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The map label for this gene is metK [H]

Identifier: 83720507

GI number: 83720507

Start: 204833

End: 206020

Strand: Direct

Name: metK [H]

Synonym: BTH_I0174

Alternate gene names: 83720507

Gene position: 204833-206020 (Clockwise)

Preceding gene: 83718683

Following gene: 83720999

Centisome position: 5.38

GC content: 66.75

Gene sequence:

>1188_bases
GTGGCAAACGATTATCTCTTTACGTCCGAATCCGTTTCCGAAGGCCATCCGGACAAGGTCGCGGACCAAATCTCCGACGC
GATCCTCGACGCAATCCTCGCGCAAGACAAGTATTCCCGAGTCGCGGCCGAAACGCTGTGCAACACCGGCCTCGTCGTGC
TCGCAGGCGAAATCACGACCACGGCGAACATCGACTACATCCAGATCGCGCGCGACACGATCAAGCGCATCGGCTACGAC
AACACCGATTACGGCATCGATTACCGCGGCTGCGCGGTGCTCGTCGCGTATGACAAGCAGTCGCCCGACATCGCGCAAGG
CGTCGACCGCGCGCACGACAACAACCTCGATCAGGGCGCGGGCGACCAGGGCCTGATGTTCGGCTACGCGTGCGACGAAA
CGCCCGAGCTGATGCCGCTGCCGATCCACCTGTCGCACCGGCTCGTCGAGCGCCAGGCGAACCTGCGCCGCGACGGCCGC
CTGCCGTGGCTGCGTCCGGACGCGAAGTCGCAGGTGACGGTGCGCTACGTCGACGGCAAGCCGCACTCGATCGACACCGT
CGTGCTGTCGACGCAGCATGCGCCGGACATCGATCTGCCCGCGCTGCGCGAAGCGGTGATCGAGGAGATCATCAAGCCGA
CGCTGCCCGCCGACCTGATCAAGGGCGACATCAAGTTCCTCGTGAACCCGACCGGCCGCTTCGTGATCGGTGGCCCGCAA
GGCGATTGCGGGCTGACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCGCAGCGCCGCACGGCGGCGGCGCGTTCTC
GGGCAAGGACCCGTCGAAGGTCGACCGCTCGGCCGCCTACGCGGGCCGCTACGTCGCGAAGAACATCGTCGCCGCAGGCC
TTGCGTCGCGCGCGCTGATCCAGGTGTCGTACGCGATCGGCGTCGCCGAGCCGACCTCGGTGATGGTCAACACGTTCGGC
ACGGGCCGCGTGTCCGACGAGACGATCACGAAGCTCGTGCGCGAGCACTTCGACCTGCGTCCGAAGGGCATCATCCAGAT
GCTCGACCTGCTGCGCCCGATCTACGAGAAGACCGCCGCTTACGGCCACTTCGGCCGCGAAGAGCCGGAATTCTCGTGGG
AAGCCGCCGACAAGGCGCTCGCGCTCGCCGAAGCGGCGGGCGTCGAGCCGGCCGTGCAAGTCGCCTGA

Upstream 100 bases:

>100_bases
GCGCCCACGGCGCAGGAAATCCGCTAAAGCGTCGCCACTTCGGGCCTCTGGAAGCTGGCTACGTGAACTTCAACCGTAAC
CACAACAGGAGCCTGAGAAC

Downstream 100 bases:

>100_bases
CGCACGTTCGCGACAGCAAACAAAAACCCCGGTTTAGGCCGGGGTTTTTTATTGTCGGTTGCGCTTCGGGGAAGGCCGCT
CGCGCTGCACGCCGATGTGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYD
NTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGR
LPWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPDIDLPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA

Sequences:

>Translated_395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYD
NTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGR
LPWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPDIDLPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA
>Mature_394_residues
ANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDN
TDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRL
PWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPDIDLPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGT
GRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=58.6842105263158, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=56.8421052631579, Blast_Score=420, Evalue=1e-117,
Organism=Escherichia coli, GI1789311, Length=385, Percent_Identity=69.8701298701299, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=58.7765957446808, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=58.421052631579, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=58.2446808510638, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=55.7840616966581, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.3170731707317, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=52.9255319148936, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=56.6265060240964, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=57.1428571428571, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=58.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=58.5751978891821, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=59.2592592592593, Blast_Score=430, Evalue=1e-121,
Organism=Drosophila melanogaster, GI45552159, Length=378, Percent_Identity=54.4973544973545, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580529, Length=378, Percent_Identity=54.4973544973545, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580537, Length=378, Percent_Identity=54.4973544973545, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580533, Length=378, Percent_Identity=54.4973544973545, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580535, Length=378, Percent_Identity=54.4973544973545, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580531, Length=378, Percent_Identity=54.4973544973545, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=53.9893617021277, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=53.9893617021277, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=51.7241379310345, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=53.781512605042, Blast_Score=132, Evalue=6e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42642; Mature: 42510

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSIDTVVLSTQHAPDIDLPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure 
ANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSIDTVVLSTQHAPDIDLPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA