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The map label for this gene is thlA [H]

Identifier: 83720193

GI number: 83720193

Start: 380974

End: 382167

Strand: Reverse

Name: thlA [H]

Synonym: BTH_I0344

Alternate gene names: 83720193

Gene position: 382167-380974 (Counterclockwise)

Preceding gene: 83720993

Following gene: 83719877

Centisome position: 10.03

GC content: 70.94

Gene sequence:

>1194_bases
ATGACGACTGTTGATCAGGACCCGATCGTCATCGCGTCGGCGGCGCGCACGCCGATCGCGGGCTTTCAGGGCGACTTCGC
GTCGCTCGCCGCGCCGCAGCTGGGCGCGGTCGCGATCGCCGCGGCGCTCGAGCGCGCGGGCTTGAAACCGGAGCAGATCG
ACGAGGCGGTGATGGGCTGCGTGCTGCCCGCGGGACAGGGACAGGCCCCGGCGCGGCAGGCCGCGCTCGGCGCGAAGCTG
CCGCTGTCGGTCGGCTGCACGACGGTCAACAAGATGTGCGGCTCGGGCATGCGCGCGGCGATGTTCGCGCACGACATGCT
CATCGCGGGCTCGGTCGACGTGATCGTCGCGGGCGGCATGGAAAGCATGACGAATGCGCCGTACCTGCTGCCGAAGGCGC
GCGCGGGCATGCGCATGGGCCACGGCCAGGTGCTCGACCACATGTTCCTCGACGGCCTCGAGGACGCCTACGACAAGGGC
CGCCTGATGGGCACGTTCGCCGAGGAATGCGCGGGCGAATACGCGTTCTCGCGCGAGGCGCAGGACGCGTTCGCGATCGA
ATCGCTCGCGCGCGCGAAGCGGGCGAACGAGGACGGCTCGTTCGCGTGGGAAATCGCGTCCGTCACGGTGGCCGGCAAGA
AGGGCGACACGGTGGTCGCGCGCGACGAGCAGCCGTTCAAGGCGAACCCCGAGAAGATCCCGACCCTCAAGCCCGCGTTC
AGCAAGACGGGCACCGTGACCGCCGCGAATTCGTCGTCGATCTCCGACGGCGCGGCCGCGCTCGTGATGATGCGCGCGTC
GACGGCGAACAGGCTGGGCGTCACGCCGCTCGCGCGCGTCGTCGGCCATTCGACGTTCGCGCAAGCGCCGTCGAAGTTCA
CGACGGCGCCCGTCGGCGCGATCCGGCGCCTGTTCGACAAGAACGGCTGGCGCGCGGCCGAGGTCGACCTCTACGAGATC
AACGAGGCGTTCGCGGTCGTCACGATGGCGGCGATGAAGGAGCACGGCCTGCCGCACGACAAAGTCAACGTCAACGGCGG
CGCATGCGCGCTCGGCCATCCGATCGGCGCGTCGGGCGCCCGCATCCTCGTCACGCTGATCGGCGCGCTGCGCGCGCGCG
CGGCCAAGCGCGGCGTCGCGAGCCTGTGCATCGGCGGCGGCGAGGCGACCGCGATGGGCATCGAGCTGATCTGA

Upstream 100 bases:

>100_bases
GTCGGCGCGCTCTCGGCAAACGGCGCGCATTCGGCGGACAATGACGTCGTGGCGGCCGATGCGGCCGATCTCATTGCCCA
AACCATCAAGGAGACGAAAC

Downstream 100 bases:

>100_bases
CTTCCGCCGCGCCCCCGAACAGGAGTCCTCCGATGAAGACCGTATTGATCGTCGGCGCATCGCGCGGCATCGGCCGCGAA
TTCGTCGGCCAATATCTGCG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTTVDQDPIVIASAARTPIAGFQGDFASLAAPQLGAVAIAAALERAGLKPEQIDEAVMGCVLPAGQGQAPARQAALGAKL
PLSVGCTTVNKMCGSGMRAAMFAHDMLIAGSVDVIVAGGMESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKG
RLMGTFAEECAGEYAFSREAQDAFAIESLARAKRANEDGSFAWEIASVTVAGKKGDTVVARDEQPFKANPEKIPTLKPAF
SKTGTVTAANSSSISDGAAALVMMRASTANRLGVTPLARVVGHSTFAQAPSKFTTAPVGAIRRLFDKNGWRAAEVDLYEI
NEAFAVVTMAAMKEHGLPHDKVNVNGGACALGHPIGASGARILVTLIGALRARAAKRGVASLCIGGGEATAMGIELI

Sequences:

>Translated_397_residues
MTTVDQDPIVIASAARTPIAGFQGDFASLAAPQLGAVAIAAALERAGLKPEQIDEAVMGCVLPAGQGQAPARQAALGAKL
PLSVGCTTVNKMCGSGMRAAMFAHDMLIAGSVDVIVAGGMESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKG
RLMGTFAEECAGEYAFSREAQDAFAIESLARAKRANEDGSFAWEIASVTVAGKKGDTVVARDEQPFKANPEKIPTLKPAF
SKTGTVTAANSSSISDGAAALVMMRASTANRLGVTPLARVVGHSTFAQAPSKFTTAPVGAIRRLFDKNGWRAAEVDLYEI
NEAFAVVTMAAMKEHGLPHDKVNVNGGACALGHPIGASGARILVTLIGALRARAAKRGVASLCIGGGEATAMGIELI
>Mature_396_residues
TTVDQDPIVIASAARTPIAGFQGDFASLAAPQLGAVAIAAALERAGLKPEQIDEAVMGCVLPAGQGQAPARQAALGAKLP
LSVGCTTVNKMCGSGMRAAMFAHDMLIAGSVDVIVAGGMESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKGR
LMGTFAEECAGEYAFSREAQDAFAIESLARAKRANEDGSFAWEIASVTVAGKKGDTVVARDEQPFKANPEKIPTLKPAFS
KTGTVTAANSSSISDGAAALVMMRASTANRLGVTPLARVVGHSTFAQAPSKFTTAPVGAIRRLFDKNGWRAAEVDLYEIN
EAFAVVTMAAMKEHGLPHDKVNVNGGACALGHPIGASGARILVTLIGALRARAAKRGVASLCIGGGEATAMGIELI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=47.8149100257069, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=45.6632653061224, Blast_Score=315, Evalue=3e-86,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=35.73264781491, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4501853, Length=388, Percent_Identity=35.8247422680412, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=30.4038004750594, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194353979, Length=383, Percent_Identity=28.7206266318538, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1788554, Length=389, Percent_Identity=50.1285347043702, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=43.8144329896907, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787663, Length=397, Percent_Identity=36.272040302267, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI48994986, Length=403, Percent_Identity=36.9727047146402, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788683, Length=417, Percent_Identity=28.0575539568345, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25147385, Length=385, Percent_Identity=41.8181818181818, Blast_Score=297, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=43.1876606683805, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=37.9487179487179, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17535917, Length=389, Percent_Identity=35.73264781491, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=32.541567695962, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=44.9748743718593, Blast_Score=310, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6322031, Length=394, Percent_Identity=31.2182741116751, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24655093, Length=389, Percent_Identity=44.7300771208226, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=45.2442159383033, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI17648125, Length=395, Percent_Identity=35.9493670886076, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=31.1163895486936, Blast_Score=158, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40982; Mature: 40850

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVDQDPIVIASAARTPIAGFQGDFASLAAPQLGAVAIAAALERAGLKPEQIDEAVMGC
CCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VLPAGQGQAPARQAALGAKLPLSVGCTTVNKMCGSGMRAAMFAHDMLIAGSVDVIVAGGM
CCCCCCCCCCHHHHHCCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHEECCEEEEEECCH
ESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKGRLMGTFAEECAGEYAFSREA
HHHCCCCEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCHHHHHHCH
QDAFAIESLARAKRANEDGSFAWEIASVTVAGKKGDTVVARDEQPFKANPEKIPTLKPAF
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCC
SKTGTVTAANSSSISDGAAALVMMRASTANRLGVTPLARVVGHSTFAQAPSKFTTAPVGA
CCCCEEEECCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHCCHHHHHCCCCCCCCHHHH
IRRLFDKNGWRAAEVDLYEINEAFAVVTMAAMKEHGLPHDKVNVNGGACALGHPIGASGA
HHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCCHH
RILVTLIGALRARAAKRGVASLCIGGGEATAMGIELI
HHHHHHHHHHHHHHHHCCCHHEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TTVDQDPIVIASAARTPIAGFQGDFASLAAPQLGAVAIAAALERAGLKPEQIDEAVMGC
CCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VLPAGQGQAPARQAALGAKLPLSVGCTTVNKMCGSGMRAAMFAHDMLIAGSVDVIVAGGM
CCCCCCCCCCHHHHHCCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHEECCEEEEEECCH
ESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKGRLMGTFAEECAGEYAFSREA
HHHCCCCEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCHHHHHHCH
QDAFAIESLARAKRANEDGSFAWEIASVTVAGKKGDTVVARDEQPFKANPEKIPTLKPAF
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCC
SKTGTVTAANSSSISDGAAALVMMRASTANRLGVTPLARVVGHSTFAQAPSKFTTAPVGA
CCCCEEEECCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHCCHHHHHCCCCCCCCHHHH
IRRLFDKNGWRAAEVDLYEINEAFAVVTMAAMKEHGLPHDKVNVNGGACALGHPIGASGA
HHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCCHH
RILVTLIGALRARAAKRGVASLCIGGGEATAMGIELI
HHHHHHHHHHHHHHHHCCCHHEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]