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The map label for this gene is emrB [H]

Identifier: 83720524

GI number: 83720524

Start: 2923525

End: 2925084

Strand: Direct

Name: emrB [H]

Synonym: BTH_I2561

Alternate gene names: 83720524

Gene position: 2923525-2925084 (Clockwise)

Preceding gene: 83720021

Following gene: 83721365

Centisome position: 76.75

GC content: 66.35

Gene sequence:

>1560_bases
ATGGCACAGCCACACGCTCCCCTTCCTCCGCTCAAGGGCGGACAGCTGATACTCGGCACGATCGCGGTATCGCTCGCCGT
GTTCATGAACGTGCTCGACACGTCGATCGCCAATGTCGCGATCCCGACGATCTCGGGCGACCTCGGCGTGTCGTCCGACC
AGGGCACCTGGGTCATCACGTCGTTCGCGGTCGCGAATGCGATCTCGGTGCCGCTCACGGGCTGGCTGACCGATCGCATC
GGTCAGGTGCGCCTGTTCCTCGCGTCGATCATCCTGTTCGTGATCTCGTCCTGGATGTGCGGACTCGCGCCGACGCTGCC
GTTCCTGCTCGCATCGCGCGTGCTGCAGGGCGCGGTCGCCGGCCCGATGATCCCGCTGTCGCAAGCGCTGCTGCTGTCGA
GCTATCCGCGCGCGAAAGCGCCGATGGCGCTCGCGCTCTGGTCGATGACGACGCTGATCGCGCCCGTCGCCGGCCCGATT
CTCGGCGGCTGGATCTCGGACAACTACTCGTGGCCGTGGATCTTCTATGTGAACATTCCGGTCGGCATCGCCGCCGCGGC
CGTCACGTGGATGATCTACCGCAGCCGCGAGTCGGCCGTGCGCCGCGCGCCGATCGACGGCGTCGGGCTCGCCCTTCTCG
TGATCTGGGTCGGCTCGCTGCAGATCATGCTCGACAAGGGCAAGGACCTCGACTGGTTCGCGTCGACGACGATCGTCGTG
CTCGCGCTCACCGCGCTGATCGCGTTCGCGTTCTTCGTCGTCTGGGAATTGACGGCCGAGCATCCGGTCGTCGACCTGTC
GCTGTTCCGGATGCGCAACTTCTCCGGCGGCACGATCGCGCTGTCGGTCGGCTACGGGCTCTACTTCGGCAACCTCGTGC
TGCTGCCGCTGTGGCTTCAGACGCAGATCGGCTACACGGCGACCGACGCGGGGCTCGTGATGGCGCCCGTCGGCTTCTTC
GCGATCCTGCTGTCGCCGCTCACGGGCAAGTTCCTGTCGCGCACCGATCCGCGCTACATTGCAACGGCCGCGTTCCTCAC
GTTCGCGCTGTGCTTCTGGATGCGCTCGCGCTATACGACGGGCGTCGACGAATGGTCGCTGATGGCGCCGACCTTCGTGC
AAGGTATCGCGATGGCGGGCTTCTTCATCCCGCTCGTGTCGATCACGCTGTCCGGTCTGCCCGGCCATCGGATTCCTGCG
GCGTCGGGCCTGTCGAACTTCGTGCGGATCATGTGCGGCGGCATCGGAACGTCGATCTTCCAGACCGCGTGGGACCATCG
GAACAACTTCCACCACGCGCAACTCGTCGAGCAGGCGAACCCGTACAACCCGACGTTCAGCCAGGCGGTCACGCAGATGG
GACAGCTCGGGCTCACGCGCGAGCAAGCGCACGGGCTCATCAACAACATGGCGACGCAGCAGGCCGCGCAACTGGGCGTG
AACGATCTGTTCTACATCTCGGCCGCGATCTTCGTGCTGCTGATCGCGCTCATCTGGATCACGAAGCCCGAGCGCGCGGG
CGGCGGCGATTCGAGCGCGGCGGCGTCGGCCGCGCACTGA

Upstream 100 bases:

>100_bases
GCGGGCGGCGGTGCGCCGACGCCGACGCCGGCGCCGGCGGCGAAGGTCGTCGGCGGCCGCACGTCGAACCTGATGTAAGC
GCCGCCGGGTCGGACCAAGC

Downstream 100 bases:

>100_bases
GCGGCGTCACACGTGGCGCGTCACGCGTGGCGCGCGAAGCAAAACGCCCGGCTTTCAAGCCGGGCGTTTTCGTTTTGCCG
ATGCGACATGCGGCGCCGAG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAQPHAPLPPLKGGQLILGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRI
GQVRLFLASIILFVISSWMCGLAPTLPFLLASRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWSMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAAVTWMIYRSRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIVV
LALTALIAFAFFVVWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGFF
AILLSPLTGKFLSRTDPRYIATAAFLTFALCFWMRSRYTTGVDEWSLMAPTFVQGIAMAGFFIPLVSITLSGLPGHRIPA
ASGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQANPYNPTFSQAVTQMGQLGLTREQAHGLINNMATQQAAQLGV
NDLFYISAAIFVLLIALIWITKPERAGGGDSSAAASAAH

Sequences:

>Translated_519_residues
MAQPHAPLPPLKGGQLILGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRI
GQVRLFLASIILFVISSWMCGLAPTLPFLLASRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWSMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAAVTWMIYRSRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIVV
LALTALIAFAFFVVWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGFF
AILLSPLTGKFLSRTDPRYIATAAFLTFALCFWMRSRYTTGVDEWSLMAPTFVQGIAMAGFFIPLVSITLSGLPGHRIPA
ASGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQANPYNPTFSQAVTQMGQLGLTREQAHGLINNMATQQAAQLGV
NDLFYISAAIFVLLIALIWITKPERAGGGDSSAAASAAH
>Mature_518_residues
AQPHAPLPPLKGGQLILGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRIG
QVRLFLASIILFVISSWMCGLAPTLPFLLASRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWSMTTLIAPVAGPIL
GGWISDNYSWPWIFYVNIPVGIAAAAVTWMIYRSRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIVVL
ALTALIAFAFFVVWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGFFA
ILLSPLTGKFLSRTDPRYIATAAFLTFALCFWMRSRYTTGVDEWSLMAPTFVQGIAMAGFFIPLVSITLSGLPGHRIPAA
SGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQANPYNPTFSQAVTQMGQLGLTREQAHGLINNMATQQAAQLGVN
DLFYISAAIFVLLIALIWITKPERAGGGDSSAAASAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=495, Percent_Identity=54.7474747474747, Blast_Score=540, Evalue=1e-154,
Organism=Escherichia coli, GI1788710, Length=510, Percent_Identity=46.6666666666667, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1790195, Length=376, Percent_Identity=32.1808510638298, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=29.1044776119403, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI87081983, Length=434, Percent_Identity=24.1935483870968, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790146, Length=161, Percent_Identity=29.1925465838509, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=197, Percent_Identity=24.8730964467005, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=147, Percent_Identity=27.2108843537415, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55631; Mature: 55500

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPHAPLPPLKGGQLILGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVIT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEE
SFAVANAISVPLTGWLTDRIGQVRLFLASIILFVISSWMCGLAPTLPFLLASRVLQGAVA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPMIPLSQALLLSSYPRAKAPMALALWSMTTLIAPVAGPILGGWISDNYSWPWIFYVNIP
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
VGIAAAAVTWMIYRSRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIVV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHH
LALTALIAFAFFVVWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQ
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TQIGYTATDAGLVMAPVGFFAILLSPLTGKFLSRTDPRYIATAAFLTFALCFWMRSRYTT
HHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVDEWSLMAPTFVQGIAMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTSIF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTAWDHRNNFHHAQLVEQANPYNPTFSQAVTQMGQLGLTREQAHGLINNMATQQAAQLGV
HHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
NDLFYISAAIFVLLIALIWITKPERAGGGDSSAAASAAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQPHAPLPPLKGGQLILGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVIT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEE
SFAVANAISVPLTGWLTDRIGQVRLFLASIILFVISSWMCGLAPTLPFLLASRVLQGAVA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPMIPLSQALLLSSYPRAKAPMALALWSMTTLIAPVAGPILGGWISDNYSWPWIFYVNIP
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
VGIAAAAVTWMIYRSRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIVV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHH
LALTALIAFAFFVVWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQ
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TQIGYTATDAGLVMAPVGFFAILLSPLTGKFLSRTDPRYIATAAFLTFALCFWMRSRYTT
HHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVDEWSLMAPTFVQGIAMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTSIF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTAWDHRNNFHHAQLVEQANPYNPTFSQAVTQMGQLGLTREQAHGLINNMATQQAAQLGV
HHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
NDLFYISAAIFVLLIALIWITKPERAGGGDSSAAASAAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]