| Definition | Burkholderia thailandensis E264 chromosome chromosome I, complete sequence. |
|---|---|
| Accession | NC_007651 |
| Length | 3,809,201 |
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The map label for this gene is plcN [H]
Identifier: 83720180
GI number: 83720180
Start: 348389
End: 350617
Strand: Reverse
Name: plcN [H]
Synonym: BTH_I0317
Alternate gene names: 83720180
Gene position: 350617-348389 (Counterclockwise)
Preceding gene: 83720177
Following gene: 83721621
Centisome position: 9.2
GC content: 68.06
Gene sequence:
>2229_bases ATGGCGCCGACGACGCCCCCGGCGCAACGGCGGCGTCCGCGCAACCCGCCTCGAATCCAGGCTGATCGCTTTTTGCCGCC CCCTCTTCCCCGCAGAGTCAAAGGTGAAAACATGACGTCAGAAAGCCGCCGCCGTTTCCTGCATGCCGTTGCGCAATCCG CGAGCGCCGCCGCCGCGCTCACCGTGTTTCCCGAATCGATCCGCCGCGCGCTCGCGATTCCCGCCGCGTCGCGCACGGGC ACGATCCGCGATGTCGAGCACATCGTCGTGTTCATGCAGGAAAACCGCTCGTTCGACCACTACTTCGGACACCTGCGCGG CGTGCGCGGCTACAACGACCGCTTCCCGATCACGCTGCCGAACGGCAAACCGGTCTGGTATCAGCCGTCGAAGGCGAACC CGGGCAAGCCGGTGCTGCCGTTTCGCCTGAACACGCTGACGACGAGCGCGCAGTGCATCGGCGACCTCGATCACTCGTGG TACAAGACGCACGCGGCGATCGACGGCGGCCGCTACGACCAGTGGCCCGCGAACAAGACCGACATGACGATGGGCTATCA CGTGCGCGAGGACATTCCGTTCCACTACGCGCTCGCCGATGCATTCACCGTCTGCGACAACTACTTCTGCTCGCTGCCCG GCCCGACGCATCCGAACCGCTCGTATCTGATGACGGGCACCGTCGACCCAACCGGCAAGTACGGCGGCCCGCTTCTCGAC AACAGCGATTACGTCGACGGCGACGCGCCGCCGAAATACGACTTGCTCACGTGGACCACGTACCCCGAGCGCCTCGAGGC GAACGGCATCTCGTGGCAGATCTACCAGCAAGGCACGACGGGCGCCGATCCGCTGAACGGCAACTACGGGACGAACATCC TGCAGAACTTCGCGAACTTCATCGACGCGAAGCCGGGCTCGTCGCTGTATCGGCGCGCGCAGACGGCGCGCACGCTCGAC GACCTGAAGGCCGACGTGCTCGCGAACAGGCTGCCGCAGGTGTCGTGGCTGCTGCCGCCCGCGGCATTCTCCGAGCATCC GAAATACACGCCCGCGTACGGCGCGAACTACACGTCGCAAATCCTCGACGCGCTGACGTCGAACCCCGACGTGTGGCGCA AGACCGTGCTCTTCATCATGTACGACGAGAACGACGGCTTCTTCGATCACGTCGTGCCGCCGCAGCCGGCGACGACGCGC GCGCAGGGCCTGTCGACCGTGCCCGTCGACGGCGAGATCCACGACGTCGTGAACCCGGGCCGCGGCGGCAGCTACACGGC CGACGGCCTGCCGTACGGCCTCGGCCCGCGCGTGCCGATGACGGTCGTGTCGCCGTGGACGAAGGGCGGCTTCGTGTGCT CGCAGGTGTTCGACCATACGTCGGTGATCCGCTTCATCGCCGCGCGCTTCGGCGTCGACGAGCCGAACATCACGCCGTGG CGCCGCGCGGTGTGCGGCGACCTCACGTCCGCGTTCGACTTCCGCACGCCGGACGCGACACTGCCGCCGCTGCCCGACAC GAGCAACTATCGGTCGATCGCCGACCAGCTCTGCTCGACGAAGCCCGCGCCGAGCGTGCCCGCCGCGCCGAGTCCCGTCG ATCCGCAGGAGCCCGGCGTGCGCCCGGCGCGCGCGCTGCCGTACGAGCTGCACGTGAACGCGACGGTGCACGGCGGCGCG CGCTTGCGCATCGAGTTCGCGAATCGCGGCGAACAGGGCGCGCACTTCTACGTGTACGCGTCGAATCGCAGCGACGGCCC GTGGCGCTACACGGTGGGCGCACACCGCTCGCTGCACGACGAATTCGATCTCGGCGCGACGAACGGCGCGTATGCGTTCA CCGTATATGGTCCGAACGGCTTCGTGCGCGTGTTCGAAGGGCTCGCCGCAGCCGAGGGGCACGGCCGGCATGCGGCCGCG CATCCGGAAGTGAAGGCGGGCTACGACGTCGCGAACGGCAACCTGTATCTGAAGCTCACGAATCACGGCGGACAGAGCGT GCGACTGACGCTCGCCGACAACGCGTACGGCGCGCCGCCGCGCCAGATCACGCTGCGCGGCGGCGACGAGCGGGTGGAGC AATGGGCGCTTGCCCCGAGCCATCACTGGTATGACGTGACGGTGTCGGACGGCGCGACCGGCCAGTTCGTGCGCCGCTTC GCCGGGCACGTCGAAAACGGCAAGTCGAGCTATTCCGATCCGGCAGCAGTCGCGCCCGTCACGGGCTGA
Upstream 100 bases:
>100_bases GGCGCCGACGCGAATGCGTTCAACGGCAACGCGACGGCGGCCGGCGATGCGGGCGCGAGCGCGCCGCTCGCGACGCCCGT GATCCACTACCCGCCCGAAG
Downstream 100 bases:
>100_bases ATAAAGGGGCCCGGGCGGCGGCGCGCGTGAGCGGATGCCGTCGCCCTGTCGATTCGTTCAATCGGTTCGATCGGTTCGAT CGCGCTCGCCCGATCCGTTC
Product: non-hemolytic phospholipase C
Products: NA
Alternate protein names: PLC-N; Phosphatidylcholine cholinephosphohydrolase; Phosphatidylcholine-hydrolyzing phospholipase C; PC-PLC [H]
Number of amino acids: Translated: 742; Mature: 741
Protein sequence:
>742_residues MAPTTPPAQRRRPRNPPRIQADRFLPPPLPRRVKGENMTSESRRRFLHAVAQSASAAAALTVFPESIRRALAIPAASRTG TIRDVEHIVVFMQENRSFDHYFGHLRGVRGYNDRFPITLPNGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLDHSW YKTHAAIDGGRYDQWPANKTDMTMGYHVREDIPFHYALADAFTVCDNYFCSLPGPTHPNRSYLMTGTVDPTGKYGGPLLD NSDYVDGDAPPKYDLLTWTTYPERLEANGISWQIYQQGTTGADPLNGNYGTNILQNFANFIDAKPGSSLYRRAQTARTLD DLKADVLANRLPQVSWLLPPAAFSEHPKYTPAYGANYTSQILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPATTR AQGLSTVPVDGEIHDVVNPGRGGSYTADGLPYGLGPRVPMTVVSPWTKGGFVCSQVFDHTSVIRFIAARFGVDEPNITPW RRAVCGDLTSAFDFRTPDATLPPLPDTSNYRSIADQLCSTKPAPSVPAAPSPVDPQEPGVRPARALPYELHVNATVHGGA RLRIEFANRGEQGAHFYVYASNRSDGPWRYTVGAHRSLHDEFDLGATNGAYAFTVYGPNGFVRVFEGLAAAEGHGRHAAA HPEVKAGYDVANGNLYLKLTNHGGQSVRLTLADNAYGAPPRQITLRGGDERVEQWALAPSHHWYDVTVSDGATGQFVRRF AGHVENGKSSYSDPAAVAPVTG
Sequences:
>Translated_742_residues MAPTTPPAQRRRPRNPPRIQADRFLPPPLPRRVKGENMTSESRRRFLHAVAQSASAAAALTVFPESIRRALAIPAASRTG TIRDVEHIVVFMQENRSFDHYFGHLRGVRGYNDRFPITLPNGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLDHSW YKTHAAIDGGRYDQWPANKTDMTMGYHVREDIPFHYALADAFTVCDNYFCSLPGPTHPNRSYLMTGTVDPTGKYGGPLLD NSDYVDGDAPPKYDLLTWTTYPERLEANGISWQIYQQGTTGADPLNGNYGTNILQNFANFIDAKPGSSLYRRAQTARTLD DLKADVLANRLPQVSWLLPPAAFSEHPKYTPAYGANYTSQILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPATTR AQGLSTVPVDGEIHDVVNPGRGGSYTADGLPYGLGPRVPMTVVSPWTKGGFVCSQVFDHTSVIRFIAARFGVDEPNITPW RRAVCGDLTSAFDFRTPDATLPPLPDTSNYRSIADQLCSTKPAPSVPAAPSPVDPQEPGVRPARALPYELHVNATVHGGA RLRIEFANRGEQGAHFYVYASNRSDGPWRYTVGAHRSLHDEFDLGATNGAYAFTVYGPNGFVRVFEGLAAAEGHGRHAAA HPEVKAGYDVANGNLYLKLTNHGGQSVRLTLADNAYGAPPRQITLRGGDERVEQWALAPSHHWYDVTVSDGATGQFVRRF AGHVENGKSSYSDPAAVAPVTG >Mature_741_residues APTTPPAQRRRPRNPPRIQADRFLPPPLPRRVKGENMTSESRRRFLHAVAQSASAAAALTVFPESIRRALAIPAASRTGT IRDVEHIVVFMQENRSFDHYFGHLRGVRGYNDRFPITLPNGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLDHSWY KTHAAIDGGRYDQWPANKTDMTMGYHVREDIPFHYALADAFTVCDNYFCSLPGPTHPNRSYLMTGTVDPTGKYGGPLLDN SDYVDGDAPPKYDLLTWTTYPERLEANGISWQIYQQGTTGADPLNGNYGTNILQNFANFIDAKPGSSLYRRAQTARTLDD LKADVLANRLPQVSWLLPPAAFSEHPKYTPAYGANYTSQILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPATTRA QGLSTVPVDGEIHDVVNPGRGGSYTADGLPYGLGPRVPMTVVSPWTKGGFVCSQVFDHTSVIRFIAARFGVDEPNITPWR RAVCGDLTSAFDFRTPDATLPPLPDTSNYRSIADQLCSTKPAPSVPAAPSPVDPQEPGVRPARALPYELHVNATVHGGAR LRIEFANRGEQGAHFYVYASNRSDGPWRYTVGAHRSLHDEFDLGATNGAYAFTVYGPNGFVRVFEGLAAAEGHGRHAAAH PEVKAGYDVANGNLYLKLTNHGGQSVRLTLADNAYGAPPRQITLRGGDERVEQWALAPSHHWYDVTVSDGATGQFVRRFA GHVENGKSSYSDPAAVAPVTG
Specific function: Hydrolyzes phosphatidylserine as well as phosphatidylcholine [H]
COG id: COG3511
COG function: function code M; Phospholipase C
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the bacterial phospholipase C family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR007312 - InterPro: IPR008475 - InterPro: IPR017767 - InterPro: IPR006311 [H]
Pfam domain/function: PF05506 DUF756; PF04185 Phosphoesterase [H]
EC number: =3.1.4.3 [H]
Molecular weight: Translated: 81449; Mature: 81317
Theoretical pI: Translated: 7.78; Mature: 7.78
Prosite motif: PS00389 ATPASE_DELTA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.9 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 0.9 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAPTTPPAQRRRPRNPPRIQADRFLPPPLPRRVKGENMTSESRRRFLHAVAQSASAAAAL CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE TVFPESIRRALAIPAASRTGTIRDVEHIVVFMQENRSFDHYFGHLRGVRGYNDRFPITLP EECHHHHHHHHCCCCCCCCCCHHHHEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCEECC NGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLDHSWYKTHAAIDGGRYDQWPANKT CCCEEEECCCCCCCCCCCCCEEECCCCCHHHHHHCCCHHHHHHHEEECCCCCCCCCCCCC DMTMGYHVREDIPFHYALADAFTVCDNYFCSLPGPTHPNRSYLMTGTVDPTGKYGGPLLD CEEECEEECCCCCEEHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCCCCCCCCC NSDYVDGDAPPKYDLLTWTTYPERLEANGISWQIYQQGTTGADPLNGNYGTNILQNFANF CCCCCCCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH IDAKPGSSLYRRAQTARTLDDLKADVLANRLPQVSWLLPPAAFSEHPKYTPAYGANYTSQ HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCCCCCCHHHH ILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPATTRAQGLSTVPVDGEIHDVVNPG HHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCHHHCCCEECCCCCCHHHCCCCC RGGSYTADGLPYGLGPRVPMTVVSPWTKGGFVCSQVFDHTSVIRFIAARFGVDEPNITPW CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH RRAVCGDLTSAFDFRTPDATLPPLPDTSNYRSIADQLCSTKPAPSVPAAPSPVDPQEPGV HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC RPARALPYELHVNATVHGGARLRIEFANRGEQGAHFYVYASNRSDGPWRYTVGAHRSLHD CCHHCCCEEEEEEEEEECCEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCC EFDLGATNGAYAFTVYGPNGFVRVFEGLAAAEGHGRHAAAHPEVKAGYDVANGNLYLKLT CCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCEEEEEEE NHGGQSVRLTLADNAYGAPPRQITLRGGDERVEQWALAPSHHWYDVTVSDGATGQFVRRF CCCCCEEEEEEECCCCCCCCCEEEECCCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHH AGHVENGKSSYSDPAAVAPVTG HHHHCCCCCCCCCCCCCCCCCC >Mature Secondary Structure APTTPPAQRRRPRNPPRIQADRFLPPPLPRRVKGENMTSESRRRFLHAVAQSASAAAAL CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE TVFPESIRRALAIPAASRTGTIRDVEHIVVFMQENRSFDHYFGHLRGVRGYNDRFPITLP EECHHHHHHHHCCCCCCCCCCHHHHEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCEECC NGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLDHSWYKTHAAIDGGRYDQWPANKT CCCEEEECCCCCCCCCCCCCEEECCCCCHHHHHHCCCHHHHHHHEEECCCCCCCCCCCCC DMTMGYHVREDIPFHYALADAFTVCDNYFCSLPGPTHPNRSYLMTGTVDPTGKYGGPLLD CEEECEEECCCCCEEHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCCCCCCCCC NSDYVDGDAPPKYDLLTWTTYPERLEANGISWQIYQQGTTGADPLNGNYGTNILQNFANF CCCCCCCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH IDAKPGSSLYRRAQTARTLDDLKADVLANRLPQVSWLLPPAAFSEHPKYTPAYGANYTSQ HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCCCCCCHHHH ILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPATTRAQGLSTVPVDGEIHDVVNPG HHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCHHHCCCEECCCCCCHHHCCCCC RGGSYTADGLPYGLGPRVPMTVVSPWTKGGFVCSQVFDHTSVIRFIAARFGVDEPNITPW CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH RRAVCGDLTSAFDFRTPDATLPPLPDTSNYRSIADQLCSTKPAPSVPAAPSPVDPQEPGV HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC RPARALPYELHVNATVHGGARLRIEFANRGEQGAHFYVYASNRSDGPWRYTVGAHRSLHD CCHHCCCEEEEEEEEEECCEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCC EFDLGATNGAYAFTVYGPNGFVRVFEGLAAAEGHGRHAAAHPEVKAGYDVANGNLYLKLT CCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCEEEEEEE NHGGQSVRLTLADNAYGAPPRQITLRGGDERVEQWALAPSHHWYDVTVSDGATGQFVRRF CCCCCEEEEEEECCCCCCCCCEEEECCCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHH AGHVENGKSSYSDPAAVAPVTG HHHHCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10523590 [H]