| Definition | Burkholderia thailandensis E264 chromosome chromosome I, complete sequence. |
|---|---|
| Accession | NC_007651 |
| Length | 3,809,201 |
Click here to switch to the map view.
The map label for this gene is lgrC [H]
Identifier: 83720112
GI number: 83720112
Start: 2658514
End: 2660745
Strand: Reverse
Name: lgrC [H]
Synonym: BTH_I2360
Alternate gene names: 83720112
Gene position: 2660745-2658514 (Counterclockwise)
Preceding gene: 83720130
Following gene: 83720445
Centisome position: 69.85
GC content: 69.13
Gene sequence:
>2232_bases ATGAAGGCGAAAGTTCAGGACATTCACGCACTCGCGCCGTCGCAGCGCGGGATGCTGCTCGAAAGCGCGCACGGCGCGAT CAAGGGCGGCTTGTTCATCGAGCAGACCGTGCTGTCGGTTCGCGGGCATCTCGATCGCAGCCGTTTCCTCGCGGCCGTCG AGCGCACCGCCGAGACTCACGCGAGCCTGCGCTGTTGTTTCGCATGGGACGCCGAGCAGCCGGTGCAGGTCGTGTTCGAG CGGATTCAGCCCGAAGTGGCATGGGAAGACTGGCGAAACACCGACGACGCGGACAATCCGCAGGCGCTGACGGCTTGGCT GGACGAGGATCGCGCGCGAGGCGTCGCCGTCAACCGCGCGCCGCTGTGGCGCTTCGCCGTGATCCGGCTGCGTGATGACG CATACCGGGTCGTCTGGACTCACCACCATCTCATCACCGACGGCTGGTCGATCGCGGTGGTGCTTTCGGATCTGCTCACC TGCTACGAGGGTGTTCGGCCAGTGCCGCCGGCGCGGCCGCTACGCGATTACGTCAACTGGATCGACGCGCAGGATGCGGC GGCCGCGGCCGCGTTCTGGCGTCAGCGCCTCAGCGCATTGCCCCGCCTGCCCCCGATGGCGCCGCGCGCGTCGGGCGACG GCTTCGCAGAGGTCACGGACATCAACGCGAGCGCGCTCCTCGACGTCGCGCGCGCCCACGGCGTCGCGTTCGCGATTACG GTGTTCGCCGCATGGGCGCTGGTTCTGCGCGCGTTTCGCGCGACCGGTGACGTGGTGTTCGGCGTCACCACGCACGGCCG TCCCGCGACGCTTGCGGGGGTTGAGCGCATCGTCGGGCCGTTCATCTCGACCTTTCCGCTGCATGTGCGGATGACCGATA TGGCAACGGTCGGCGAGTTGCTGGGCCGGATCGAGGCGATGCAGCGCGAGCAGCGCCCGTTCGAATATCTGTCGTCCGCC GAAGTGCATCAGGCCGCCGCCGTTGCCACGCGTCTTTACGATACCTTGGTGGTGGTCGAGCACCTGCCGCAGCAAGACGC GCCCGCCGATGCCGGTTTATCCGTCGCGGCCGAGGCGGGCAGTGGGGCGCGCTCGCATCATCCGTTGCTGTTGCGCGTCT CGCCTGACGGGATCGCGCGCTTGCTGTACAACCGGCGGTTCATCGACGACGCAACGGCGGCCGGGCTGGCGTCGCTGTTG CGCGAGGCGGCGGCGCGACTGGCGGCGGACGATGGTCTGCCGCGCCTCGCAGATCCGGCAATCCCGCTCGCCGTCGCGCA GCCGGCCGCGTCGCGCGCGCCTGAAACCCCGCAAGAGCGGGTGGTCGTCGCGCTCGCGCGCGATCTGTTCGGCGGCCACG CGGGGCTCGACGACAGCTTCTTCGATCTCGGCGGGCATTCGCTGCTCGCCGTCGACTTCCTGCATCGCATCGCGAACGCA ACCGGGGTGCGCTTGCCCTTGACTCAGTTGTTGCACGACGCCCGCCTGGGTGCGATCGCGCAGGCGCTCGCCGAGCGCCT CGCGGGCAACGCCGGCTCCTCCAGCGCACCGCGGATTTTCTTCATCCCCGGCAATCCCGGTTCGCCGTTGTACGCCGCTC GTCTCGCCGCGCATCTGGGCGGCCCGAACGGCTGGCGCTTGCACGGCCTTGAAGCGCCGGGACTCGACGGCGCGCAAGCG CCGTTGCCGAGCGTCGAAGCGCTCGCCGAACACCATCTGGCCAGTCTGTCGGACGCCGACGATCCGGCCGTGCTGGTCGG CCATTCGTTCGGCGGGTGGGTGGCGTTCGAGATGGGGCTGCGCTTGCAGGCAAGTGGCCGGGCGGTGGTGGTGATCCTGC TCGACACGCTCGCCCCACATCGGCTGCCGTCACGCGGGGATGTCGGCGATGCGGGTTTGCGAGCCGAAGTCGCGAGGCTC GCGTCTCGCGCCAACGATGCGGGCTTCGCTATCCATGTCACGCCCGCAGCATTCGCGGTCTATGCCGCCGCGCGGCGCGC GACCTATGCGCCGTCCGCCCGGGCCGAGTTTCCGCTCGCGCTGTTTCGCGCCGCCCGCGCGCACCCCGAGGACGGCTGGG ACAGAAGCGATTCCGCCTATGGCTGGACTGAACTCTGCCGCAAGGTTCATGTGGATTGCGTCGACGGAGATCATCTGTCG ATGATGGCGGAGCCCGCCGTCGAAGTTCTGGCCGAGCGGATCGGGCATTGGCTGCGTCAACTGGGAGGTTGA
