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The map label for this gene is gidB [H]

Identifier: 83720015

GI number: 83720015

Start: 3779763

End: 3780458

Strand: Reverse

Name: gidB [H]

Synonym: BTH_I3319

Alternate gene names: 83720015

Gene position: 3780458-3779763 (Counterclockwise)

Preceding gene: 83719253

Following gene: 83719053

Centisome position: 99.25

GC content: 62.64

Gene sequence:

>696_bases
ATGACGGCGCAACGTCGTCAAGCCCCGATTGCCAGCCGGGAAACGCTGCAAGCATTGCTATCGGAAGGCGCGGAAGCGCT
GGGCGTCGCGTTGAGCGATGCGCAGCGCGGCGCGTTGCTCGATTATGTCGCGCTGCTCGCCAAGTGGAACGCGGTTTACA
ACCTGACTGCGATCCGTGACCCGAGGCAGATGCTGATTCAACATATCCTCGATTCGCTATCGATCGTCCCGCATCTCGGT
GCGCGGGGGGCGGCGGCGACGGCGCTGGACGTCGGCTCCGGCGGCGGCTTGCCGGGCATCGTGCTCGCGATCGCGCTGCC
CGGCTGGCAGGTCACGCTAAACGACATCGTCCAGAAAAAATCCGCATTCCAGAACCAGGCGAAGGCCGAGTTGAAGCTCG
GCAATCTGTCCGTCGTGACAGGGCGCGTCGAGACGCTGCGTCCTGGTGCCGATGTTCAAGGGAAATTCGACGTGATCGTA
TCGCGGGCGTTCGCGGATCTGGCGGACTTCGTTACACTTGCCCGGCACCTCGTTGCGCCGGGCGGGTCGATTTGGGCGAT
GAAGGGCGTGCGTCCCGATGAAGAAATCGGTCGCTTGCCCGATGGCGCGCGCGTCAAACAGATGATCCGTCTAACGGTCC
CGTCGCTCGATGCGGAACGGCATTTGATCGAAGTCGAGCTCGACGAAGCAATTTAA

Upstream 100 bases:

>100_bases
TGCTAATGGTGCATCTGAAGAAGCGCGGGCTGGGCCGGCGAAAGGGCGCGGACAGTGCGCCGGGCGCCGACGCGGAAACC
AACAGCGCCGCGACGCAGCA

Downstream 100 bases:

>100_bases
TCGGCGCGCCGCAGGGCGCCCGGACCATTTGAAGAGAAGGAATACACCAACGATGGCAAAGATCTTCTGCGTGGCGAACC
AGAAGGGCGGTGTCGGCAAG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTAQRRQAPIASRETLQALLSEGAEALGVALSDAQRGALLDYVALLAKWNAVYNLTAIRDPRQMLIQHILDSLSIVPHLG
ARGAAATALDVGSGGGLPGIVLAIALPGWQVTLNDIVQKKSAFQNQAKAELKLGNLSVVTGRVETLRPGADVQGKFDVIV
SRAFADLADFVTLARHLVAPGGSIWAMKGVRPDEEIGRLPDGARVKQMIRLTVPSLDAERHLIEVELDEAI

Sequences:

>Translated_231_residues
MTAQRRQAPIASRETLQALLSEGAEALGVALSDAQRGALLDYVALLAKWNAVYNLTAIRDPRQMLIQHILDSLSIVPHLG
ARGAAATALDVGSGGGLPGIVLAIALPGWQVTLNDIVQKKSAFQNQAKAELKLGNLSVVTGRVETLRPGADVQGKFDVIV
SRAFADLADFVTLARHLVAPGGSIWAMKGVRPDEEIGRLPDGARVKQMIRLTVPSLDAERHLIEVELDEAI
>Mature_230_residues
TAQRRQAPIASRETLQALLSEGAEALGVALSDAQRGALLDYVALLAKWNAVYNLTAIRDPRQMLIQHILDSLSIVPHLGA
RGAAATALDVGSGGGLPGIVLAIALPGWQVTLNDIVQKKSAFQNQAKAELKLGNLSVVTGRVETLRPGADVQGKFDVIVS
RAFADLADFVTLARHLVAPGGSIWAMKGVRPDEEIGRLPDGARVKQMIRLTVPSLDAERHLIEVELDEAI

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=200, Percent_Identity=43.5, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 24630; Mature: 24499

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQRRQAPIASRETLQALLSEGAEALGVALSDAQRGALLDYVALLAKWNAVYNLTAIRD
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCC
PRQMLIQHILDSLSIVPHLGARGAAATALDVGSGGGLPGIVLAIALPGWQVTLNDIVQKK
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEHEEECCCCEEEHHHHHHHH
SAFQNQAKAELKLGNLSVVTGRVETLRPGADVQGKFDVIVSRAFADLADFVTLARHLVAP
HHHHHHHHHEEEECCEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GGSIWAMKGVRPDEEIGRLPDGARVKQMIRLTVPSLDAERHLIEVELDEAI
CCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
TAQRRQAPIASRETLQALLSEGAEALGVALSDAQRGALLDYVALLAKWNAVYNLTAIRD
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCC
PRQMLIQHILDSLSIVPHLGARGAAATALDVGSGGGLPGIVLAIALPGWQVTLNDIVQKK
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEHEEECCCCEEEHHHHHHHH
SAFQNQAKAELKLGNLSVVTGRVETLRPGADVQGKFDVIVSRAFADLADFVTLARHLVAP
HHHHHHHHHEEEECCEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GGSIWAMKGVRPDEEIGRLPDGARVKQMIRLTVPSLDAERHLIEVELDEAI
CCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA