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The map label for this gene is alkK [H]

Identifier: 83720533

GI number: 83720533

Start: 2418506

End: 2420275

Strand: Direct

Name: alkK [H]

Synonym: BTH_I2141

Alternate gene names: 83720533

Gene position: 2418506-2420275 (Clockwise)

Preceding gene: 83718409

Following gene: 83721623

Centisome position: 63.49

GC content: 66.78

Gene sequence:

>1770_bases
ATGTTGCTGACCGGTAAGTTAGCCGATATTGTAAGTCCGCGCCGCGCGGGGCGCTCGAAGCCGCCCGCGCGCCGTCGAGC
CGTTTGCAGCAGACCGTATCGGACAAATACTTCAGGGAGAAGGAAGACGATGGGTAAGCCGTTGCTGGGCCAGATGATGG
ATATGCCGCTTCTCGTGTCGTCGCTGATCGCGCATGCGGCGCGGCATGCGGGTGACGTCGAGATCGTCTCGCGGCGCGTC
GAGGGCGACATTCACCGCTATACCTATCGCGATTGCGAGGCGCGTTCCAAACGGCTCGCGCAGGCGCTGACCCGCCTGGG
CGTTGGCGTCGGCGACCGGATTGGAACGCTCGCGTGGAACGGCTACCGGCACGTCGAGGCATATTACGGGATCAGCGGGA
TGGGCGCCGTCTGCCACACGATCAACCCGCGCCTCTTTCCCGAGCAGATCGCCTATATCGTCAATCACGCCGAAGACCGA
TACGTGCTCTTCGACCTCACCTTCGCGCCGCTCGTCGACCAGCTCGCGCCGCAATGTCCGAACGTCAAAGGCTGGATCGC
GATGACGGACGACGCGCATCTGCCGAGGGGCGCGACGCCTTATCTCTGCTACGAGACGCTCGTCGGCGCGCAGGACGGCG
ATTATGCGTGGCCGCTCCTCGACGAGCGGCAGGCGTCGTCGCTTTGCTACACGTCCGGCACGACGGGCCACCCGAAGGGC
GCGCTCTATTCGCACCGCTCGACGGTGCTGCACGCATACGGCGCGGCGCTTCCCGATGCGATGGGGCTGTCGTCGCGCGA
CGCGGCGCTGCCCGTCGTGCCGATGTTCCATGTCAACGCGTGGGGGCTCCCGTACACGGCGGCGCTCACGGGCACGAAGC
TGGTGCTGCCCGGCAAGGACCTCGACGGCAAATCGCTGTACGAACTGATCGAAAGCGAGCGCGTGACGTTCTCGGCGGGC
GTGCCGACCGTCTGGCTCGGCCTGCTCGCATACATGCGCGAGGCCGGCGTGCGCTTCTCGACGCTCGATCGAACGGTGAT
CGGCGGCTCCGCATGCCCGCCGTCGATGCTCGAGACGTTCGAGGACGTCTACGACGTGCGCGTGATTCACGCCTGGGGGA
TGACCGAGCTGTCGCCGCTCGGCACGCTGTCGAAGCTCAACTGGGCGCAGTCGCAGCGCAGCGTCGACGAGCAGCGGCGG
CTCCTCGAGAAGCAGGGCAGGGTGATCTACGGGATCGACATGCGGATCGTCGGCGACGACGGCCGCGAACTGCCGTGGGA
CGGCGTCGCGTTCGGCGACCTGCAGGTGTGCGGGCCGTGGGTGATCGACCGCTATTTCGGGATCGACGCGTCGCCGCTCG
TCGACGGCTGGTTTCCGACGGGCGACGTCGCGACGATCGACGCCGACGGCTTCCTGCAGATCACCGACCGCAGCAAGGAC
GTGATCAAGTCGGGCGGCGAGTGGATCAGCTCGATCGACGTCGAGAACGTCGCGGTCGCGCACCCGGCTGTTGCCGAGGC
CGCGTGCATCGCGTGCGCGCATCCGAAATGGACCGAGCGGCCGCTTCTCGTCGTCGTCAAGCGCGCCGGCATGGACGTGA
CGCGCGGCGAGTTGCTCGCGTTCTATGACGGCAAGGTGGCGAAATGGTGGATTCCGGACGACGTCGTGTTCGTCGACGCG
CTGCCGCACACGGCGACGGGCAAGCTGCAAAAGCTCAAGCTGCGCGAACAGTTCCGGGACCACGTGTTGCCGACGGCGGT
CGACGCGTAG

Upstream 100 bases:

>100_bases
GGCGCCGAGTTGCAGGAACGCGCCGTCGCGCGGGGCGGCGAGCGTCGCGGCGAGATCGGGGAGAGTGGCGGCCGGTGCGG
CCTCGGTTCTGGAAAACGAC

Downstream 100 bases:

