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The map label for this gene is paaF-1 [H]

Identifier: 83720428

GI number: 83720428

Start: 3339130

End: 3340428

Strand: Direct

Name: paaF-1 [H]

Synonym: BTH_I2904

Alternate gene names: 83720428

Gene position: 3339130-3340428 (Clockwise)

Preceding gene: 83721405

Following gene: 83720157

Centisome position: 87.66

GC content: 65.51

Gene sequence:

>1299_bases
ATGACAACCTCGCTTCCGCTCGAACCGATCGAACGGGCGAGCCGCGACGAACTGCTCGCGCTTCAGCTCGACCGCCTGAA
ATGGTCACTCGCGCACGCGTACGAGAATTCGCCCGTCTATCGGCGCAAGTTCGACGAGGCGGGCGTGCATCCGACCGATC
TGAAGACGCTGGCGGATCTGGGCCGCTTTCCGTTCACGACGAAGAACGACCTGCGCGACAACTACCCGTTCGGCATGTTC
GCCGTTCCACAGGAGCGCCTCTCGCGCATCCATGCTTCGTCGGGCACGACGGGCAAGCCGACCGTGGTCGGCTACACGGC
GCAAGACATCGATATGTGGGCCGGCCTCGTCGCCCGCTCGATCCGTGCGGCGGGCGCCCGGCCAGGCGACAAGGTCCATG
TGAGCTACGGCTATGGCCTCTTCACGGGCGGTCTCGGCGCCCATTACGGCGCGGAGCGGGCCGGGCTCACGGTGATCCCG
TTCGGCGGCGGCCAGACCGAAAAGCAGGTGCAGCTGATTCAGGACTTCCGGCCCGACATCATCATGGTGACGCCCAGCTA
CATGCTGTCGATCGCCGACGAAATGGAGCGCCAGCACCTCGATCCCGCCCAGTGTTCGCTGCGGATCGGCATCTTCGGCG
CGGAGCCGTGGACCAACGACATGCGCGTCGCGATCGAAAAGCGCATGGGCATCGACGCCGTCGACATCTACGGGCTCTCC
GAAGTGATCGGCCCGGGCGTCGCATCGGAGTGCGTCGAAACGAAGGACGGCCCGACGATCTGGGAAGATCACTTCTACCC
CGAAATCATCGATCCCGACACCGGCGAAGTGCTGCCGGACGGCGAGTTCGGCGAGCTCGTGTTCACGTCGCTCACGAAGG
AGGCAATGCCGATCGTCCGTTATCGGACCCGCGACCTCACGCGCCTGCTGCCCGGCACCGCGCGCACGATGCGCCGGATG
GAAAAGATCACCGGCCGTTCGGACGACATGATGATCGTGCGCGGCGTAAACGTGTTTCCGACCCAGCTCGAAGAGCAGTT
GCTCAAGCAGCGTGCGCTCGCGCCGCACTATCAGGTCGTCCTGACGAAGGACGGGCCGCTCGACGTGCTGACGCTCAACG
TCGAGCCCTGCCCGGAGACGGCGCCGGATGCAGCCGCGATCGATGCCGCACGCCGCTCGCTCGCTCACGACATCAAGTCG
CTGATCGGCGTGACCGCGATCATCAACGTGTTGCCCGTCAACGGCATCGAACGCTCGGTCGGCAAGGCGCGGCGAATCGT
CGACAAGCGGCGGTCGTGA

Upstream 100 bases:

>100_bases
GTCGCGATGTTCCGCGGCAAATCGACCCAAATCAAAGGGACGGTGATCCCTATAGACCGTTAGCGTGCACACGCGCTCAC
ATAAATATATTGGAGACATC

Downstream 100 bases:

>100_bases
CGCTTCGCGCATCGCGCTGGACGCCCCGCGCGCGCCGCCCGTTCCGAAACGAACCGCTTGGAGGAAAAACCGCCGCGCGA
TGCACGGCGGCGCGAATCAC

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTTSLPLEPIERASRDELLALQLDRLKWSLAHAYENSPVYRRKFDEAGVHPTDLKTLADLGRFPFTTKNDLRDNYPFGMF
AVPQERLSRIHASSGTTGKPTVVGYTAQDIDMWAGLVARSIRAAGARPGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIP
FGGGQTEKQVQLIQDFRPDIIMVTPSYMLSIADEMERQHLDPAQCSLRIGIFGAEPWTNDMRVAIEKRMGIDAVDIYGLS
EVIGPGVASECVETKDGPTIWEDHFYPEIIDPDTGEVLPDGEFGELVFTSLTKEAMPIVRYRTRDLTRLLPGTARTMRRM
EKITGRSDDMMIVRGVNVFPTQLEEQLLKQRALAPHYQVVLTKDGPLDVLTLNVEPCPETAPDAAAIDAARRSLAHDIKS
LIGVTAIINVLPVNGIERSVGKARRIVDKRRS

