCCAAGCTCGCGAAGCCCGAGCGCGACGTGATCGTGATGGTCGGCGACGG
CAGCTATCTGATGATGAACAGCGAGATCGCGACGTCGGTGATGCTCGGCGCGAAGCTGATCGTCGTCGTGCTCGACAACC
GCGGCTACGGCTGCATCAACCGGCTGCAGCAGGCGTGCGGCGGCGCGCCGTTCAACAACCTGTTCGACGACTGCGTGCAA
GGCCCGCTCGGCGCGCCGCGCATCGATTTCGCCGCGCACGCGCGCGCGCTCGGCGCACACGCGGAGCACGTCGCCGACGT
CGCCGCGCTCGAAGCGGCGATGGCGCGCGCCCGCGCGTCCGATCGCACGTACGTGATCTGCATCGATACCGATCCCGCCC
GCACGACGCCCGACGGCGGCTGGTGGTGGGAAGTCGCGGTGCCCGAAGTGTCGCCGCGCGGCGCGGTGCGCGACGCACGC
GCGAACTATGAGCGGCACGTGGCCGCGCGCGCCGCGGCCGCCCCCGCTTCGTCTTCACCCTCTACCCCGGAATCATCGAC
ATGA

Upstream 100 bases:

>100_bases
GAGACGCTGATCGGCGCGTGGCGCGCGGCGCGCGCCGACACCCGCGCGCAGGGCCGGCATGCGGCCGCCGCGCCGCGCGT
GGCCGCCTAGGAGCGCGCGC

Downstream 100 bases:

>100_bases
GCGCACCCGACATCCGTATCGGCATCAATCCGCTGTCGTGGATGAACGACGACCTGCCGTCGCTCGGCGGCGAGACGCCG
CTCGCCGTCGCGCTGACCGA

Product: iolD protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MNRRAMDDDTLRNPSVNDFIDDPAAHRRGTVRLTAAQALVRYLAAQRVATDGGGTSAHDGEPLFGGVFAIFGHGNVAGLG
EALHQYRDALPTYRAHNEQAMAHSAIAYSKAHMRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDP
VLQQIEDFHDGAISANDAFKAVSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQAMAYDYPAEFFAPRAV
AFHAPAPVEAELDAALAQLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGS
PAANALAHDADCVIALGTRLQDFTTGSNTLFAQARVIGINANAFDALKHRASIVQADARLALDALGARLEGWRADAAWTA
RAQALAGNWRDAVTAVTHAPPRAGALPYDADVIGAVQRSAPDSAARDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSC
MGYEIAGGLGAKLAKPERDVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQ
GPLGAPRIDFAAHARALGAHAEHVADVAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRGAVRDAR
ANYERHVAARAAAAPASSSPSTPESST

Sequences:

>Translated_667_residues
MNRRAMDDDTLRNPSVNDFIDDPAAHRRGTVRLTAAQALVRYLAAQRVATDGGGTSAHDGEPLFGGVFAIFGHGNVAGLG
EALHQYRDALPTYRAHNEQAMAHSAIAYSKAHMRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDP
VLQQIEDFHDGAISANDAFKAVSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQAMAYDYPAEFFAPRAV
AFHAPAPVEAELDAALAQLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGS
PAANALAHDADCVIALGTRLQDFTTGSNTLFAQARVIGINANAFDALKHRASIVQADARLALDALGARLEGWRADAAWTA
RAQALAGNWRDAVTAVTHAPPRAGALPYDADVIGAVQRSAPDSAARDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSC
MGYEIAGGLGAKLAKPERDVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQ
GPLGAPRIDFAAHARALGAHAEHVADVAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRGAVRDAR
ANYERHVAARAAAAPASSSPSTPESST
>Mature_667_residues
MNRRAMDDDTLRNPSVNDFIDDPAAHRRGTVRLTAAQALVRYLAAQRVATDGGGTSAHDGEPLFGGVFAIFGHGNVAGLG
EALHQYRDALPTYRAHNEQAMAHSAIAYSKAHMRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDP
VLQQIEDFHDGAISANDAFKAVSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQAMAYDYPAEFFAPRAV
AFHAPAPVEAELDAALAQLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGS
PAANALAHDADCVIALGTRLQDFTTGSNTLFAQARVIGINANAFDALKHRASIVQADARLALDALGARLEGWRADAAWTA
RAQALAGNWRDAVTAVTHAPPRAGALPYDADVIGAVQRSAPDSAARDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSC
MGYEIAGGLGAKLAKPERDVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQ
GPLGAPRIDFAAHARALGAHAEHVADVAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRGAVRDAR
ANYERHVAARAAAAPASSSPSTPESST