>100_bases
ACGCGCGGCCCGCCGGGCGCCCCGCGCGTGCAGGCCGCCGCGTGGGGCGTCACTAAATTGAACGATCGTTCTTTTTTGTT
GTATCCTGCCTCTGTTTCTC

Product: long-chain-fatty-acid--CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MLLTGKLADIVSPRRAGRSKPPARRRAVCSRPYRTNTSGRRKTMGKPLLGQMMDMPLLVSSLIAHAARHAGDVEIVSRRV
EGDIHRYTYRDCEARSKRLAQALTRLGVGVGDRIGTLAWNGYRHVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDR
YVLFDLTFAPLVDQLAPQCPNVKGWIAMTDDAHLPRGATPYLCYETLVGAQDGDYAWPLLDERQASSLCYTSGTTGHPKG
ALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGLPYTAALTGTKLVLPGKDLDGKSLYELIESERVTFSAG
VPTVWLGLLAYMREAGVRFSTLDRTVIGGSACPPSMLETFEDVYDVRVIHAWGMTELSPLGTLSKLNWAQSQRSVDEQRR
LLEKQGRVIYGIDMRIVGDDGRELPWDGVAFGDLQVCGPWVIDRYFGIDASPLVDGWFPTGDVATIDADGFLQITDRSKD
VIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLLVVVKRAGMDVTRGELLAFYDGKVAKWWIPDDVVFVDA
LPHTATGKLQKLKLREQFRDHVLPTAVDA

Sequences:

>Translated_589_residues
MLLTGKLADIVSPRRAGRSKPPARRRAVCSRPYRTNTSGRRKTMGKPLLGQMMDMPLLVSSLIAHAARHAGDVEIVSRRV
EGDIHRYTYRDCEARSKRLAQALTRLGVGVGDRIGTLAWNGYRHVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDR
YVLFDLTFAPLVDQLAPQCPNVKGWIAMTDDAHLPRGATPYLCYETLVGAQDGDYAWPLLDERQASSLCYTSGTTGHPKG
ALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGLPYTAALTGTKLVLPGKDLDGKSLYELIESERVTFSAG
VPTVWLGLLAYMREAGVRFSTLDRTVIGGSACPPSMLETFEDVYDVRVIHAWGMTELSPLGTLSKLNWAQSQRSVDEQRR
LLEKQGRVIYGIDMRIVGDDGRELPWDGVAFGDLQVCGPWVIDRYFGIDASPLVDGWFPTGDVATIDADGFLQITDRSKD
VIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLLVVVKRAGMDVTRGELLAFYDGKVAKWWIPDDVVFVDA
LPHTATGKLQKLKLREQFRDHVLPTAVDA
>Mature_589_residues
MLLTGKLADIVSPRRAGRSKPPARRRAVCSRPYRTNTSGRRKTMGKPLLGQMMDMPLLVSSLIAHAARHAGDVEIVSRRV
EGDIHRYTYRDCEARSKRLAQALTRLGVGVGDRIGTLAWNGYRHVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDR
YVLFDLTFAPLVDQLAPQCPNVKGWIAMTDDAHLPRGATPYLCYETLVGAQDGDYAWPLLDERQASSLCYTSGTTGHPKG
ALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGLPYTAALTGTKLVLPGKDLDGKSLYELIESERVTFSAG
VPTVWLGLLAYMREAGVRFSTLDRTVIGGSACPPSMLETFEDVYDVRVIHAWGMTELSPLGTLSKLNWAQSQRSVDEQRR
LLEKQGRVIYGIDMRIVGDDGRELPWDGVAFGDLQVCGPWVIDRYFGIDASPLVDGWFPTGDVATIDADGFLQITDRSKD
VIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLLVVVKRAGMDVTRGELLAFYDGKVAKWWIPDDVVFVDA
LPHTATGKLQKLKLREQFRDHVLPTAVDA

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=531, Percent_Identity=26.9303201506591, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI187761345, Length=537, Percent_Identity=26.0707635009311, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI187761343, Length=537, Percent_Identity=26.0707635009311, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI28416953, Length=609, Percent_Identity=25.7799671592775, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI115511026, Length=512, Percent_Identity=22.0703125, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI38505220, Length=450, Percent_Identity=24.2222222222222, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI58082049, Length=549, Percent_Identity=23.6794171220401, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI122937307, Length=511, Percent_Identity=22.1135029354207, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157311624, Length=549, Percent_Identity=23.3151183970856, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI157311622, Length=549, Percent_Identity=23.3151183970856, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI221142682, Length=517, Percent_Identity=24.5647969052224, Blast_Score=142, Evalue=9e-35,
Organism=Escherichia coli, GI1788107, Length=531, Percent_Identity=25.6120527306968, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI145693145, Length=520, Percent_Identity=25.3846153846154, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1789201, Length=364, Percent_Identity=25.5494505494505, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786810, Length=356, Percent_Identity=26.123595505618, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=561, Percent_Identity=24.0641711229947, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=561, Percent_Identity=24.0641711229947, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=561, Percent_Identity=24.0641711229947, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17558820, Length=518, Percent_Identity=26.4478764478764, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=510, Percent_Identity=25.8823529411765, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=533, Percent_Identity=25.703564727955, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=541, Percent_Identity=23.6598890942699, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=540, Percent_Identity=24.4444444444444, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=527, Percent_Identity=25.2371916508539, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=513, Percent_Identity=22.8070175438596, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=21.9607843137255, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=364, Percent_Identity=25.5494505494505, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=504, Percent_Identity=22.0238095238095, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=26.9972451790634, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581924, Length=542, Percent_Identity=26.7527675276753, Blast_Score=153, Evalue=4e-37,
Organism=Drosophila melanogaster, GI18859661, Length=518, Percent_Identity=26.4478764478764, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355181, Length=494, Percent_Identity=24.2914979757085, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648260, Length=512, Percent_Identity=22.65625, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281365686, Length=556, Percent_Identity=19.9640287769784, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=550, Percent_Identity=20.9090909090909, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648255, Length=562, Percent_Identity=20.1067615658363, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24653035, Length=502, Percent_Identity=20.3187250996016, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356441, Length=369, Percent_Identity=21.9512195121951, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI161076582, Length=510, Percent_Identity=20.9803921568627, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648257, Length=484, Percent_Identity=22.5206611570248, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21356947, Length=497, Percent_Identity=22.1327967806841, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 64782; Mature: 64782

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTGKLADIVSPRRAGRSKPPARRRAVCSRPYRTNTSGRRKTMGKPLLGQMMDMPLLVS
CCCCCCHHHHCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
SLIAHAARHAGDVEIVSRRVEGDIHRYTYRDCEARSKRLAQALTRLGVGVGDRIGTLAWN
HHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
GYRHVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDLTFAPLVDQLAPQCP
CEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCC
NVKGWIAMTDDAHLPRGATPYLCYETLVGAQDGDYAWPLLDERQASSLCYTSGTTGHPKG
CCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCCCCEEEECCCCCCCCC
ALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGLPYTAALTGTKLVLPGKD
CCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEECCCC
LDGKSLYELIESERVTFSAGVPTVWLGLLAYMREAGVRFSTLDRTVIGGSACPPSMLETF
CCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCHHHHHHH
EDVYDVRVIHAWGMTELSPLGTLSKLNWAQSQRSVDEQRRLLEKQGRVIYGIDMRIVGDD
HHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCC
GRELPWDGVAFGDLQVCGPWVIDRYFGIDASPLVDGWFPTGDVATIDADGFLQITDRSKD
CCCCCCCCEEECCCEECCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCHH
VIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLLVVVKRAGMDVTRGELLA
HHHCCCHHHHHCCCCCEEECCCHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCEEE
FYDGKVAKWWIPDDVVFVDALPHTATGKLQKLKLREQFRDHVLPTAVDA
EECCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLLTGKLADIVSPRRAGRSKPPARRRAVCSRPYRTNTSGRRKTMGKPLLGQMMDMPLLVS
CCCCCCHHHHCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
SLIAHAARHAGDVEIVSRRVEGDIHRYTYRDCEARSKRLAQALTRLGVGVGDRIGTLAWN
HHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
GYRHVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDLTFAPLVDQLAPQCP
CEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCC
NVKGWIAMTDDAHLPRGATPYLCYETLVGAQDGDYAWPLLDERQASSLCYTSGTTGHPKG
CCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCCCCEEEECCCCCCCCC
ALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGLPYTAALTGTKLVLPGKD
CCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEECCCC
LDGKSLYELIESERVTFSAGVPTVWLGLLAYMREAGVRFSTLDRTVIGGSACPPSMLETF
CCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCHHHHHHH
EDVYDVRVIHAWGMTELSPLGTLSKLNWAQSQRSVDEQRRLLEKQGRVIYGIDMRIVGDD
HHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCC
GRELPWDGVAFGDLQVCGPWVIDRYFGIDASPLVDGWFPTGDVATIDADGFLQITDRSKD
CCCCCCCCEEECCCEECCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCHH
VIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLLVVVKRAGMDVTRGELLA
HHHCCCHHHHHCCCCCEEECCCHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCEEE
FYDGKVAKWWIPDDVVFVDALPHTATGKLQKLKLREQFRDHVLPTAVDA
EECCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]