BacMap

Definition	Burkholderia thailandensis E264 chromosome chromosome I, complete sequence.
Accession	NC_007651
Length	3,809,201

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The map label for this gene is livF [H]

Identifier: 83720190

GI number: 83720190

Start: 3782591

End: 3783349

Strand: Reverse

Name: livF [H]

Synonym: BTH_I3321

Alternate gene names: 83720190

Gene position: 3783349-3782591 (Counterclockwise)

Preceding gene: 83721141

Following gene: 83719253

Centisome position: 99.32

GC content: 62.71

Gene sequence:

>759_bases
ATGGCGAATCGGGAGGCGAAGCCGGTTTTGGCGGTGCGGGACTTGTCGGTGCGCTACGGCAAGGTCGAGGCGCTGCATCG
CGCGGCGCTGACGGTGGAAGCGGGGCGGATCGTTACCGTGATCGGCCCGAACGGAGCGGGAAAGTCGACGCTGCTCAATG
CGGTGATGGGGGCATTGCCGACGAATGGCCACGCGACCGGTGCGGTTTACTATCGTGATGACGATGTGAGCGCATTACCG
ATTGAGCAGCGCGTCGTGCTAGGTATGTGTCTCGTGCCCGAAAAGCGTGAGCTGTTCGGCACGATGTCGGTTGAAGACAA
CCTCGTTTTGGGGGCGTACCGGCGGAAGATGGCCGGTGACGCGAACTTCCTGGACCAGCTCGAGCCGGTTTTCTCACTAT
TCCCGAGACTGAAGGAGCGGCGCAAGCAAGCCGCCGGAACGCTATCGGGCGGTGAGCGGCAGATGCTGGCGGTTGGGCGG
GCGCTGATGGGCAAGCCGGATTTGTTGATGCTCGACGAACCGAGCCTCGGGCTCGCGCCGCTGATCGTGAAGGAGATCTT
TCATATCATCAGTGCGCTGCGTGGGACCGGCGTCGCGACGCTGCTGATCGAGCAGAACGCCCGAGCGGCGTTGCAGATCT
CGGACTACGGATACGTGCTCGAAACCGGCGAGATCGTACTGGAAGGCCCGGCGGGCGAGCTTGCGCAGAATCCCCAAGTC
ATCGAAACCTACCTCGGGCTCACGAAGAAAGCGGTGTAG

Upstream 100 bases:

>100_bases
TGGTCGTGATGGAGTTCGGCACGCGGATCGCCGAAGGCCTGCCGCAGGATGTGCAGAAGGACCCGGCGGTGCTGGAAGCT
TATCTCGGTGGGGTGGAATG

Downstream 100 bases:

>100_bases
CAGCGGCTTCGCCAACTCCGGTCGACGGCGTGTTTCACGTGAAACACGCCGTTTTTTGATGGTCGGCCGTTCGAACGAGA
TTCGGGCCAAAACCGAACCC

Product: branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MANREAKPVLAVRDLSVRYGKVEALHRAALTVEAGRIVTVIGPNGAGKSTLLNAVMGALPTNGHATGAVYYRDDDVSALP
IEQRVVLGMCLVPEKRELFGTMSVEDNLVLGAYRRKMAGDANFLDQLEPVFSLFPRLKERRKQAAGTLSGGERQMLAVGR
ALMGKPDLLMLDEPSLGLAPLIVKEIFHIISALRGTGVATLLIEQNARAALQISDYGYVLETGEIVLEGPAGELAQNPQV
IETYLGLTKKAV

Sequences:

>Translated_252_residues
MANREAKPVLAVRDLSVRYGKVEALHRAALTVEAGRIVTVIGPNGAGKSTLLNAVMGALPTNGHATGAVYYRDDDVSALP
IEQRVVLGMCLVPEKRELFGTMSVEDNLVLGAYRRKMAGDANFLDQLEPVFSLFPRLKERRKQAAGTLSGGERQMLAVGR
ALMGKPDLLMLDEPSLGLAPLIVKEIFHIISALRGTGVATLLIEQNARAALQISDYGYVLETGEIVLEGPAGELAQNPQV
IETYLGLTKKAV
>Mature_251_residues
ANREAKPVLAVRDLSVRYGKVEALHRAALTVEAGRIVTVIGPNGAGKSTLLNAVMGALPTNGHATGAVYYRDDDVSALPI
EQRVVLGMCLVPEKRELFGTMSVEDNLVLGAYRRKMAGDANFLDQLEPVFSLFPRLKERRKQAAGTLSGGERQMLAVGRA
LMGKPDLLMLDEPSLGLAPLIVKEIFHIISALRGTGVATLLIEQNARAALQISDYGYVLETGEIVLEGPAGELAQNPQVI
ETYLGLTKKAV