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=430, Percent_Identity=26.7441860465116, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081685, Length=508, Percent_Identity=26.3779527559055, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=26.0299625468165, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=22.9965156794425, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69915; Mature: 69915

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRAMDDDTLRNPSVNDFIDDPAAHRRGTVRLTAAQALVRYLAAQRVATDGGGTSAHDG
CCCCCCCCCCCCCCCCHHHCCCCHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
EPLFGGVFAIFGHGNVAGLGEALHQYRDALPTYRAHNEQAMAHSAIAYSKAHMRRRMMAV
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFK
EECCCCCHHHHHHHHHHHHHCCCCEEEECCHHEECCCCCHHHHHHHHHCCCCCCCHHHHH
AVSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQAMAYDYPAEFFAPRAV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCHHHHCCCEE
AFHAPAPVEAELDAALAQLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAG
EECCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHCCCCCCCCCCC
KSALPWDHPLNAGAIGVTGSPAANALAHDADCVIALGTRLQDFTTGSNTLFAQARVIGIN
CCCCCCCCCCCCCEEECCCCCHHHHHHCCCCEEEEECCCHHHCCCCCCEEEEEEEEEEEC
ANAFDALKHRASIVQADARLALDALGARLEGWRADAAWTARAQALAGNWRDAVTAVTHAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCC
PRAGALPYDADVIGAVQRSAPDSAARDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSC
CCCCCCCCCHHHHHHHHHCCCCHHHHCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCEE
MGYEIAGGLGAKLAKPERDVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCIN
ECEEECCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHH
RLQQACGGAPFNNLFDDCVQGPLGAPRIDFAAHARALGAHAEHVADVAALEAAMARARAS
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRGAVRDARANYERHVAARAAAAPASSSP
CCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
STPESST
CCCCCCC
>Mature Secondary Structure
MNRRAMDDDTLRNPSVNDFIDDPAAHRRGTVRLTAAQALVRYLAAQRVATDGGGTSAHDG
CCCCCCCCCCCCCCCCHHHCCCCHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
EPLFGGVFAIFGHGNVAGLGEALHQYRDALPTYRAHNEQAMAHSAIAYSKAHMRRRMMAV
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFK
EECCCCCHHHHHHHHHHHHHCCCCEEEECCHHEECCCCCHHHHHHHHHCCCCCCCHHHHH
AVSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQAMAYDYPAEFFAPRAV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCHHHHCCCEE
AFHAPAPVEAELDAALAQLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAG
EECCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHCCCCCCCCCCC
KSALPWDHPLNAGAIGVTGSPAANALAHDADCVIALGTRLQDFTTGSNTLFAQARVIGIN
CCCCCCCCCCCCCEEECCCCCHHHHHHCCCCEEEEECCCHHHCCCCCCEEEEEEEEEEEC
ANAFDALKHRASIVQADARLALDALGARLEGWRADAAWTARAQALAGNWRDAVTAVTHAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCC
PRAGALPYDADVIGAVQRSAPDSAARDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSC
CCCCCCCCCHHHHHHHHHCCCCHHHHCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCEE
MGYEIAGGLGAKLAKPERDVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCIN
ECEEECCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHH
RLQQACGGAPFNNLFDDCVQGPLGAPRIDFAAHARALGAHAEHVADVAALEAAMARARAS
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRGAVRDARANYERHVAARAAAAPASSSP
CCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
STPESST
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA