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The map label for this gene is ligA [H]

Identifier: 83720010

GI number: 83720010

Start: 2290320

End: 2292395

Strand: Direct

Name: ligA [H]

Synonym: BTH_I2022

Alternate gene names: 83720010

Gene position: 2290320-2292395 (Clockwise)

Preceding gene: 83720546

Following gene: 83719851

Centisome position: 60.13

GC content: 68.55

Gene sequence:

>2076_bases
ATGGCCCGATCCCCAGTTGAACCGTCCGCCGGCCAGCCGGCGAAGCGCGCCGCATGGCTGCGCGCCGAGCTCGAGCGGGC
GAACTACGCATATTACGTGCTCGACCAGCCGGACCTGCCCGACGCCGAGTACGACCGCCTCTTCGCCGAACTCCAGCAGA
TCGAGGCCGAGCACCCCGATCTCGTCACGCCCGATTCGCCGACGCAGCGCGTCGGCGGCGAGGTCGCGAGCGGCTTCACG
CCGGTCGTTCACGATACGCCGATGCTGTCGCTCAACAACGGTTTTTCCGACGACGACGTCGTCGCGTTCGACAAGCGCGT
CGCCGACGGCCTCGACAAGCCGACCGACCTCGCCGGCACCGTGACCGAGCCTGTCGAATACGCGTGCGAGCTGAAGTTCG
ACGGCCTGGCGATCTCGCTGCGCTACGAGAACGGCCGCTTCGTGCAGGCATCGACGCGCGGCGACGGCACGACGGGCGAA
GACGTCACCGAGAACATCCGCACGGTCCGCTCGATTCCGCTGACGCTCAAGGGCAAGCGCGTGCCGCGCATGCTCGACGT
GCGCGGCGAAGTGCTCATGTTCAGGCGCGATTTCGCGCGCTTGAACGAGCGTCAGCGCGCGGCGGGCCAGCGCGAATTCG
CCAATCCGCGCAACGCGGCGGCGGGTAGCCTGCGGCAGCTCGATTCGAAGATCACCGCGTCGCGGCCGCTTTCGTTTTTC
GCCTACGGGATCGGCGTGCTCGACGGCGTCGAGATGCCCGACACGCACAGCAGCCTGCTCGACTGGTACGAGACGCTCGG
GCTGCCGGTGAACCGCGAGCGCGCGGTCGTGCGCGGCGCGGCGGGCCTGCTCGAGTTCTTCCATTCGGTGGGCGCGCGGC
GCGAGTCGCTGCCGTACGACATCGACGGCGTCGTCTACAAGGTGAATCGCCGCGACGAGCAGGATCGGCTCGGCTTCGTG
TCGCGCGCGCCGCGCTTCGCGCTCGCGCACAAGTTCCCCGCGCAGGAGGCGCTGACGAAGCTCGTCGCGATCGACGTGCA
GGTCGGCCGCACGGGCGCGATCACGCCGGTCGCGCGTCTCGAGCCCGTGTTCGTCGGCGGCGCGACCGTCACGAACGCGA
CGCTGCACAACGAGGACGAGGTGCGCCGCAAGGACATCCGGATCGGCGACACGGTCATCGTGCGCCGCGCGGGCGACGTG
ATTCCCGAAGTGGTGTCGGCCGTGTTCGACCGGCGTCCCGCCGACGCGCGCGAGTTCGTGATGCCGACCGAGTGCCCGGA
ATGCGGTTCGCGGGTCGAGCGATTGCCCGACGAGGCGATCGCGCGCTGCACGGGCGGGCTCTTCTGCCCCGCGCAGCGCA
AGCAGGCGCTCTGGCACTTCGCGCAGCGCCGCGCGCTCGACATCGACGGGCTCGGCGAGAAGATCATCGATCAGCTCGTC
GAGCAGAACCTCGTGCGCACGCCGGCCGATCTGTTCAATCTCGGCTTCTCGACGCTCGTCGAGCTCGATCGTTTTGCGGA
GAAGTCCGCGCAGAACCTGATCGACTCGCTCGAAAAGGCGAAGCACACGACGCTCGCGCGCTTCATTTACGCGCTCGGCA
TCCGGCACGTCGGCGAATCGACGGCGAAGGACCTCGCGAAGCACTTCGGCTCGCTCGATCCGATCATGGACGCGTCGATC
GACGAATTGCTCGAAGTCAATGACGTCGGGCCGATCGTCGCCGAATCGATCCATCAGTTCTTCGCCGAGGCGCACAATCG
CACGGTGATCGAGCAACTGCGCGCGAAGGGCAAAGTCACGTGGCCCGAAGGGCCGCCCGCGCCGCGCGCGCCGCAGGGCG
TGCTCGCGGGCAAGACCGTCGTGCTGACGGGCACGCTGCCGACGCTCACGCGGGAAGCCGCGAAGGAGATGCTCGAGGCG
GCGGGCGCGAAGGTCGCGGGCTCGGTGTCGAAGAAGACCGATTACGTCGTCGCGGGCGCCGACGCGGGCAGCAAGCTCGC
GAAAGCCGAGGAGCTCGGCATTCCGGTGCTGGACGAAGCAGGCATGCACAAACTTCTGGAGGGCCATGCGCGATGA

Upstream 100 bases:

>100_bases
CCGCGATTTCCGCGATCGACCCGCCAATCAGCCGATGCCGAAAAAGGCTCGAACTGAGATAATCGTCGCCTGATGATCCT
CACGAGAAAGTGCCGCCAGC

Downstream 100 bases:

>100_bases
TTCGCGAGATTCTGAAGATGGGCGATCCGCGTCTGCTGGAAGTCGCCCGGCCGGTGGAGTCGTTCAATACGCCGGAGTTG
CACGCGCTCGTCGCGGACAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MARSPVEPSAGQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFAELQQIEAEHPDLVTPDSPTQRVGGEVASGFT
PVVHDTPMLSLNNGFSDDDVVAFDKRVADGLDKPTDLAGTVTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGE
DVTENIRTVRSIPLTLKGKRVPRMLDVRGEVLMFRRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFF
AYGIGVLDGVEMPDTHSSLLDWYETLGLPVNRERAVVRGAAGLLEFFHSVGARRESLPYDIDGVVYKVNRRDEQDRLGFV
SRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVSAVFDRRPADAREFVMPTECPECGSRVERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
EQNLVRTPADLFNLGFSTLVELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDASI
DELLEVNDVGPIVAESIHQFFAEAHNRTVIEQLRAKGKVTWPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEA
AGAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGMHKLLEGHAR

Sequences:

>Translated_691_residues
MARSPVEPSAGQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFAELQQIEAEHPDLVTPDSPTQRVGGEVASGFT
PVVHDTPMLSLNNGFSDDDVVAFDKRVADGLDKPTDLAGTVTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGE
DVTENIRTVRSIPLTLKGKRVPRMLDVRGEVLMFRRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFF
AYGIGVLDGVEMPDTHSSLLDWYETLGLPVNRERAVVRGAAGLLEFFHSVGARRESLPYDIDGVVYKVNRRDEQDRLGFV
SRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVSAVFDRRPADAREFVMPTECPECGSRVERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
EQNLVRTPADLFNLGFSTLVELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDASI
DELLEVNDVGPIVAESIHQFFAEAHNRTVIEQLRAKGKVTWPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEA
AGAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGMHKLLEGHAR
>Mature_690_residues
ARSPVEPSAGQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFAELQQIEAEHPDLVTPDSPTQRVGGEVASGFTP
VVHDTPMLSLNNGFSDDDVVAFDKRVADGLDKPTDLAGTVTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGED
VTENIRTVRSIPLTLKGKRVPRMLDVRGEVLMFRRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFFA
YGIGVLDGVEMPDTHSSLLDWYETLGLPVNRERAVVRGAAGLLEFFHSVGARRESLPYDIDGVVYKVNRRDEQDRLGFVS
RAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVI
PEVVSAVFDRRPADAREFVMPTECPECGSRVERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVE
QNLVRTPADLFNLGFSTLVELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDASID
ELLEVNDVGPIVAESIHQFFAEAHNRTVIEQLRAKGKVTWPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEAA
GAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGMHKLLEGHAR

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=42.8571428571429, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=57.2496263079223, Blast_Score=756, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=524, Percent_Identity=23.0916030534351, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324791, Length=90, Percent_Identity=42.2222222222222, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75657; Mature: 75525

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSPVEPSAGQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFAELQQIEAEHPD
CCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
LVTPDSPTQRVGGEVASGFTPVVHDTPMLSLNNGFSDDDVVAFDKRVADGLDKPTDLAGT
CCCCCCCHHHHCCHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCC
VTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGEDVTENIRTVRSIPLTLKGKR
HHHHHHHHHEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCC
VPRMLDVRGEVLMFRRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFF
CCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
AYGIGVLDGVEMPDTHSSLLDWYETLGLPVNRERAVVRGAAGLLEFFHSVGARRESLPYD
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
IDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARL
CCCEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVSAVFDRRPADAREFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPECGSRVERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EQNLVRTPADLFNLGFSTLVELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGES
HHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLDPIMDASIDELLEVNDVGPIVAESIHQFFAEAHNRTVIEQLRAKGKVT
HHHHHHHHCCCCCCHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCEE
WPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEAAGAKVAGSVSKKTDYVVAGA
CCCCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
DAGSKLAKAEELGIPVLDEAGMHKLLEGHAR
CCCHHHHHHHHCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
ARSPVEPSAGQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFAELQQIEAEHPD
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
LVTPDSPTQRVGGEVASGFTPVVHDTPMLSLNNGFSDDDVVAFDKRVADGLDKPTDLAGT
CCCCCCCHHHHCCHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCC
VTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGEDVTENIRTVRSIPLTLKGKR
HHHHHHHHHEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCC
VPRMLDVRGEVLMFRRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFF
CCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
AYGIGVLDGVEMPDTHSSLLDWYETLGLPVNRERAVVRGAAGLLEFFHSVGARRESLPYD
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
IDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARL
CCCEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVSAVFDRRPADAREFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPECGSRVERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EQNLVRTPADLFNLGFSTLVELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGES
HHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLDPIMDASIDELLEVNDVGPIVAESIHQFFAEAHNRTVIEQLRAKGKVT
HHHHHHHHCCCCCCHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCEE
WPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEAAGAKVAGSVSKKTDYVVAGA
CCCCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
DAGSKLAKAEELGIPVLDEAGMHKLLEGHAR
CCCHHHHHHHHCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA