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The map label for this gene is potH [H]

Identifier: 83720205

GI number: 83720205

Start: 2566214

End: 2567143

Strand: Direct

Name: potH [H]

Synonym: BTH_I2278

Alternate gene names: 83720205

Gene position: 2566214-2567143 (Clockwise)

Preceding gene: 83719844

Following gene: 83720931

Centisome position: 67.37

GC content: 62.47

Gene sequence:

>930_bases
ATGAACGCGCTTTCGTCGCTCATTGCGTGGCCGGTCAGGAAATTCGGCCTGACGGGCAGAACGGCCGTGATCGCGGGCCC
GTTCTTCTGGCTCGTGCTGTTCTTCCTCGTGCCGTTCATCCTCGTCGTCAAGATCAGCTTCGCGGAGCTGCAGCTCGGCA
TTCCGCCGTACACGGAGCTCACCGCGTTTGCCGACGGCGTGCTCAGCATCAAGCTGAACCTGCAGCACTACGCGTTCCTG
TTCAGCGACAGCCTGTACTTCGCGACCTACGTGAACTCGGTCGTCGTCGCTGCCGTCACGACGCTGCTGTGCCTGCTGAT
CGGCTATCCGATGGCGTACTACATCGCGCGCTCGAATCCGGGCACGCGCAACCTGCTGATGATGGCGGTGATGCTGCCGT
TCTGGACGTCGTTCCTGATTCGCGTATACGCGTGGATCGGCATCCTGAAGAACAACGGGCTGCTGAACAACTTCCTGATG
TGGGCGGGGCTCATCAGCACGCCGATCGAGCTGTACCGGACGAACGTCGGCGTCTACATCGGGATGGTCTATTCGTATCT
GCCGTTCCTCGTGATGCCGCTCTACGCGCACCTCGTGAAGATGGACCTGCGCCTGCTCGAGGCCGCTTACGATCTGGGCG
CGAAGCCGTGGAAGGCGTTCGTGCAGATCACGCTGCCGCTGTCGAGGAACGGGATCATCGCGGGCTGCCTGCTCGTGTTC
ATTCCGGCCGTCGGCGAGTACGTGATTCCGGAGCTGCTCGGCGGCGCGAACACGCTGATGATCGGCCGCGTGATGTGGAA
CGAGTTCTTCAACAACGCGGACTGGCCGATGGCGTCGGCCGTGACCTGCGCGATGGTGCTGCTGCTGCTCGTGCCGATGG
CGATGTTCCAGCACTTCCAGGCGAAGGAGCAGGAGGGCCGCCGCAAATGA

Upstream 100 bases:

>100_bases
CGAACGTGTCGAGCCTCGCGATCGCCGATCTCGACTCGCCCGGCTGGGGCGACGAAATCTACGTGCGCTGGAGCGCCGCG
GCCGGCGTGGTGCTGACGTC

Downstream 100 bases:

>100_bases
AGCCGAATCGCTACTTGCAGGTGCTGATGCTCGCGATCGGCTTCGGTTTCCTCTATATCCCGATCGCGAGCCTCGTCGTC
TACTCGTTCAACGAATCGAA

Product: putrescine ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNALSSLIAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFILVVKISFAELQLGIPPYTELTAFADGVLSIKLNLQHYAFL
FSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLM
WAGLISTPIELYRTNVGVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSRNGIIAGCLLVF
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGRRK

Sequences:

>Translated_309_residues
MNALSSLIAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFILVVKISFAELQLGIPPYTELTAFADGVLSIKLNLQHYAFL
FSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLM
WAGLISTPIELYRTNVGVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSRNGIIAGCLLVF
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGRRK
>Mature_309_residues
MNALSSLIAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFILVVKISFAELQLGIPPYTELTAFADGVLSIKLNLQHYAFL
FSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLM
WAGLISTPIELYRTNVGVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSRNGIIAGCLLVF
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGRRK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=285, Percent_Identity=59.2982456140351, Blast_Score=333, Evalue=9e-93,
Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=42.1052631578947, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1787713, Length=255, Percent_Identity=29.0196078431373, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34668; Mature: 34668

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALSSLIAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFILVVKISFAELQLGIPPYTEL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TAFADGVLSIKLNLQHYAFLFSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNP
HHHHCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCC
GTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMWAGLISTPIELYRTNVGVYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSRNGIIAGCLLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHEEEEECCCCCCHHHHHHHHH
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKEQEGRRK
HHHHCCCCC
>Mature Secondary Structure
MNALSSLIAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFILVVKISFAELQLGIPPYTEL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TAFADGVLSIKLNLQHYAFLFSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNP
HHHHCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCC
GTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMWAGLISTPIELYRTNVGVYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSRNGIIAGCLLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHEEEEECCCCCCHHHHHHHHH
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKEQEGRRK
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]