Upstream 100 bases:
>100_bases CGCTGCGCCACCGCGGCGACGGGCAGCGCGTGCCGCGCAGTCGGTTGATCGTGCGCTGGCTGCTGAGCCGCGCATCGGAA GTTTGCGGGAGCGCACGGCT
Downstream 100 bases:
>100_bases CGCCCGCGGGCAGTGCGGCCGGGTGCCGCGCATCCGCTTTTCTCAATCGCGGTTTTCCCCTTGTCAAAGGATGAATCATT CATGAATACCGAACGTTCCT
Product: nonribosomal peptide synthetase
Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]
Alternate protein names: ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan/phenylalanine/tyrosine adenylase; Trp/Phe/TyrA; Tryptophan/phenylalanine/tyrosine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing] [H]
Number of amino acids: Translated: 743; Mature: 743
Protein sequence:
>743_residues MKAKVQDIHALAPSQRGMLLESAHGAIKGGLFIEQTVLSVRGHLDRSRFLAAVERTAETHASLRCCFAWDAEQPVQVVFE RIQPEVAWEDWRNTDDADNPQALTAWLDEDRARGVAVNRAPLWRFAVIRLRDDAYRVVWTHHHLITDGWSIAVVLSDLLT CYEGVRPVPPARPLRDYVNWIDAQDAAAAAAFWRQRLSALPRLPPMAPRASGDGFAEVTDINASALLDVARAHGVAFAIT VFAAWALVLRAFRATGDVVFGVTTHGRPATLAGVERIVGPFISTFPLHVRMTDMATVGELLGRIEAMQREQRPFEYLSSA EVHQAAAVATRLYDTLVVVEHLPQQDAPADAGLSVAAEAGSGARSHHPLLLRVSPDGIARLLYNRRFIDDATAAGLASLL REAAARLAADDGLPRLADPAIPLAVAQPAASRAPETPQERVVVALARDLFGGHAGLDDSFFDLGGHSLLAVDFLHRIANA TGVRLPLTQLLHDARLGAIAQALAERLAGNAGSSSAPRIFFIPGNPGSPLYAARLAAHLGGPNGWRLHGLEAPGLDGAQA PLPSVEALAEHHLASLSDADDPAVLVGHSFGGWVAFEMGLRLQASGRAVVVILLDTLAPHRLPSRGDVGDAGLRAEVARL ASRANDAGFAIHVTPAAFAVYAAARRATYAPSARAEFPLALFRAARAHPEDGWDRSDSAYGWTELCRKVHVDCVDGDHLS MMAEPAVEVLAERIGHWLRQLGG
Sequences:
>Translated_743_residues MKAKVQDIHALAPSQRGMLLESAHGAIKGGLFIEQTVLSVRGHLDRSRFLAAVERTAETHASLRCCFAWDAEQPVQVVFE RIQPEVAWEDWRNTDDADNPQALTAWLDEDRARGVAVNRAPLWRFAVIRLRDDAYRVVWTHHHLITDGWSIAVVLSDLLT CYEGVRPVPPARPLRDYVNWIDAQDAAAAAAFWRQRLSALPRLPPMAPRASGDGFAEVTDINASALLDVARAHGVAFAIT VFAAWALVLRAFRATGDVVFGVTTHGRPATLAGVERIVGPFISTFPLHVRMTDMATVGELLGRIEAMQREQRPFEYLSSA EVHQAAAVATRLYDTLVVVEHLPQQDAPADAGLSVAAEAGSGARSHHPLLLRVSPDGIARLLYNRRFIDDATAAGLASLL REAAARLAADDGLPRLADPAIPLAVAQPAASRAPETPQERVVVALARDLFGGHAGLDDSFFDLGGHSLLAVDFLHRIANA TGVRLPLTQLLHDARLGAIAQALAERLAGNAGSSSAPRIFFIPGNPGSPLYAARLAAHLGGPNGWRLHGLEAPGLDGAQA PLPSVEALAEHHLASLSDADDPAVLVGHSFGGWVAFEMGLRLQASGRAVVVILLDTLAPHRLPSRGDVGDAGLRAEVARL ASRANDAGFAIHVTPAAFAVYAAARRATYAPSARAEFPLALFRAARAHPEDGWDRSDSAYGWTELCRKVHVDCVDGDHLS MMAEPAVEVLAERIGHWLRQLGG >Mature_743_residues MKAKVQDIHALAPSQRGMLLESAHGAIKGGLFIEQTVLSVRGHLDRSRFLAAVERTAETHASLRCCFAWDAEQPVQVVFE RIQPEVAWEDWRNTDDADNPQALTAWLDEDRARGVAVNRAPLWRFAVIRLRDDAYRVVWTHHHLITDGWSIAVVLSDLLT CYEGVRPVPPARPLRDYVNWIDAQDAAAAAAFWRQRLSALPRLPPMAPRASGDGFAEVTDINASALLDVARAHGVAFAIT VFAAWALVLRAFRATGDVVFGVTTHGRPATLAGVERIVGPFISTFPLHVRMTDMATVGELLGRIEAMQREQRPFEYLSSA EVHQAAAVATRLYDTLVVVEHLPQQDAPADAGLSVAAEAGSGARSHHPLLLRVSPDGIARLLYNRRFIDDATAAGLASLL REAAARLAADDGLPRLADPAIPLAVAQPAASRAPETPQERVVVALARDLFGGHAGLDDSFFDLGGHSLLAVDFLHRIANA TGVRLPLTQLLHDARLGAIAQALAERLAGNAGSSSAPRIFFIPGNPGSPLYAARLAAHLGGPNGWRLHGLEAPGLDGAQA PLPSVEALAEHHLASLSDADDPAVLVGHSFGGWVAFEMGLRLQASGRAVVVILLDTLAPHRLPSRGDVGDAGLRAEVARL ASRANDAGFAIHVTPAAFAVYAAARRATYAPSARAEFPLALFRAARAHPEDGWDRSDSAYGWTELCRKVHVDCVDGDHLS MMAEPAVEVLAERIGHWLRQLGG
Specific function: Activates the 7th to 12th amino acids (Val, D-Val, Trp, D-Leu, Xaa and D-Leu) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 8th (D-Val), the 10th (D- Leu) an
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 6 acyl carrier domains [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR010071 - InterPro: IPR009081 - InterPro: IPR020845 - InterPro: IPR000873 - InterPro: IPR023213 - InterPro: IPR001242 - InterPro: IPR010060 - InterPro: IPR006163 - InterPro: IPR020806 - InterPro: IPR006162 [H]
Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]
EC number: 2.7.7.- [C]
Molecular weight: Translated: 79787; Mature: 79787
Theoretical pI: Translated: 6.54; Mature: 6.54
Prosite motif: PS50075 ACP_DOMAIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.9 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKAKVQDIHALAPSQRGMLLESAHGAIKGGLFIEQTVLSVRGHLDRSRFLAAVERTAETH CCCCHHHHHHHCCCCCCCCHHHCCCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH ASLRCCFAWDAEQPVQVVFERIQPEVAWEDWRNTDDADNPQALTAWLDEDRARGVAVNRA HCEEEEEECCCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCC PLWRFAVIRLRDDAYRVVWTHHHLITDGWSIAVVLSDLLTCYEGVRPVPPARPLRDYVNW CCEEEEEEEEECCCEEEEEEECEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH IDAQDAAAAAAFWRQRLSALPRLPPMAPRASGDGFAEVTDINASALLDVARAHGVAFAIT HCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHH VFAAWALVLRAFRATGDVVFGVTTHGRPATLAGVERIVGPFISTFPLHVRMTDMATVGEL HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHH LGRIEAMQREQRPFEYLSSAEVHQAAAVATRLYDTLVVVEHLPQQDAPADAGLSVAAEAG HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC SGARSHHPLLLRVSPDGIARLLYNRRFIDDATAAGLASLLREAAARLAADDGLPRLADPA CCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC IPLAVAQPAASRAPETPQERVVVALARDLFGGHAGLDDSFFDLGGHSLLAVDFLHRIANA CCCEECCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHCC TGVRLPLTQLLHDARLGAIAQALAERLAGNAGSSSAPRIFFIPGNPGSPLYAARLAAHLG CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCC GPNGWRLHGLEAPGLDGAQAPLPSVEALAEHHLASLSDADDPAVLVGHSFGGWVAFEMGL CCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECE RLQASGRAVVVILLDTLAPHRLPSRGDVGDAGLRAEVARLASRANDAGFAIHVTPAAFAV EEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHH YAAARRATYAPSARAEFPLALFRAARAHPEDGWDRSDSAYGWTELCRKVHVDCVDGDHLS HHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHH MMAEPAVEVLAERIGHWLRQLGG HHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MKAKVQDIHALAPSQRGMLLESAHGAIKGGLFIEQTVLSVRGHLDRSRFLAAVERTAETH CCCCHHHHHHHCCCCCCCCHHHCCCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH ASLRCCFAWDAEQPVQVVFERIQPEVAWEDWRNTDDADNPQALTAWLDEDRARGVAVNRA HCEEEEEECCCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCC PLWRFAVIRLRDDAYRVVWTHHHLITDGWSIAVVLSDLLTCYEGVRPVPPARPLRDYVNW CCEEEEEEEEECCCEEEEEEECEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH IDAQDAAAAAAFWRQRLSALPRLPPMAPRASGDGFAEVTDINASALLDVARAHGVAFAIT HCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHH VFAAWALVLRAFRATGDVVFGVTTHGRPATLAGVERIVGPFISTFPLHVRMTDMATVGEL HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHH LGRIEAMQREQRPFEYLSSAEVHQAAAVATRLYDTLVVVEHLPQQDAPADAGLSVAAEAG HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC SGARSHHPLLLRVSPDGIARLLYNRRFIDDATAAGLASLLREAAARLAADDGLPRLADPA CCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC IPLAVAQPAASRAPETPQERVVVALARDLFGGHAGLDDSFFDLGGHSLLAVDFLHRIANA CCCEECCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHCC TGVRLPLTQLLHDARLGAIAQALAERLAGNAGSSSAPRIFFIPGNPGSPLYAARLAAHLG CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCC GPNGWRLHGLEAPGLDGAQAPLPSVEALAEHHLASLSDADDPAVLVGHSFGGWVAFEMGL CCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECE RLQASGRAVVVILLDTLAPHRLPSRGDVGDAGLRAEVARLASRANDAGFAIHVTPAAFAV EEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHH YAAARRATYAPSARAEFPLALFRAARAHPEDGWDRSDSAYGWTELCRKVHVDCVDGDHLS HHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHH MMAEPAVEVLAERIGHWLRQLGG HHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: Phosphopantetheine. [C]
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]
Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser
General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA