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The map label for this gene is 83720472

Identifier: 83720472

GI number: 83720472

Start: 3106841

End: 3108319

Strand: Direct

Name: 83720472

Synonym: BTH_I2713

Alternate gene names: NA

Gene position: 3106841-3108319 (Clockwise)

Preceding gene: 83720302

Following gene: 83720868

Centisome position: 81.56

GC content: 63.89

Gene sequence:

>1479_bases
ATGACCAGGAACGCAAGACTCACATGGCAAGGGATCACCGGACTGATCGCGATATGCGCGGCCGCGCTCGCGACCCATCC
TGCACGCGCCGACGTCGCCGCCGGACCGGGCGCATGGAGCAGCCAGCAAACGTGGGCCGCGGACACGGTCAACGGCGGCA
ATCTGACCGGCTACTTCTATTGGCCCGCAACCCAGCCGACGACGCCCAACGGCAAGCGTGCGCTCGTGCTCGTGCTTCAC
GGCTGCCAACAGAGCGCAGCCGGCGACGTCATCGACAATTCGCAAGGCGCGGGCTTCAACTGGAAGAACGTCGCCGATCG
ATATGGCGCGATCATTCTGGCGCCCAACGCGACCGGAAACGTCTATAGCAACCATTGCTGGGACTATGCGAACACGTCGC
CGAATCGCACGAGCGGCCATGTCGGCGTATTGCTCGATCTCATCGGTCGTTTCGTCGGCGACGCGCAGTATGCGATCGAT
CCGAACCAGGTGTACGTGGCGGGCCTGTCTTCCGGTGGCGGCATGACGATGGTGCTCGGCTGCATCGCGCCGGACGTCTT
CGCCGGCATCGGCATCAACGCCGGGCCGCCGCCCGGCACGACCACGCTACAAATCGGATACGTCCCGATCGGCTACACGG
CGCAAACGGCGGCCAACCAGTGCAGGGCGTGGGCGGGCTCGAACGCGGGCAAGTTCTCGACTCAGATCGCCGGCGCCGTA
TGGGGCACGTCGGACTATACCGTGGCCCAGGCGTATGGTCCGCTCGACACCGCCGCCTTTCGGCTCATCTATGGAGGCAC
GTTTGCGCGGGGGGCCACAGTTTCCATTCCCGGCGGAGGCACGAACACGCCTTATACGGACAGCAACGGCAAAGTGCGCA
CGCACGAGATCGTGGTTTCCGGCATGGCCCATGCCTGGCCTGCCGGCGCGGGCGGCAACAACACCCATTACGTGGACGCG
ACGCACATCAACTATCCGGCCTTCGTGATGGACTACTGGGTCAGAAACAACTTACGCGCAAGCGCCGCGCCGACGCAGTC
CGGCACTGCGCCGACAGGGCTGACCGTCACGGCCGCGACGCAGACGAGCGTGTCGCTGTCCTGGAACCCGGTCGCCGACG
CGAGCAGCTACAACGTCTATCGAAACGGCGACAAGGTCGGGGCGTCGACGTCGACGGCCTACATCGATTCCGGCCTGATC
GCCAGCACGACCTATTCCTATACGGTCACCGAGGTCGATCCGAGCGCGGGCGAGAGTGCGCAGTCCTCACCTGTATCGGC
GACAACACAATCGAGTTTCGCCTGCACCGAGACGACGGCCACGAATTATGCGCACGTGCAGGCCGGCCGTGCATACGATT
CATTCGGTATCGCCTACGCGGCCGGCTCGAACCAGAACATGGGGTTCGACAACGTGTTTTATGTGAATACGCTGGCGGAA
ACGAAGGCCGGCTATTACATCATCGGCAATTGTCCGTGA

Upstream 100 bases:

>100_bases
GAAGCGCGACAGATAAGCGAAGCCGTTGCATGAAAAGGCAGGACTGGGAAGCAAAAAGGCCGTGGGTATCGACACCATTC
CCCATTCTAGGAGAGCCAGC

Downstream 100 bases:

>100_bases
ATGCGGTGCACGTGATGTACCTGCGCGGGCATGATTCGCCCAGGTCGATCGACGGGGTGGTGTGATTTTGCGTAAGCGTG
ATACGACAAGACCAGATCGA

Product: poly(3-hydroxybutyrate) depolymerase

Products: NA

Alternate protein names: PHB depolymerase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTRNARLTWQGITGLIAICAAALATHPARADVAAGPGAWSSQQTWAADTVNGGNLTGYFYWPATQPTTPNGKRALVLVLH
GCQQSAAGDVIDNSQGAGFNWKNVADRYGAIILAPNATGNVYSNHCWDYANTSPNRTSGHVGVLLDLIGRFVGDAQYAID
PNQVYVAGLSSGGGMTMVLGCIAPDVFAGIGINAGPPPGTTTLQIGYVPIGYTAQTAANQCRAWAGSNAGKFSTQIAGAV
WGTSDYTVAQAYGPLDTAAFRLIYGGTFARGATVSIPGGGTNTPYTDSNGKVRTHEIVVSGMAHAWPAGAGGNNTHYVDA
THINYPAFVMDYWVRNNLRASAAPTQSGTAPTGLTVTAATQTSVSLSWNPVADASSYNVYRNGDKVGASTSTAYIDSGLI
ASTTYSYTVTEVDPSAGESAQSSPVSATTQSSFACTETTATNYAHVQAGRAYDSFGIAYAAGSNQNMGFDNVFYVNTLAE
TKAGYYIIGNCP

Sequences:

>Translated_492_residues
MTRNARLTWQGITGLIAICAAALATHPARADVAAGPGAWSSQQTWAADTVNGGNLTGYFYWPATQPTTPNGKRALVLVLH
GCQQSAAGDVIDNSQGAGFNWKNVADRYGAIILAPNATGNVYSNHCWDYANTSPNRTSGHVGVLLDLIGRFVGDAQYAID
PNQVYVAGLSSGGGMTMVLGCIAPDVFAGIGINAGPPPGTTTLQIGYVPIGYTAQTAANQCRAWAGSNAGKFSTQIAGAV
WGTSDYTVAQAYGPLDTAAFRLIYGGTFARGATVSIPGGGTNTPYTDSNGKVRTHEIVVSGMAHAWPAGAGGNNTHYVDA
THINYPAFVMDYWVRNNLRASAAPTQSGTAPTGLTVTAATQTSVSLSWNPVADASSYNVYRNGDKVGASTSTAYIDSGLI
ASTTYSYTVTEVDPSAGESAQSSPVSATTQSSFACTETTATNYAHVQAGRAYDSFGIAYAAGSNQNMGFDNVFYVNTLAE
TKAGYYIIGNCP
>Mature_491_residues
TRNARLTWQGITGLIAICAAALATHPARADVAAGPGAWSSQQTWAADTVNGGNLTGYFYWPATQPTTPNGKRALVLVLHG
CQQSAAGDVIDNSQGAGFNWKNVADRYGAIILAPNATGNVYSNHCWDYANTSPNRTSGHVGVLLDLIGRFVGDAQYAIDP
NQVYVAGLSSGGGMTMVLGCIAPDVFAGIGINAGPPPGTTTLQIGYVPIGYTAQTAANQCRAWAGSNAGKFSTQIAGAVW
GTSDYTVAQAYGPLDTAAFRLIYGGTFARGATVSIPGGGTNTPYTDSNGKVRTHEIVVSGMAHAWPAGAGGNNTHYVDAT
HINYPAFVMDYWVRNNLRASAAPTQSGTAPTGLTVTAATQTSVSLSWNPVADASSYNVYRNGDKVGASTSTAYIDSGLIA
STTYSYTVTEVDPSAGESAQSSPVSATTQSSFACTETTATNYAHVQAGRAYDSFGIAYAAGSNQNMGFDNVFYVNTLAET
KAGYYIIGNCP

Specific function: This protein degrades water-insoluble and water-soluble PHB to monomeric D(-)-3-hydroxybutyrate [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00041 fn3 [H]

EC number: =3.1.1.75 [H]

Molecular weight: Translated: 50982; Mature: 50851

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50853 FN3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNARLTWQGITGLIAICAAALATHPARADVAAGPGAWSSQQTWAADTVNGGNLTGYFY
CCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEE
WPATQPTTPNGKRALVLVLHGCQQSAAGDVIDNSQGAGFNWKNVADRYGAIILAPNATGN
ECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCCCCC
VYSNHCWDYANTSPNRTSGHVGVLLDLIGRFVGDAQYAIDPNQVYVAGLSSGGGMTMVLG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCEEEEE
CIAPDVFAGIGINAGPPPGTTTLQIGYVPIGYTAQTAANQCRAWAGSNAGKFSTQIAGAV
HHCHHHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEHEEEEEE
WGTSDYTVAQAYGPLDTAAFRLIYGGTFARGATVSIPGGGTNTPYTDSNGKVRTHEIVVS
ECCCCEEEEEECCCCHHHHEEEEECCCCCCCCEEEECCCCCCCCEECCCCCEEEEEEEEE
GMAHAWPAGAGGNNTHYVDATHINYPAFVMDYWVRNNLRASAAPTQSGTAPTGLTVTAAT
CCHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEC
QTSVSLSWNPVADASSYNVYRNGDKVGASTSTAYIDSGLIASTTYSYTVTEVDPSAGESA
CEEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEECCCEEEEEEEEEEEEEECCCCCCCC
QSSPVSATTQSSFACTETTATNYAHVQAGRAYDSFGIAYAAGSNQNMGFDNVFYVNTLAE
CCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEHHH
TKAGYYIIGNCP
CCCCEEEEECCC
>Mature Secondary Structure 
TRNARLTWQGITGLIAICAAALATHPARADVAAGPGAWSSQQTWAADTVNGGNLTGYFY
CCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEE
WPATQPTTPNGKRALVLVLHGCQQSAAGDVIDNSQGAGFNWKNVADRYGAIILAPNATGN
ECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCCCCC
VYSNHCWDYANTSPNRTSGHVGVLLDLIGRFVGDAQYAIDPNQVYVAGLSSGGGMTMVLG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCEEEEE
CIAPDVFAGIGINAGPPPGTTTLQIGYVPIGYTAQTAANQCRAWAGSNAGKFSTQIAGAV
HHCHHHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEHEEEEEE
WGTSDYTVAQAYGPLDTAAFRLIYGGTFARGATVSIPGGGTNTPYTDSNGKVRTHEIVVS
ECCCCEEEEEECCCCHHHHEEEEECCCCCCCCEEEECCCCCCCCEECCCCCEEEEEEEEE
GMAHAWPAGAGGNNTHYVDATHINYPAFVMDYWVRNNLRASAAPTQSGTAPTGLTVTAAT
CCHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEC
QTSVSLSWNPVADASSYNVYRNGDKVGASTSTAYIDSGLIASTTYSYTVTEVDPSAGESA
CEEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEECCCEEEEEEEEEEEEEECCCCCCCC
QSSPVSATTQSSFACTETTATNYAHVQAGRAYDSFGIAYAAGSNQNMGFDNVFYVNTLAE
CCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEHHH
TKAGYYIIGNCP
CCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644188; 1409594 [H]