Sequences:

>Translated_432_residues
MTTSLPLEPIERASRDELLALQLDRLKWSLAHAYENSPVYRRKFDEAGVHPTDLKTLADLGRFPFTTKNDLRDNYPFGMF
AVPQERLSRIHASSGTTGKPTVVGYTAQDIDMWAGLVARSIRAAGARPGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIP
FGGGQTEKQVQLIQDFRPDIIMVTPSYMLSIADEMERQHLDPAQCSLRIGIFGAEPWTNDMRVAIEKRMGIDAVDIYGLS
EVIGPGVASECVETKDGPTIWEDHFYPEIIDPDTGEVLPDGEFGELVFTSLTKEAMPIVRYRTRDLTRLLPGTARTMRRM
EKITGRSDDMMIVRGVNVFPTQLEEQLLKQRALAPHYQVVLTKDGPLDVLTLNVEPCPETAPDAAAIDAARRSLAHDIKS
LIGVTAIINVLPVNGIERSVGKARRIVDKRRS
>Mature_431_residues
TTSLPLEPIERASRDELLALQLDRLKWSLAHAYENSPVYRRKFDEAGVHPTDLKTLADLGRFPFTTKNDLRDNYPFGMFA
VPQERLSRIHASSGTTGKPTVVGYTAQDIDMWAGLVARSIRAAGARPGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIPF
GGGQTEKQVQLIQDFRPDIIMVTPSYMLSIADEMERQHLDPAQCSLRIGIFGAEPWTNDMRVAIEKRMGIDAVDIYGLSE
VIGPGVASECVETKDGPTIWEDHFYPEIIDPDTGEVLPDGEFGELVFTSLTKEAMPIVRYRTRDLTRLLPGTARTMRRME
KITGRSDDMMIVRGVNVFPTQLEEQLLKQRALAPHYQVVLTKDGPLDVLTLNVEPCPETAPDAAAIDAARRSLAHDIKSL
IGVTAIINVLPVNGIERSVGKARRIVDKRRS

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=436, Percent_Identity=64.2201834862385, Blast_Score=582, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 47795; Mature: 47664

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSLPLEPIERASRDELLALQLDRLKWSLAHAYENSPVYRRKFDEAGVHPTDLKTLADL
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
GRFPFTTKNDLRDNYPFGMFAVPQERLSRIHASSGTTGKPTVVGYTAQDIDMWAGLVARS
HCCCCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
IRAAGARPGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIPFGGGQTEKQVQLIQDFRPDI
HHHCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCE
IMVTPSYMLSIADEMERQHLDPAQCSLRIGIFGAEPWTNDMRVAIEKRMGIDAVDIYGLS
EEECHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCCCCHHHHHHHHCCCCHHHHCCHH
EVIGPGVASECVETKDGPTIWEDHFYPEIIDPDTGEVLPDGEFGELVFTSLTKEAMPIVR
HHHCCCHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YRTRDLTRLLPGTARTMRRMEKITGRSDDMMIVRGVNVFPTQLEEQLLKQRALAPHYQVV
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
LTKDGPLDVLTLNVEPCPETAPDAAAIDAARRSLAHDIKSLIGVTAIINVLPVNGIERSV
EECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GKARRIVDKRRS
HHHHHHHHHCCC
>Mature Secondary Structure 
TTSLPLEPIERASRDELLALQLDRLKWSLAHAYENSPVYRRKFDEAGVHPTDLKTLADL
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
GRFPFTTKNDLRDNYPFGMFAVPQERLSRIHASSGTTGKPTVVGYTAQDIDMWAGLVARS
HCCCCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
IRAAGARPGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIPFGGGQTEKQVQLIQDFRPDI
HHHCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCE
IMVTPSYMLSIADEMERQHLDPAQCSLRIGIFGAEPWTNDMRVAIEKRMGIDAVDIYGLS
EEECHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCCCCHHHHHHHHCCCCHHHHCCHH
EVIGPGVASECVETKDGPTIWEDHFYPEIIDPDTGEVLPDGEFGELVFTSLTKEAMPIVR
HHHCCCHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YRTRDLTRLLPGTARTMRRMEKITGRSDDMMIVRGVNVFPTQLEEQLLKQRALAPHYQVV
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
LTKDGPLDVLTLNVEPCPETAPDAAAIDAARRSLAHDIKSLIGVTAIINVLPVNGIERSV
EECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GKARRIVDKRRS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]