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27262624, Length=159, Percent_Identity=30.188679245283, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27262626, Length=159, Percent_Identity=30.188679245283, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27436953, Length=233, Percent_Identity=27.8969957081545, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI153792144, Length=173, Percent_Identity=32.3699421965318, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI47078218, Length=191, Percent_Identity=30.8900523560209, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45446740, Length=191, Percent_Identity=30.8900523560209, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27881501, Length=215, Percent_Identity=26.5116279069767, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI30795238, Length=215, Percent_Identity=26.5116279069767, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI46592898, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592915, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592978, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592964, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592956, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592971, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI105990541, Length=208, Percent_Identity=29.3269230769231, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI21536376, Length=235, Percent_Identity=26.8085106382979, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI148612844, Length=223, Percent_Identity=29.1479820627803, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI255708477, Length=245, Percent_Identity=28.9795918367347, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI87082268, Length=241, Percent_Identity=46.4730290456432, Blast_Score=210, Evalue=7e-56,
Organism=Escherichia coli, GI1789593, Length=237, Percent_Identity=32.0675105485232, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789864, Length=255, Percent_Identity=31.3725490196078, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787792, Length=258, Percent_Identity=28.6821705426357, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790162, Length=242, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788053, Length=173, Percent_Identity=34.6820809248555, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789672, Length=255, Percent_Identity=25.4901960784314, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081782, Length=211, Percent_Identity=32.7014218009479, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081709, Length=242, Percent_Identity=27.6859504132231, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786253, Length=246, Percent_Identity=28.4552845528455, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790190, Length=210, Percent_Identity=30.4761904761905, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081791, Length=232, Percent_Identity=27.1551724137931, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994943, Length=257, Percent_Identity=29.9610894941634, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787029, Length=241, Percent_Identity=25.3112033195021, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787370, Length=227, Percent_Identity=27.3127753303965, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787089, Length=239, Percent_Identity=28.4518828451883, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790525, Length=249, Percent_Identity=26.5060240963855, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1789991, Length=212, Percent_Identity=26.8867924528302, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788761, Length=220, Percent_Identity=25.9090909090909, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786872, Length=237, Percent_Identity=27.4261603375527, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789586, Length=238, Percent_Identity=28.9915966386555, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788472, Length=218, Percent_Identity=27.0642201834862, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787105, Length=244, Percent_Identity=27.8688524590164, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786563, Length=218, Percent_Identity=28.8990825688073, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48995001, Length=253, Percent_Identity=28.0632411067194, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787712, Length=222, Percent_Identity=30.6306306306306, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788897, Length=230, Percent_Identity=27.8260869565217, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788210, Length=215, Percent_Identity=30.2325581395349, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790467, Length=235, Percent_Identity=27.2340425531915, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787548, Length=256, Percent_Identity=26.171875, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87082267, Length=157, Percent_Identity=31.8471337579618, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786398, Length=231, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788450, Length=217, Percent_Identity=26.7281105990783, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788644, Length=247, Percent_Identity=28.3400809716599, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786319, Length=225, Percent_Identity=30.2222222222222, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI48994883, Length=250, Percent_Identity=25.2, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789032, Length=225, Percent_Identity=29.3333333333333, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786698, Length=173, Percent_Identity=32.3699421965318, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790739, Length=246, Percent_Identity=25.2032520325203, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788165, Length=248, Percent_Identity=26.2096774193548, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081834, Length=219, Percent_Identity=26.9406392694064, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983516, Length=203, Percent_Identity=29.5566502463054, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=203, Percent_Identity=29.5566502463054, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=203, Percent_Identity=29.5566502463054, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=177, Percent_Identity=32.2033898305085, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=169, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115532730, Length=198, Percent_Identity=28.7878787878788, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193209708, Length=239, Percent_Identity=30.1255230125523, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=239, Percent_Identity=24.6861924686192, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193208177, Length=225, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193202349, Length=262, Percent_Identity=26.7175572519084, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322640, Length=238, Percent_Identity=25.6302521008403, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24666092, Length=186, Percent_Identity=30.1075268817204, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI221512771, Length=230, Percent_Identity=26.5217391304348, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19920532, Length=215, Percent_Identity=27.4418604651163, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24580930, Length=215, Percent_Identity=27.4418604651163, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574533, Length=244, Percent_Identity=25.4098360655738, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24643674, Length=222, Percent_Identity=24.7747747747748, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574150, Length=202, Percent_Identity=25.2475247524752, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI116007328, Length=202, Percent_Identity=25.2475247524752, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24662800, Length=196, Percent_Identity=29.5918367346939, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI85724864, Length=212, Percent_Identity=26.8867924528302, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24580555, Length=212, Percent_Identity=26.8867924528302, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI85816269, Length=212, Percent_Identity=26.8867924528302, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580553, Length=212, Percent_Identity=26.8867924528302, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85724858, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI85724856, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24580547, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24580551, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24580549, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24580545, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27074; Mature: 26943

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANREAKPVLAVRDLSVRYGKVEALHRAALTVEAGRIVTVIGPNGAGKSTLLNAVMGALP
CCCCCCCCEEEEECCHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCHHHHHHHHHHHCC
TNGHATGAVYYRDDDVSALPIEQRVVLGMCLVPEKRELFGTMSVEDNLVLGAYRRKMAGD
CCCCCEEEEEEECCCCCCCCHHHHHHHHEECCCCHHHHEEECCCCCCEEEHHHHHHHCCC
ANFLDQLEPVFSLFPRLKERRKQAAGTLSGGERQMLAVGRALMGKPDLLMLDEPSLGLAP
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHH
LIVKEIFHIISALRGTGVATLLIEQNARAALQISDYGYVLETGEIVLEGPAGELAQNPQV
HHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEECCCEEEECCCCHHHCCCHH
IETYLGLTKKAV
HHHHHHHHHCCC
>Mature Secondary Structure 
ANREAKPVLAVRDLSVRYGKVEALHRAALTVEAGRIVTVIGPNGAGKSTLLNAVMGALP
CCCCCCCEEEEECCHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCHHHHHHHHHHHCC
TNGHATGAVYYRDDDVSALPIEQRVVLGMCLVPEKRELFGTMSVEDNLVLGAYRRKMAGD
CCCCCEEEEEEECCCCCCCCHHHHHHHHEECCCCHHHHEEECCCCCCEEEHHHHHHHCCC
ANFLDQLEPVFSLFPRLKERRKQAAGTLSGGERQMLAVGRALMGKPDLLMLDEPSLGLAP
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHH
LIVKEIFHIISALRGTGVATLLIEQNARAALQISDYGYVLETGEIVLEGPAGELAQNPQV
HHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEECCCEEEECCCCHHHCCCHH
IETYLGLTKKAV
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]